DOI: 10.1083/jcb.153.2.397. A Mechanism for Nuclear Positioning in Fission Yeast Based on Microtubule Pushing

A Mechanism for Nuclear Positioning in Fission Yeast Based on Microtubule Pushing

10.1083/jcb.153.2.397

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

The correct positioning of the nucleus is often important in defining the spatial organization of the cell, for example, in determining the cell division plane. In interphase Schizosaccharomyces pombe cells, the nucleus is positioned in the middle of the cylindrical cell in an active microtubule (MT)-dependent process. Here, we used green fluorescent protein markers to examine the dynamics of MTs, spindle pole body, and the nuclear envelope in living cells. We find that interphase MTs are organized in three to four antiparallel MT bundles arranged along the long axis of the cell, with MT plus ends facing both the cell tips and minus ends near the middle of the cell. The MT bundles are organized from medial MT-organizing centers that may function as nuclear attachment sites. When MTs grow to the cell tips, they exert transient forces produced by plus end MT polymerization that push the nucleus. After an average of 1.5 min of growth at the cell tip, MT plus ends exhibit catastrophe and shrink back to the nuclear region before growing back to the cell tip. Computer modeling suggests that a balance of these pushing MT forces can provide a mechanism to position the nucleus at the middle of the cell.

Bibliography

Tran, P. T., Marsh, L., Doye, V., InouÃ©, S., & Chang, F. (2001). A Mechanism for Nuclear Positioning in Fission Yeast Based on Microtubule Pushing. The Journal of Cell Biology, 153(2), 397â412.

Authors 5

P.T. Tran (first)
L. Marsh (additional)
V. Doye (additional)
S. Inoué (additional)
F. Chang (additional)

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Dates

Type	When
Created	23 years ago (July 26, 2002, 12:46 p.m.)
Deposited	2 years ago (July 28, 2023, 11 p.m.)
Indexed	2 weeks, 4 days ago (Aug. 6, 2025, 9:26 a.m.)
Issued	24 years, 4 months ago (April 16, 2001)
Published	24 years, 4 months ago (April 16, 2001)
Published Online	24 years, 4 months ago (April 16, 2001)
Published Print	24 years, 4 months ago (April 16, 2001)

Funders 0

None

BibTeX

@article{Tran_2001, title={A Mechanism for Nuclear Positioning in Fission Yeast Based on Microtubule Pushing}, volume={153}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.153.2.397}, DOI={10.1083/jcb.153.2.397}, number={2}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Tran, P.T. and Marsh, L. and Doye, V. and Inoué, S. and Chang, F.}, year={2001}, month=apr, pages={397–412} }

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