DOI: 10.1083/jcb.153.7.1441. Effects of Purified Recombinant Neural and Muscle Agrin on Skeletal Muscle Fibers in Vivo

Effects of Purified Recombinant Neural and Muscle Agrin on Skeletal Muscle Fibers in Vivo

10.1083/jcb.153.7.1441

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

Aggregation of acetylcholine receptors (AChRs) in muscle fibers by nerve-derived agrin plays a key role in the formation of neuromuscular junctions. So far, the effects of agrin on muscle fibers have been studied in culture systems, transgenic animals, and in animals injected with agrin–cDNA constructs. We have applied purified recombinant chick neural and muscle agrin to rat soleus muscle in vivo and obtained the following results. Both neural and muscle agrin bind uniformly to the surface of innervated and denervated muscle fibers along their entire length. Neural agrin causes a dose-dependent appearance of AChR aggregates, which persist ≥7 wk after a single application. Muscle agrin does not cluster AChRs and at 10 times the concentration of neural agrin does not reduce binding or AChR-aggregating activity of neural agrin. Electrical muscle activity affects the stability of agrin binding and the number, size, and spatial distribution of the neural agrin–induced AChR aggregates. Injected agrin is recovered from the muscles together with laminin and both proteins coimmunoprecipitate, indicating that agrin binds to laminin in vivo. Thus, the present approach provides a novel, simple, and efficient method for studying the effects of agrin on muscle under controlled conditions in vivo.

Bibliography

Bezakova, G., Helm, J. P., Francolini, M., & LÃ¸mo, T. (2001). Effects of Purified Recombinant Neural and Muscle Agrin on Skeletal Muscle Fibers in Vivo. The Journal of Cell Biology, 153(7), 1441â1452.

Authors 4

Gabriela Bezakova (first)
Johannes P. Helm (additional)
Maura Francolini (additional)
Terje Lømo (additional)

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 12:46 p.m.)
Deposited	2 years, 1 month ago (July 28, 2023, 10:59 p.m.)
Indexed	5 days, 21 hours ago (Aug. 31, 2025, 6:02 a.m.)
Issued	24 years, 2 months ago (June 25, 2001)
Published	24 years, 2 months ago (June 25, 2001)
Published Online	24 years, 2 months ago (June 25, 2001)
Published Print	24 years, 2 months ago (June 25, 2001)

Funders 0

None

BibTeX

@article{Bezakova_2001, title={Effects of Purified Recombinant Neural and Muscle Agrin on Skeletal Muscle Fibers in Vivo}, volume={153}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.153.7.1441}, DOI={10.1083/jcb.153.7.1441}, number={7}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Bezakova, Gabriela and Helm, Johannes P. and Francolini, Maura and Lømo, Terje}, year={2001}, month=jun, pages={1441–1452} }

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