DOI: 10.1083/jcb.153.7.1465. Nup2p Dynamically Associates with the Distal Regions of the Yeast Nuclear Pore Complex

Nup2p Dynamically Associates with the Distal Regions of the Yeast Nuclear Pore Complex

10.1083/jcb.153.7.1465

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

Nucleocytoplasmic transport is mediated by the interplay between soluble transport factors and nucleoporins resident within the nuclear pore complex (NPC). Understanding this process demands knowledge of components of both the soluble and stationary phases and the interface between them. Here, we provide evidence that Nup2p, previously considered to be a typical yeast nucleoporin that binds import- and export-bound karyopherins, dynamically associates with the NPC in a Ran-facilitated manner. When bound to the NPC, Nup2p associates with regions corresponding to the nuclear basket and cytoplasmic fibrils. On the nucleoplasmic face, where the Ran–GTP levels are predicted to be high, Nup2p binds to Nup60p. Deletion of NUP60 renders Nup2p nucleoplasmic and compromises Nup2p-mediated recycling of Kap60p/Srp1p. Depletion of Ran–GTP by metabolic poisoning, disruption of the Ran cycle, or in vitro by cell lysis, results in a shift of Nup2p from the nucleoplasm to the cytoplasmic face of the NPC. This mobility of Nup2p was also detected using heterokaryons where, unlike nucleoporins, Nup2p was observed to move from one nucleus to the other. Together, our data support a model in which Nup2p movement facilitates the transition between the import and export phases of nucleocytoplasmic transport.

Bibliography

Dilworth, D. J., Suprapto, A., Padovan, J. C., Chait, B. T., Wozniak, R. W., Rout, M. P., & Aitchison, J. D. (2001). Nup2p Dynamically Associates with the Distal Regions of the Yeast Nuclear Pore Complex. The Journal of Cell Biology, 153(7), 1465â1478.

Authors 7

David J. Dilworth (first)
Adisetyantari Suprapto (additional)
Julio C. Padovan (additional)
Brian T. Chait (additional)
Richard W. Wozniak (additional)
Michael P. Rout (additional)
John D. Aitchison (additional)

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 12:46 p.m.)
Deposited	2 years, 1 month ago (July 28, 2023, 11 p.m.)
Indexed	3 weeks, 1 day ago (Aug. 7, 2025, 4:50 p.m.)
Issued	24 years, 2 months ago (June 25, 2001)
Published	24 years, 2 months ago (June 25, 2001)
Published Online	24 years, 2 months ago (June 25, 2001)
Published Print	24 years, 2 months ago (June 25, 2001)

Funders 0

None

BibTeX

@article{Dilworth_2001, title={Nup2p Dynamically Associates with the Distal Regions of the Yeast Nuclear Pore Complex}, volume={153}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.153.7.1465}, DOI={10.1083/jcb.153.7.1465}, number={7}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Dilworth, David J. and Suprapto, Adisetyantari and Padovan, Julio C. and Chait, Brian T. and Wozniak, Richard W. and Rout, Michael P. and Aitchison, John D.}, year={2001}, month=jun, pages={1465–1478} }

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