A Role for Actin, Cdc1p, and Myo2p in the Inheritance of Late Golgi Elements inSaccharomyces cerevisiae
10.1083/jcb.153.1.47
Crossref journal-article
Rockefeller University Press
The Journal of Cell Biology (291)
Abstract

In Saccharomyces cerevisiae, Golgi elements are present in the bud very early in the cell cycle. We have analyzed this Golgi inheritance process using fluorescence microscopy and genetics. In rapidly growing cells, late Golgi elements show an actin-dependent concentration at sites of polarized growth. Late Golgi elements are apparently transported into the bud along actin cables and are also retained in the bud by a mechanism that may involve actin. A visual screen for mutants defective in the inheritance of late Golgi elements yielded multiple alleles of CDC1. Mutations in CDC1 severely depolarize the actin cytoskeleton, and these mutations prevent late Golgi elements from being retained in the bud. The efficient localization of late Golgi elements to the bud requires the type V myosin Myo2p, further suggesting that actin plays a role in Golgi inheritance. Surprisingly, early and late Golgi elements are inherited by different pathways, with early Golgi elements localizing to the bud in a Cdc1p- and Myo2p-independent manner. We propose that early Golgi elements arise from ER membranes that are present in the bud. These two pathways of Golgi inheritance in S. cerevisiae resemble Golgi inheritance pathways in vertebrate cells.

Bibliography

Rossanese, O. W., Reinke, C. A., Bevis, B. J., Hammond, A. T., Sears, I. B., O’Connor, J., & Glick, B. S. (2001). A Role for Actin, Cdc1p, and Myo2p in the Inheritance of Late Golgi Elements inSaccharomyces cerevisiae. The Journal of Cell Biology, 153(1), 47–62.

Authors 7
  1. Olivia W. Rossanese (first)
  2. Catherine A. Reinke (additional)
  3. Brooke J. Bevis (additional)
  4. Adam T. Hammond (additional)
  5. Irina B. Sears (additional)
  6. James O'Connor (additional)
  7. Benjamin S. Glick (additional)
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Dates
Type When
Created 23 years ago (July 26, 2002, 12:46 p.m.)
Deposited 1 year, 7 months ago (Jan. 4, 2024, 12:13 p.m.)
Indexed 1 month ago (July 20, 2025, 12:21 a.m.)
Issued 24 years, 4 months ago (March 26, 2001)
Published 24 years, 4 months ago (March 26, 2001)
Published Online 24 years, 4 months ago (March 26, 2001)
Published Print 24 years, 4 months ago (April 2, 2001)
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@article{Rossanese_2001, title={A Role for Actin, Cdc1p, and Myo2p in the Inheritance of Late Golgi Elements inSaccharomyces cerevisiae}, volume={153}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.153.1.47}, DOI={10.1083/jcb.153.1.47}, number={1}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Rossanese, Olivia W. and Reinke, Catherine A. and Bevis, Brooke J. and Hammond, Adam T. and Sears, Irina B. and O’Connor, James and Glick, Benjamin S.}, year={2001}, month=mar, pages={47–62} }