DOI: 10.1083/jcb.153.1.229. Actin Bound to the Heterogeneous Nuclear Ribonucleoprotein Hrp36 Is Associated with Balbiani Ring mRNA from the Gene to Polysomes

Actin Bound to the Heterogeneous Nuclear Ribonucleoprotein Hrp36 Is Associated with Balbiani Ring mRNA from the Gene to Polysomes

10.1083/jcb.153.1.229

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

In the salivary glands of the dipteran Chironomus tentans, a specific messenger ribonucleoprotein (mRNP) particle, the Balbiani ring (BR) granule, can be visualized during its assembly on the gene and during its nucleocytoplasmic transport. We now show with immunoelectron microscopy that actin becomes associated with the BR particle concomitantly with transcription and is present in the particle in the nucleoplasm. DNase I affinity chromatography experiments with extracts from tissue culture cells indicate that both nuclear and cytoplasmic actin are bound to the heterogeneous RNP (hnRNP) protein hrp36, but not to the hnRNP proteins hrp23 and hrp45. The interaction is likely to be direct as purified actin binds to recombinant hrp36 in vitro. Furthermore, it is demonstrated by cross linking that nuclear as well as cytoplasmic actin are bound to hrp36 in vivo. It is known that hrp36 is added cotranscriptionally along the BR mRNA molecule and accompanies the RNA through the nuclear pores and into polysomes. We conclude that actin is likely to be bound to the BR transcript via hrp36 during the transfer of the mRNA from the gene all the way into polysomes.

Bibliography

Percipalle, P., Zhao, J., Pope, B., Weeds, A., Lindberg, U., & Daneholt, B. (2001). Actin Bound to the Heterogeneous Nuclear Ribonucleoprotein Hrp36 Is Associated with Balbiani Ring mRNA from the Gene to Polysomes. The Journal of Cell Biology, 153(1), 229â236.

Authors 6

Piergiorgio Percipalle (first)
Jian Zhao (additional)
Brian Pope (additional)
Alan Weeds (additional)
Uno Lindberg (additional)
Bertil Daneholt (additional)

References 18 Referenced 118

10.1101/gad.10.22.2881 / Genes Dev / A protein of the SR family of splicing factors binds extensively to exonic Balbiani ring pre-mRNA and accompanies the RNA from the gene to the nuclear pore by Alzhanova-Ericsson (1996)
10.1016/0022-2836(78)90157-2 / J. Mol. Biol. / The site of the transcription unit in Balbiani ring 2 of Chironomus tentans as derived from analysis of the primary transcript and 75S RNA by Case (1978)
10.1016/0092-8674(77)90152-0 / Cell. / Diffusible and bound actin in nuclei of Xenopus laevis oocytes by Clark (1977)
10.1016/S0092-8674(00)81900-5 / Cell. / A look at messenger RNP moving through the nuclear pore by Daneholt (1997)
10.2307/1538062 / Biol. Bull. / Micrurgical studies on Chironomus salivary gland chromosomes by D'Angelo (1946)
10.1146/annurev.bi.62.070193.001445 / Annu. Rev. Biochem / hnRNP proteins and the biogenesis of mRNA by Dreyfuss (1993)
10.1016/S0955-0674(99)80051-9 / Curr. Opin. Cell Biol / HnRNP complexescomposition, structure, and function by Krecic (1999)
10.1083/jcb.121.2.219 / J. Cell Biol / Association of individual hnRNP proteins with nascent transcripts by Matunis (1993)
10.1016/0092-8674(92)90224-Z / Cell. / Translocation of a specific premessenger ribonucleoprotein particle through the nuclear pore studied with electron microscope tomography by Mehlin (1992)
10.1016/0092-8674(95)90119-1 / Cell. / A nuclear export signal in hnRNP A1a signal-mediated, temperature-dependent nuclear protein export pathway by Michael (1995)
10.1016/0014-4827(85)90179-X / Exp. Cell Res / Association of rapidly-labelled RNAs with actin in nuclear matrix from mouse L5178Y cells by Nakayasu (1985)
10.1016/S0092-8674(00)81666-9 / Cell. / Transport of proteins and RNAs in and out of the nucleus by Nakielny (1999)
10.1016/S0962-8924(99)01713-4 / Trends Cell Biol. / Searching for a function for nuclear actin by Rando (2000)
10.1016/0076-6879(91)96012-G / Methods Enzymol / Affinity chromatography-based purification of profilin:actin by Rozycki (1991)
10.1083/jcb.142.5.1181 / J. Cell Biol / The hrp23 protein in the Balbiani ring pre-mRNP particles is released just before or at the binding of the particles to the nuclear pore complex by Sun (1998)
10.1016/S0092-8674(00)80980-0 / Cell. / A pre-mRNA-binding protein accompanies the RNA from the gene through the nuclear pores and into polysomes by Visa (1996)
10.1128/MCB.16.4.1425 / Mol. Cell. Biol / Identification of two RNA-binding proteins in Balbiani ring premessenger ribonucleoprotein granules and presence of these proteins in specific subsets of heterogeneous nuclear ribonucleoprotein particles by Wurtz (1996)
10.1016/0014-4827(82)90255-5 / Exp. Cell Res. / Chironomus tentans epithelial cell lines sensitive to ecdysteriods, juvenile hormone, insulin and heat shock by Wyss (1982)

Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 12:46 p.m.)
Deposited	2 years, 1 month ago (July 22, 2023, 7:30 a.m.)
Indexed	1 month, 4 weeks ago (July 2, 2025, 3:07 p.m.)
Issued	24 years, 4 months ago (April 2, 2001)
Published	24 years, 4 months ago (April 2, 2001)
Published Online	24 years, 4 months ago (April 2, 2001)
Published Print	24 years, 4 months ago (April 2, 2001)

Funders 0

None

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