DOI: 10.1083/jcb.153.7.1453. Muscle Activity and Muscle Agrin Regulate the Organization of Cytoskeletal Proteins and Attached Acetylcholine Receptor (Achr) Aggregates in Skeletal Muscle Fibers

Muscle Activity and Muscle Agrin Regulate the Organization of Cytoskeletal Proteins and Attached Acetylcholine Receptor (Achr) Aggregates in Skeletal Muscle Fibers

10.1083/jcb.153.7.1453

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

In innervated skeletal muscle fibers, dystrophin and β-dystroglycan form rib-like structures (costameres) that appear as predominantly transverse stripes over Z and M lines. Here, we show that the orientation of these stripes becomes longitudinal in denervated muscles and transverse again in denervated electrically stimulated muscles. Skeletal muscle fibers express nonneural (muscle) agrin whose function is not well understood. In this work, a single application of ≥10 nM purified recombinant muscle agrin into denervated muscles preserved the transverse orientation of costameric proteins that is typical for innervated muscles, as did a single application of ≥1 μM neural agrin. At lower concentration, neural agrin induced acetylcholine receptor aggregates, which colocalized with longitudinally oriented β-dystroglycan, dystrophin, utrophin, syntrophin, rapsyn, and β2-laminin in denervated unstimulated fibers and with the same but transversely oriented proteins in innervated or denervated stimulated fibers. The results indicate that costameres are plastic structures whose organization depends on electrical muscle activity and/or muscle agrin.

Bibliography

Bezakova, G., & LÃ¸mo, T. (2001). Muscle Activity and Muscle Agrin Regulate the Organization of Cytoskeletal Proteins and Attached Acetylcholine Receptor (Achr) Aggregates in Skeletal Muscle Fibers. The Journal of Cell Biology, 153(7), 1453â1464.

Authors 2

Gabriela Bezakova (first)
Terje Lømo (additional)

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 12:46 p.m.)
Deposited	2 years, 1 month ago (July 28, 2023, 10:56 p.m.)
Indexed	1 month, 3 weeks ago (July 7, 2025, 2:27 a.m.)
Issued	24 years, 2 months ago (June 25, 2001)
Published	24 years, 2 months ago (June 25, 2001)
Published Online	24 years, 2 months ago (June 25, 2001)
Published Print	24 years, 2 months ago (June 25, 2001)

Funders 0

None

BibTeX

@article{Bezakova_2001, title={Muscle Activity and Muscle Agrin Regulate the Organization of Cytoskeletal Proteins and Attached Acetylcholine Receptor (Achr) Aggregates in Skeletal Muscle Fibers}, volume={153}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.153.7.1453}, DOI={10.1083/jcb.153.7.1453}, number={7}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Bezakova, Gabriela and Lømo, Terje}, year={2001}, month=jun, pages={1453–1464} }

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