DOI: 10.1083/jcb.152.2.349. The Ndc80p Complex from <i>Saccharomyces cerevisiae</i> Contains Conserved Centromere Components and Has a Function in Chromosome Segregation

The Ndc80p Complex from Saccharomyces cerevisiae Contains Conserved Centromere Components and Has a Function in Chromosome Segregation

10.1083/jcb.152.2.349

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

We have purified a complex from Saccharomyces cerevisiae containing the spindle components Ndc80p, Nuf2p, Spc25p, and Spc24p. Temperature-sensitive mutants in NDC80, SPC25, and SPC24 show defects in chromosome segregation. In spc24-1 cells, green fluorescence protein (GFP)-labeled centromeres fail to split during spindle elongation, and in addition some centromeres may detach from the spindle. Chromatin immunoprecipitation assays show an association of all four components of the complex with the yeast centromere. Homologues of Ndc80p, Nuf2p, and Spc24p were found in Schizosaccharomyces pombe and GFP tagging showed they were located at the centromere. A human homologue of Nuf2p was identified in the expressed sequence tag database. Immunofluorescent staining with anti–human Nuf2p and with anti-HEC, the human homologue of Ndc80p, showed that both proteins are at the centromeres of mitotic HeLa cells. Thus the Ndc80p complex contains centromere-associated components conserved between yeasts and vertebrates.

Bibliography

Wigge, P. A., & Kilmartin, J. V. (2001). The Ndc80p Complex from Saccharomyces cerevisiae Contains Conserved Centromere Components and Has a Function in Chromosome Segregation. The Journal of Cell Biology, 152(2), 349â360.

Authors 2

Philip A. Wigge (first)
John V. Kilmartin (additional)

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 12:46 p.m.)
Deposited	2 years, 1 month ago (July 28, 2023, 10:50 p.m.)
Indexed	20 hours, 22 minutes ago (Sept. 2, 2025, 6:35 a.m.)
Issued	24 years, 7 months ago (Jan. 22, 2001)
Published	24 years, 7 months ago (Jan. 22, 2001)
Published Online	24 years, 7 months ago (Jan. 22, 2001)
Published Print	24 years, 7 months ago (Jan. 22, 2001)

Funders 0

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BibTeX

@article{Wigge_2001, title={The Ndc80p Complex from Saccharomyces cerevisiae Contains Conserved Centromere Components and Has a Function in Chromosome Segregation}, volume={152}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.152.2.349}, DOI={10.1083/jcb.152.2.349}, number={2}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Wigge, Philip A. and Kilmartin, John V.}, year={2001}, month=jan, pages={349–360} }

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