DOI: 10.1083/jcb.153.2.307. An Exclusively Nuclear RNA-Binding Protein Affects Asymmetric Localization of <i>ASH1</i> mRNA and Ash1p in Yeast

An Exclusively Nuclear RNA-Binding Protein Affects Asymmetric Localization of ASH1 mRNA and Ash1p in Yeast

10.1083/jcb.153.2.307

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

The localization of ASH1 mRNA to the distal tip of budding yeast cells is essential for the proper regulation of mating type switching in Saccharomyces cerevisiae. A localization element that is predominantly in the 3′-untranslated region (UTR) can direct this mRNA to the bud. Using this element in the three-hybrid in vivo RNA-binding assay, we identified a protein, Loc1p, that binds in vitro directly to the wild-type ASH1 3′-UTR RNA, but not to a mutant RNA incapable of localizing to the bud nor to several other mRNAs. LOC1 codes for a novel protein that recognizes double-stranded RNA structures and is required for efficient localization of ASH1 mRNA. Accordingly, Ash1p gets symmetrically distributed between daughter and mother cells in a loc1 strain. Surprisingly, Loc1p was found to be strictly nuclear, unlike other known RNA-binding proteins involved in mRNA localization which shuttle between the nucleus and the cytoplasm. We propose that efficient cytoplasmic ASH1 mRNA localization requires a previous interaction with specific nuclear factors.

Bibliography

Long, R. M., Gu, W., Meng, X., Gonsalvez, G., Singer, R. H., & Chartrand, P. (2001). An Exclusively Nuclear RNA-Binding Protein Affects Asymmetric Localization of ASH1 mRNA and Ash1p in Yeast. The Journal of Cell Biology, 153(2), 307â318.

Authors 6

Roy M. Long (first)
Wei Gu (additional)
Xiuhua Meng (additional)
Graydon Gonsalvez (additional)
Robert H. Singer (additional)
Pascal Chartrand (additional)

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Dates

Type	When
Created	23 years ago (July 26, 2002, 12:46 p.m.)
Deposited	2 years ago (July 28, 2023, 10:55 p.m.)
Indexed	9 months ago (Nov. 19, 2024, 10:42 a.m.)
Issued	24 years, 4 months ago (April 16, 2001)
Published	24 years, 4 months ago (April 16, 2001)
Published Online	24 years, 4 months ago (April 16, 2001)
Published Print	24 years, 4 months ago (April 16, 2001)

Funders 0

None

BibTeX

@article{Long_2001, title={An Exclusively Nuclear RNA-Binding Protein Affects Asymmetric Localization of ASH1 mRNA and Ash1p in Yeast}, volume={153}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.153.2.307}, DOI={10.1083/jcb.153.2.307}, number={2}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Long, Roy M. and Gu, Wei and Meng, Xiuhua and Gonsalvez, Graydon and Singer, Robert H. and Chartrand, Pascal}, year={2001}, month=apr, pages={307–318} }

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