DOI: 10.1083/jcb.153.3.621. Mistargeting of B-Type Lamins at the End of Mitosis

Mistargeting of B-Type Lamins at the End of Mitosis

10.1083/jcb.153.3.621

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

We previously showed that targeting of protein phosphatase 1 (PP1) to the nuclear envelope (NE) by the A-kinase anchoring protein, AKAP149, correlates with nuclear assembly of B-type lamins in vitro. We demonstrate here that failure of AKAP149-mediated assembly of B-type lamins into the nuclear lamina at the end of mitosis is followed by apoptosis, and induces expression of the gene encoding A-type lamins in cells that normally do not express lamins A/C. In HeLa cells, inhibition of PP1 association with the NE mediated by a peptide containing the PP1-binding domain of AKAP149 results in failure of B-type lamins to assemble, and in their rapid caspase-dependent proteolysis. However, assembly of lamins A/C is not affected. Nonetheless, apoptosis follows within hours of nuclear reformation after mitosis. In lymphoid KE37 cells, which do not express lamins A/C, inhibition of B-type lamin assembly triggers rapid synthesis and nuclear assembly of both lamins A and C before apoptosis takes place. The results indicate that nuclear assembly of B-type lamins is essential for cell survival. They also suggest that mistargeting of B-type lamins at the end of mitosis elicits a tentative rescue process to assemble a nuclear lamina in lymphoid cells that normally do not express lamins A/C.

Bibliography

Steen, R. L., & Collas, P. (2001). Mistargeting of B-Type Lamins at the End of Mitosis. The Journal of Cell Biology, 153(3), 621â626.

Authors 2

Rikke L. Steen (first)
Philippe Collas (additional)

References 26 Referenced 80

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10.1016/S0962-8924(99)01708-0 / Trends Cell Biol. / The nuclear envelope, muscular dystrophy and gene expression by Wilson (2000)

Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 12:46 p.m.)
Deposited	2 years ago (July 28, 2023, 10:56 p.m.)
Indexed	1 month, 3 weeks ago (July 2, 2025, 2:31 p.m.)
Issued	24 years, 3 months ago (April 30, 2001)
Published	24 years, 3 months ago (April 30, 2001)
Published Online	24 years, 3 months ago (April 30, 2001)
Published Print	24 years, 3 months ago (April 30, 2001)

Funders 0

None

BibTeX

@article{Steen_2001, title={Mistargeting of B-Type Lamins at the End of Mitosis}, volume={153}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.153.3.621}, DOI={10.1083/jcb.153.3.621}, number={3}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Steen, Rikke L. and Collas, Philippe}, year={2001}, month=apr, pages={621–626} }

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