DOI: 10.1083/jcb.150.6.1507. Tor-Mediated Induction of Autophagy via an Apg1 Protein Kinase Complex

Tor-Mediated Induction of Autophagy via an Apg1 Protein Kinase Complex

10.1083/jcb.150.6.1507

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

Autophagy is a membrane trafficking to vacuole/lysosome induced by nutrient starvation. In Saccharomyces cerevisiae, Tor protein, a phosphatidylinositol kinase-related kinase, is involved in the repression of autophagy induction by a largely unknown mechanism. Here, we show that the protein kinase activity of Apg1 is enhanced by starvation or rapamycin treatment. In addition, we have also found that Apg13, which binds to and activates Apg1, is hyperphosphorylated in a Tor-dependent manner, reducing its affinity to Apg1. This Apg1–Apg13 association is required for autophagy, but not for the cytoplasm-to-vacuole targeting (Cvt) pathway, another vesicular transport mechanism in which factors essential for autophagy (Apg proteins) are also employed under vegetative growth conditions. Finally, other Apg1-associating proteins, such as Apg17 and Cvt9, are shown to function specifically in autophagy or the Cvt pathway, respectively, suggesting that the Apg1 complex plays an important role in switching between two distinct vesicular transport systems in a nutrient-dependent manner.

Bibliography

Kamada, Y., Funakoshi, T., Shintani, T., Nagano, K., Ohsumi, M., & Ohsumi, Y. (2000). Tor-Mediated Induction of Autophagy via an Apg1 Protein Kinase Complex. The Journal of Cell Biology, 150(6), 1507â1513.

Authors 6

Yoshiaki Kamada (first)
Tomoko Funakoshi (additional)
Takahiro Shintani (additional)
Kazuya Nagano (additional)
Mariko Ohsumi (additional)
Yoshinori Ohsumi (additional)

References 27 Referenced 918

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10.1083/jcb.139.7.1687 / J. Cell Biol. / Two distinct pathways for targeting proteins from the cytoplasm to the vacuole/lysosome by Baba (1997)
10.1038/45287 / Nature. / The TOR signalling pathway controls nuclear localization of nutrient-regulated transcription factors by Beck (1999)
10.1083/jcb.146.6.1227 / J. Cell Biol. / Starvation induces vacuolar targeting and degradation of the tryptophan permease in yeast by Beck (1999)
10.1101/gad.13.24.3271 / Genes Dev. / The TOR signaling cascade regulates gene expression in response to nutrients by Cardenas (1999)
10.1101/gad.10.15.1904 / Genes Dev / Nutrients, via the Tor proteins, stimulate the association of Tap42 with type 2A phosphatases by Di Como (1996)
10.1016/S0378-1119(97)00031-0 / Gene / Analyses of APG13 gene involved in autophagy in yeast, Saccharomyces cerevisiae by Funakoshi (1997)
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10.1073/pnas.96.26.14866 / Proc. Natl. Acad. Sci. USA. / Rapamycin-modulated transcription defines the subset of nutrient-sensitive signaling pathways directly controlled by the Tor proteins by Hardwick (1999)
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10.1093/emboj/18.10.2782 / EMBO (Eur. Mol. Biol. Organ.) J. / Tor proteins and protein phosphatase 2A reciprocally regulated Tap42 in controlling cell growth in yeast by Jiang (1999)
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10.1016/0092-8674(93)90144-F / Cell. / Target of rapamycin in yeast, TOR2, is an essential phosphatidylinositol kinase homolog required for G1 progression by Kunz (1993)
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10.1006/bbrc.1998.8546 / Biochem. Biophys. Res. Commun. / Identification of mouse ULK1, a novel protein kinase structurally related to C. elegans UNC-51 by Yan (1998)

Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 12:45 p.m.)
Deposited	2 years, 1 month ago (July 28, 2023, 10:24 p.m.)
Indexed	16 hours, 41 minutes ago (Sept. 3, 2025, 6:47 a.m.)
Issued	24 years, 11 months ago (Sept. 18, 2000)
Published	24 years, 11 months ago (Sept. 18, 2000)
Published Online	24 years, 11 months ago (Sept. 18, 2000)
Published Print	24 years, 11 months ago (Sept. 18, 2000)

Funders 0

None

BibTeX

@article{Kamada_2000, title={Tor-Mediated Induction of Autophagy via an Apg1 Protein Kinase Complex}, volume={150}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.150.6.1507}, DOI={10.1083/jcb.150.6.1507}, number={6}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Kamada, Yoshiaki and Funakoshi, Tomoko and Shintani, Takahiro and Nagano, Kazuya and Ohsumi, Mariko and Ohsumi, Yoshinori}, year={2000}, month=sep, pages={1507–1513} }

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