DOI: 10.1083/jcb.150.3.417. Tracking Col1a1 RNA in Osteogenesis Imperfecta

Tracking Col1a1 RNA in Osteogenesis Imperfecta

10.1083/jcb.150.3.417

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

This study illuminates the intra-nuclear fate of COL1A1 RNA in osteogenesis imperfecta (OI) Type I. Patient fibroblasts were shown to carry a heterozygous defect in splicing of intron 26, blocking mRNA export. Both the normal and mutant allele associated with a nuclear RNA track, a localized accumulation of posttranscriptional RNA emanating to one side of the gene. Both tracks had slightly elongated or globular morphology, but mutant tracks were cytologically distinct in that they lacked the normal polar distribution of intron 26. Normal COL1A1 RNA tracks distribute throughout an SC-35 domain, from the gene at the periphery. Normally, almost all 50 COL1A1 introns are spliced at or adjacent to the gene, before mRNA transits thru the domain. Normal COL1A1 transcripts may undergo maturation needed for export within the domain such as removal of a slow-splicing intron (shown for intron 24), after which they may disperse. Splice-defective transcripts still distribute thru the SC-35 domain, moving ∼1–3 μm from the gene. However, microfluorimetric analyses demonstrate mutant transcripts accumulate to abnormal levels within the track and domain. Hence, mutant transcripts initiate transport from the gene, but are impeded in exit from the SC-35 domain. This identifies a previously undefined step in mRNA export, involving movement through an SC-35 domain. A model is presented in which maturation and release for export of COL1A1 mRNA is linked to rapid cycling of metabolic complexes within the splicing factor domain, adjacent to the gene. This paradigm may apply to SC-35 domains more generally, which we suggest may be nucleated at sites of high demand and comprise factors being actively used to facilitate expression of associated loci.

Bibliography

Johnson, C., Primorac, D., McKinstry, M., McNeil, J., Rowe, D., & Lawrence, J. B. (2000). Tracking Col1a1 RNA in Osteogenesis Imperfecta. The Journal of Cell Biology, 150(3), 417â432.

Authors 6

Carol Johnson (first)
Dragan Primorac (additional)
Monique McKinstry (additional)
John McNeil (additional)
David Rowe (additional)
Jeanne Bentley Lawrence (additional)

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Dates

Type	When
Created	23 years ago (July 26, 2002, 12:45 p.m.)
Deposited	2 years ago (July 29, 2023, 2:58 a.m.)
Indexed	4 months, 2 weeks ago (April 5, 2025, 6:22 p.m.)
Issued	25 years ago (Aug. 7, 2000)
Published	25 years ago (Aug. 7, 2000)
Published Online	25 years ago (Aug. 7, 2000)
Published Print	25 years ago (Aug. 7, 2000)

Funders 0

None

BibTeX

@article{Johnson_2000, title={Tracking Col1a1 RNA in Osteogenesis Imperfecta}, volume={150}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.150.3.417}, DOI={10.1083/jcb.150.3.417}, number={3}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Johnson, Carol and Primorac, Dragan and McKinstry, Monique and McNeil, John and Rowe, David and Lawrence, Jeanne Bentley}, year={2000}, month=aug, pages={417–432} }

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  "abstract": "<jats:p>This study illuminates the intra-nuclear fate of COL1A1 RNA in osteogenesis imperfecta (OI) Type I. Patient fibroblasts were shown to carry a heterozygous defect in splicing of intron 26, blocking mRNA export. Both the normal and mutant allele associated with a nuclear RNA track, a localized accumulation of posttranscriptional RNA emanating to one side of the gene. Both tracks had slightly elongated or globular morphology, but mutant tracks were cytologically distinct in that they lacked the normal polar distribution of intron 26. Normal COL1A1 RNA tracks distribute throughout an SC-35 domain, from the gene at the periphery. Normally, almost all 50 COL1A1 introns are spliced at or adjacent to the gene, before mRNA transits thru the domain. Normal COL1A1 transcripts may undergo maturation needed for export within the domain such as removal of a slow-splicing intron (shown for intron 24), after which they may disperse. Splice-defective transcripts still distribute thru the SC-35 domain, moving \u223c1\u20133 \u03bcm from the gene. However, microfluorimetric analyses demonstrate mutant transcripts accumulate to abnormal levels within the track and domain. Hence, mutant transcripts initiate transport from the gene, but are impeded in exit from the SC-35 domain. This identifies a previously undefined step in mRNA export, involving movement through an SC-35 domain. A model is presented in which maturation and release for export of COL1A1 mRNA is linked to rapid cycling of metabolic complexes within the splicing factor domain, adjacent to the gene. This paradigm may apply to SC-35 domains more generally, which we suggest may be nucleated at sites of high demand and comprise factors being actively used to facilitate expression of associated loci.</jats:p>",
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