DOI: 10.1083/jcb.20.1.165. THE PROBLEM OF DEMINERALISATION IN THIN SECTIONS OF FULLY CALCIFIED BONE

THE PROBLEM OF DEMINERALISATION IN THIN SECTIONS OF FULLY CALCIFIED BONE

10.1083/jcb.20.1.165

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

Thin sections of embryonic avian bone decalcify during preparation for electron microscopy, creating a false impression of mineral distribution. The results of the experiments reported herein show that viscous embedding materials do not penetrate compact formed bone, and so, in thin sections, the calcium apatite crystals may be leeched out by water, both in the collecting trough and in aqueous solutions of stains used to enhance tissue electron opacity. To prevent decalcification, a simple technique is described in which the aqueous fluids that come in contact with thin sections are saturated with respect to calcium and phosphate ions, thereby preventing solution of the bone mineral. The theoretical basis of this technique is briefly discussed.

Bibliography

Boothroyd, B. (1964). THE PROBLEM OF DEMINERALISATION IN THIN SECTIONS OF FULLY CALCIFIED BONE. The Journal of Cell Biology, 20(1), 165â173.

Authors 1

B. Boothroyd (first)

References 0 Referenced 109

None

Dates

Type	When
Created	21 years, 3 months ago (May 14, 2004, 1:40 p.m.)
Deposited	2 years, 1 month ago (July 22, 2023, 11:09 p.m.)
Indexed	2 months, 2 weeks ago (June 18, 2025, 4:27 a.m.)
Issued	61 years, 8 months ago (Jan. 1, 1964)
Published	61 years, 8 months ago (Jan. 1, 1964)
Published Online	61 years, 8 months ago (Jan. 1, 1964)
Published Print	61 years, 8 months ago (Jan. 1, 1964)

Funders 0

None

BibTeX

@article{Boothroyd_1964, title={THE PROBLEM OF DEMINERALISATION IN THIN SECTIONS OF FULLY CALCIFIED BONE}, volume={20}, ISSN={0021-9525}, url={http://dx.doi.org/10.1083/jcb.20.1.165}, DOI={10.1083/jcb.20.1.165}, number={1}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Boothroyd, B.}, year={1964}, month=jan, pages={165–173} }

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