DOI: 10.1083/jcb.153.6.1227. Him-10 Is Required for Kinetochore Structure and Function on <i>Caenorhabditis elegans</i> Holocentric Chromosomes

Him-10 Is Required for Kinetochore Structure and Function on Caenorhabditis elegans Holocentric Chromosomes

10.1083/jcb.153.6.1227

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

Macromolecular structures called kinetochores attach and move chromosomes within the spindle during chromosome segregation. Using electron microscopy, we identified a structure on the holocentric mitotic and meiotic chromosomes of Caenorhabditis elegans that resembles the mammalian kinetochore. This structure faces the poles on mitotic chromosomes but encircles meiotic chromosomes. Worm kinetochores require the evolutionarily conserved HIM-10 protein for their structure and function. HIM-10 localizes to the kinetochores and mediates attachment of chromosomes to the spindle. Depletion of HIM-10 disrupts kinetochore structure, causes a failure of bipolar spindle attachment, and results in chromosome nondisjunction. HIM-10 is related to the Nuf2 kinetochore proteins conserved from yeast to humans. Thus, the extended kinetochores characteristic of C. elegans holocentric chromosomes provide a guide to the structure, molecular architecture, and function of conventional kinetochores.

Bibliography

Howe, M., McDonald, K. L., Albertson, D. G., & Meyer, B. J. (2001). Him-10 Is Required for Kinetochore Structure and Function on Caenorhabditis elegans Holocentric Chromosomes. The Journal of Cell Biology, 153(6), 1227â1238.

Authors 4

Mary Howe (first)
Kent L. McDonald (additional)
Donna G. Albertson (additional)
Barbara J. Meyer (additional)

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 12:46 p.m.)
Deposited	2 years, 1 month ago (July 22, 2023, 10:23 a.m.)
Indexed	4 weeks, 2 days ago (Aug. 6, 2025, 9:05 a.m.)
Issued	24 years, 2 months ago (June 11, 2001)
Published	24 years, 2 months ago (June 11, 2001)
Published Online	24 years, 2 months ago (June 11, 2001)
Published Print	24 years, 2 months ago (June 11, 2001)

Funders 0

None

BibTeX

@article{Howe_2001, title={Him-10 Is Required for Kinetochore Structure and Function on Caenorhabditis elegans Holocentric Chromosomes}, volume={153}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.153.6.1227}, DOI={10.1083/jcb.153.6.1227}, number={6}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Howe, Mary and McDonald, Kent L. and Albertson, Donna G. and Meyer, Barbara J.}, year={2001}, month=jun, pages={1227–1238} }

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