DOI: 10.1083/jcb.153.7.1341. Kinetics of Core Histones in Living Human Cells

Kinetics of Core Histones in Living Human Cells

10.1083/jcb.153.7.1341

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

Histones H2A and H2B form part of the same nucleosomal structure as H3 and H4. Stable HeLa cell lines expressing histones H2B, H3, and H4 tagged with green fluorescent protein (GFP) were established; the tagged molecules were assembled into nucleosomes. Although H2B-GFP was distributed like DNA, H3-GFP and H4-GFP were concentrated in euchromatin during interphase and in R-bands in mitotic chromosomes. These differences probably result from an unregulated production of tagged histones and differences in exchange. In both single cells and heterokaryons, photobleaching revealed that H2B-GFP exchanged more rapidly than H3-GFP and H4-GFP. About 3% of H2B exchanged within minutes, whereas ∼40% did so slowly (t1/2 ∼ 130 min). The rapidly exchanging fraction disappeared in 5,6-dichloro-1-β-d-ribofuranosylbenzimidazole and so may represent H2B in transcriptionally active chromatin. The slowly exchanging fraction was probably associated with chromatin domains surrounding active units. H3-GFP and H4-GFP were assembled into chromatin when DNA was replicated, and then &gt;80% remained bound permanently. These results reveal that the inner core of the nucleosome is very stable, whereas H2B on the surface of active nucleosomes exchanges continually.

Bibliography

Kimura, H., & Cook, P. R. (2001). Kinetics of Core Histones in Living Human Cells. The Journal of Cell Biology, 153(7), 1341â1354.

Authors 2

Hiroshi Kimura (first)
Peter R. Cook (additional)

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 12:46 p.m.)
Deposited	2 years ago (July 28, 2023, 10:56 p.m.)
Indexed	12 minutes ago (Aug. 27, 2025, 7:16 a.m.)
Issued	24 years, 2 months ago (June 18, 2001)
Published	24 years, 2 months ago (June 18, 2001)
Published Online	24 years, 2 months ago (June 18, 2001)
Published Print	24 years, 2 months ago (June 25, 2001)

Funders 0

None

BibTeX

@article{Kimura_2001, title={Kinetics of Core Histones in Living Human Cells}, volume={153}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.153.7.1341}, DOI={10.1083/jcb.153.7.1341}, number={7}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Kimura, Hiroshi and Cook, Peter R.}, year={2001}, month=jun, pages={1341–1354} }

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