DOI: 10.1083/jcb.150.6.1311. Activation by Cdc42 and Pip2 of Wiskott-Aldrich Syndrome Protein (Wasp) Stimulates Actin Nucleation by Arp2/3 Complex

Activation by Cdc42 and Pip2 of Wiskott-Aldrich Syndrome Protein (Wasp) Stimulates Actin Nucleation by Arp2/3 Complex

10.1083/jcb.150.6.1311

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

We purified native WASp (Wiskott-Aldrich Syndrome protein) from bovine thymus and studied its ability to stimulate actin nucleation by Arp2/3 complex. WASp alone is inactive in the presence or absence of 0.5 μM GTP-Cdc42. Phosphatidylinositol 4,5 bisphosphate (PIP2) micelles allowed WASp to activate actin nucleation by Arp2/3 complex, and this was further enhanced twofold by GTP-Cdc42. Filaments nucleated by Arp2/3 complex and WASp in the presence of PIP2 and Cdc42 concentrated around lipid micelles and vesicles, providing that Cdc42 was GTP-bound and prenylated. Thus, the high concentration of WASp in neutrophils (9 μM) is dependent on interactions with both acidic lipids and GTP-Cdc42 to activate actin nucleation by Arp2/3 complex. The results also suggest that membrane binding increases the local concentrations of Cdc42 and WASp, favoring their interaction.

Bibliography

Higgs, H. N., & Pollard, T. D. (2000). Activation by Cdc42 and Pip2 of Wiskott-Aldrich Syndrome Protein (Wasp) Stimulates Actin Nucleation by Arp2/3 Complex. The Journal of Cell Biology, 150(6), 1311â1320.

Authors 2

Henry N. Higgs (first)
Thomas D. Pollard (additional)

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 12:45 p.m.)
Deposited	2 years, 1 month ago (July 28, 2023, 10:39 p.m.)
Indexed	1 hour, 10 minutes ago (Sept. 2, 2025, 7:36 p.m.)
Issued	24 years, 11 months ago (Sept. 18, 2000)
Published	24 years, 11 months ago (Sept. 18, 2000)
Published Online	24 years, 11 months ago (Sept. 18, 2000)
Published Print	24 years, 11 months ago (Sept. 18, 2000)

Funders 0

None

BibTeX

@article{Higgs_2000, title={Activation by Cdc42 and Pip2 of Wiskott-Aldrich Syndrome Protein (Wasp) Stimulates Actin Nucleation by Arp2/3 Complex}, volume={150}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.150.6.1311}, DOI={10.1083/jcb.150.6.1311}, number={6}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Higgs, Henry N. and Pollard, Thomas D.}, year={2000}, month=sep, pages={1311–1320} }

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