DOI: 10.1083/jcb.152.5.1045. The Supramolecular Organization of Fibrillin-Rich Microfibrils

The Supramolecular Organization of Fibrillin-Rich Microfibrils

10.1083/jcb.152.5.1045

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

We propose a new model for the alignment of fibrillin molecules within fibrillin microfibrils. Automated electron tomography was used to generate three-dimensional microfibril reconstructions to 18.6-Å resolution, which revealed many new organizational details of untensioned microfibrils, including heart-shaped beads from which two arms emerge, and interbead diameter variation. Antibody epitope mapping of untensioned microfibrils revealed the juxtaposition of epitopes at the COOH terminus and near the proline-rich region, and of two internal epitopes that would be 42-nm apart in unfolded molecules, which infers intramolecular folding. Colloidal gold binds microfibrils in the absence of antibody. Comparison of colloidal gold and antibody binding sites in untensioned microfibrils and those extended in vitro, and immunofluorescence studies of fibrillin deposition in cell layers, indicate conformation changes and intramolecular folding. Mass mapping shows that, in solution, microfibrils with periodicities of &lt;70 and &gt;140 nm are stable, but periodicities of ∼100 nm are rare. Microfibrils comprise two in-register filaments with a longitudinal symmetry axis, with eight fibrillin molecules in cross section. We present a model of fibrillin alignment that fits all the data and indicates that microfibril extensibility follows conformation-dependent maturation from an initial head-to-tail alignment to a stable approximately one-third staggered arrangement.

Bibliography

Baldock, C., Koster, A. J., Ziese, U., Rock, M. J., Sherratt, M. J., Kadler, K. E., Shuttleworth, C. A., & Kielty, C. M. (2001). The Supramolecular Organization of Fibrillin-Rich Microfibrils. The Journal of Cell Biology, 152(5), 1045â1056.

Authors 8

Clair Baldock (first)
Abraham J. Koster (additional)
Ulrike Ziese (additional)
Matthew J. Rock (additional)
Michael J. Sherratt (additional)
Karl E. Kadler (additional)
C. Adrian Shuttleworth (additional)
Cay M. Kielty (additional)

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 12:46 p.m.)
Deposited	2 years, 1 month ago (July 22, 2023, 7:29 a.m.)
Indexed	1 month, 3 weeks ago (July 8, 2025, 7:29 a.m.)
Issued	24 years, 5 months ago (March 5, 2001)
Published	24 years, 5 months ago (March 5, 2001)
Published Online	24 years, 5 months ago (March 5, 2001)
Published Print	24 years, 5 months ago (March 5, 2001)

Funders 0

None

BibTeX

@article{Baldock_2001, title={The Supramolecular Organization of Fibrillin-Rich Microfibrils}, volume={152}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.152.5.1045}, DOI={10.1083/jcb.152.5.1045}, number={5}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Baldock, Clair and Koster, Abraham J. and Ziese, Ulrike and Rock, Matthew J. and Sherratt, Michael J. and Kadler, Karl E. and Shuttleworth, C. Adrian and Kielty, Cay M.}, year={2001}, month=mar, pages={1045–1056} }

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