DOI: 10.1083/jcb.152.5.1019. ROP Gtpase–Dependent Dynamics of Tip-Localized F-Actin Controls Tip Growth in Pollen Tubes

ROP Gtpase–Dependent Dynamics of Tip-Localized F-Actin Controls Tip Growth in Pollen Tubes

10.1083/jcb.152.5.1019

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

Tip-growing pollen tubes provide a useful model system to study polar growth. Although roles for tip-focused calcium gradient and tip-localized Rho-family GTPase in pollen tube growth is established, the existence and function of tip-localized F-actin have been controversial. Using the green fluorescent protein–tagged actin-binding domain of mouse talin, we found a dynamic form of tip-localized F-actin in tobacco pollen tubes, termed short actin bundles (SABs). The dynamics of SABs during polar growth in pollen tubes is regulated by Rop1At, a Rop GTPase belonging to the Rho family. When overexpressed, Rop1At transformed SAB into a network of fine filaments and induced a transverse actin band behind the tip, leading to depolarized growth. These changes were due to ectopic Rop1At localization to the apical region of the plasma membrane and were suppressed by guanine dissociation inhibitor overexpression, which removed ectopically localized Rop1At. Rop GTPase–activating protein (RopGAP1) overexpression, or Latrunculin B treatments, also recovered normal actin organization and tip growth in Rop1At-overexpressing tubes. Moreover, overexpression of RopGAP1 alone disrupted SABs and inhibited growth. Finally, SAB oscillates and appears at the tip before growth. Together, these results indicate that the dynamics of tip actin are essential for tip growth and provide the first direct evidence to link Rho GTPase to actin organization in controlling cell polarity and polar growth in plants.

Bibliography

Fu, Y., Wu, G., & Yang, Z. (2001). ROP GtpaseâDependent Dynamics of Tip-Localized F-Actin Controls Tip Growth in Pollen Tubes. The Journal of Cell Biology, 152(5), 1019â1032.

Authors 3

Ying Fu (first)
Guang Wu (additional)
Zhenbiao Yang (additional)

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 12:46 p.m.)
Deposited	1 year, 7 months ago (Jan. 4, 2024, 12:13 p.m.)
Indexed	2 weeks, 5 days ago (Aug. 7, 2025, 4:47 p.m.)
Issued	24 years, 5 months ago (March 5, 2001)
Published	24 years, 5 months ago (March 5, 2001)
Published Online	24 years, 5 months ago (March 5, 2001)
Published Print	24 years, 5 months ago (March 5, 2001)

Funders 0

None

BibTeX

@article{Fu_2001, title={ROP Gtpase–Dependent Dynamics of Tip-Localized F-Actin Controls Tip Growth in Pollen Tubes}, volume={152}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.152.5.1019}, DOI={10.1083/jcb.152.5.1019}, number={5}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Fu, Ying and Wu, Guang and Yang, Zhenbiao}, year={2001}, month=mar, pages={1019–1032} }

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