DOI: 10.1083/jcb.151.2.249. Role of the Pi3k Regulatory Subunit in the Control of Actin Organization and Cell Migration

Role of the Pi3k Regulatory Subunit in the Control of Actin Organization and Cell Migration

10.1083/jcb.151.2.249

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

Cell migration represents an important cellular response that utilizes cytoskeletal reorganization as its driving force. Here, we describe a new signaling cascade linking PDGF receptor stimulation to actin rearrangements and cell migration. We demonstrate that PDGF activates Cdc42 and its downstream effector N-WASP to mediate filopodia formation, actin stress fiber disassembly, and a reduction in focal adhesion complexes. Induction of the Cdc42 pathway is independent of phosphoinositide 3-kinase (PI3K) enzymatic activity, but it is dependent on the p85α regulatory subunit of PI3K. Finally, data are provided showing that activation of this pathway is required for PDGF-induced cell migration on collagen. These observations show the essential role of the PI3K regulatory subunit p85α in controlling PDGF receptor–induced cytoskeletal changes and cell migration, illustrating a novel signaling pathway that links receptor stimulation at the cell membrane with actin dynamics.

Bibliography

JimÃ©nez, C., Portela, R. A., Mellado, M., RodrÃguez-Frade, J. M., Collard, J., Serrano, A., MartÃnez-A, C., Avila, J., & Carrera, A. C. (2000). Role of the Pi3k Regulatory Subunit in the Control of Actin Organization and Cell Migration. The Journal of Cell Biology, 151(2), 249â262.

Authors 9

Concepción Jiménez (first)
Rosario Armas Portela (additional)
Mario Mellado (additional)
Jose Miguel Rodríguez-Frade (additional)
John Collard (additional)
Antonio Serrano (additional)
Carlos Martínez-A (additional)
Jesus Avila (additional)
Ana C. Carrera (additional)

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 12:45 p.m.)
Deposited	2 years, 1 month ago (July 28, 2023, 10:36 p.m.)
Indexed	2 weeks, 3 days ago (Aug. 19, 2025, 6:03 a.m.)
Issued	24 years, 10 months ago (Oct. 16, 2000)
Published	24 years, 10 months ago (Oct. 16, 2000)
Published Online	24 years, 10 months ago (Oct. 16, 2000)
Published Print	24 years, 10 months ago (Oct. 16, 2000)

Funders 0

None

BibTeX

@article{Jim_nez_2000, title={Role of the Pi3k Regulatory Subunit in the Control of Actin Organization and Cell Migration}, volume={151}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.151.2.249}, DOI={10.1083/jcb.151.2.249}, number={2}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Jiménez, Concepción and Portela, Rosario Armas and Mellado, Mario and Rodríguez-Frade, Jose Miguel and Collard, John and Serrano, Antonio and Martínez-A, Carlos and Avila, Jesus and Carrera, Ana C.}, year={2000}, month=oct, pages={249–262} }

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