DOI: 10.1083/jcb.153.2.351. Mammalian Abp1, a Signal-Responsive F-Actin–Binding Protein, Links the Actin Cytoskeleton to Endocytosis via the Gtpase Dynamin

Mammalian Abp1, a Signal-Responsive F-Actin–Binding Protein, Links the Actin Cytoskeleton to Endocytosis via the Gtpase Dynamin

10.1083/jcb.153.2.351

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

The actin cytoskeleton has been implicated in endocytosis, yet few molecular links to the endocytic machinery have been established. Here we show that the mammalian F-actin–binding protein Abp1 (SH3P7/HIP-55) can functionally link the actin cytoskeleton to dynamin, a GTPase that functions in endocytosis. Abp1 binds directly to dynamin in vitro through its SH3 domain. Coimmunoprecipitation and colocalization studies demonstrated the in vivo relevance of this interaction. In neurons, mammalian Abp1 and dynamin colocalized at actin-rich sites proximal to the cell body during synaptogenesis. In fibroblasts, mAbp1 appeared at dynamin-rich sites of endocytosis upon growth factor stimulation. To test whether Abp1 functions in endocytosis, we overexpressed several Abp1 constructs in Cos-7 cells and assayed receptor-mediated endocytosis. While overexpression of Abp1's actin-binding modules did not interfere with endocytosis, overexpression of the SH3 domain led to a potent block of transferrin uptake. This implicates the Abp1/dynamin interaction in endocytic function. The endocytosis block was rescued by cooverexpression of dynamin. Since the addition of the actin-binding modules of Abp1 to the SH3 domain construct also fully restored endocytosis, Abp1 may support endocytosis by combining its SH3 domain interactions with cytoskeletal functions in response to signaling cascades converging on this linker protein.

Bibliography

Kessels, M. M., Engqvist-Goldstein, Ã. E. Y., Drubin, D. G., & Qualmann, B. (2001). Mammalian Abp1, a Signal-Responsive F-ActinâBinding Protein, Links the Actin Cytoskeleton to Endocytosis via the Gtpase Dynamin. The Journal of Cell Biology, 153(2), 351â366.

Authors 4

Michael M. Kessels (first)
Åsa E.Y. Engqvist-Goldstein (additional)
David G. Drubin (additional)
Britta Qualmann (additional)

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 12:46 p.m.)
Deposited	2 years ago (July 28, 2023, 10:59 p.m.)
Indexed	1 day, 10 hours ago (Aug. 27, 2025, 12:09 p.m.)
Issued	24 years, 4 months ago (April 16, 2001)
Published	24 years, 4 months ago (April 16, 2001)
Published Online	24 years, 4 months ago (April 16, 2001)
Published Print	24 years, 4 months ago (April 16, 2001)

Funders 0

None

BibTeX

@article{Kessels_2001, title={Mammalian Abp1, a Signal-Responsive F-Actin–Binding Protein, Links the Actin Cytoskeleton to Endocytosis via the Gtpase Dynamin}, volume={153}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.153.2.351}, DOI={10.1083/jcb.153.2.351}, number={2}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Kessels, Michael M. and Engqvist-Goldstein, Åsa E.Y. and Drubin, David G. and Qualmann, Britta}, year={2001}, month=apr, pages={351–366} }

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