DOI: 10.1083/jcb.150.3.405. Identification and Characterization of Sa/Scc3p Subunits in the <i>Xenopus</i> and Human Cohesin Complexes

Identification and Characterization of Sa/Scc3p Subunits in the Xenopus and Human Cohesin Complexes

10.1083/jcb.150.3.405

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

A multisubunit protein complex, termed cohesin, plays an essential role in sister chromatid cohesion in yeast and in Xenopus laevis cell-free extracts. We report here that two distinct cohesin complexes exist in Xenopus egg extracts. A 14S complex (x-cohesinSA1) contains XSMC1, XSMC3, XRAD21, and a newly identified subunit, XSA1. In a second 12.5S complex (x-cohesinSA2), XSMC1, XSMC3, and XRAD21 associate with a different subunit, XSA2. Both XSA1 and XSA2 belong to the SA family of mammalian proteins and exhibit similarity to Scc3p, a recently identified component of yeast cohesin. In Xenopus egg extracts, x-cohesinSA1 is predominant, whereas x-cohesinSA2 constitutes only a very minor population. Human cells have a similar pair of cohesin complexes, but the SA2-type is the dominant form in somatic tissue culture cells. Immunolocalization experiments suggest that chromatin association of cohesinSA1 and cohesinSA2 may be differentially regulated. Dissociation of x-cohesinSA1 from chromatin correlates with phosphorylation of XSA1 in the cell-free extracts. Purified cdc2-cyclin B can phosphorylate XSA1 in vitro and reduce the ability of x-cohesinSA1 to bind to DNA or chromatin. These results shed light on the mechanism by which sister chromatid cohesion is partially dissolved in early mitosis, far before the onset of anaphase, in vertebrate cells.

Bibliography

Losada, A., Yokochi, T., Kobayashi, R., & Hirano, T. (2000). Identification and Characterization of Sa/Scc3p Subunits in the Xenopus and Human Cohesin Complexes. The Journal of Cell Biology, 150(3), 405â416.

Authors 4

Ana Losada (first)
Tomoki Yokochi (additional)
Ryuji Kobayashi (additional)
Tatsuya Hirano (additional)

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 12:46 p.m.)
Deposited	2 years, 1 month ago (July 28, 2023, 10:27 p.m.)
Indexed	3 weeks ago (Aug. 7, 2025, 5:01 p.m.)
Issued	25 years ago (Aug. 7, 2000)
Published	25 years ago (Aug. 7, 2000)
Published Online	25 years ago (Aug. 7, 2000)
Published Print	25 years ago (Aug. 7, 2000)

Funders 0

None

BibTeX

@article{Losada_2000, title={Identification and Characterization of Sa/Scc3p Subunits in the Xenopus and Human Cohesin Complexes}, volume={150}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.150.3.405}, DOI={10.1083/jcb.150.3.405}, number={3}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Losada, Ana and Yokochi, Tomoki and Kobayashi, Ryuji and Hirano, Tatsuya}, year={2000}, month=aug, pages={405–416} }

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  "abstract": "<jats:p>A multisubunit protein complex, termed cohesin, plays an essential role in sister chromatid cohesion in yeast and in Xenopus laevis cell-free extracts. We report here that two distinct cohesin complexes exist in Xenopus egg extracts. A 14S complex (x-cohesinSA1) contains XSMC1, XSMC3, XRAD21, and a newly identified subunit, XSA1. In a second 12.5S complex (x-cohesinSA2), XSMC1, XSMC3, and XRAD21 associate with a different subunit, XSA2. Both XSA1 and XSA2 belong to the SA family of mammalian proteins and exhibit similarity to Scc3p, a recently identified component of yeast cohesin. In Xenopus egg extracts, x-cohesinSA1 is predominant, whereas x-cohesinSA2 constitutes only a very minor population. Human cells have a similar pair of cohesin complexes, but the SA2-type is the dominant form in somatic tissue culture cells. Immunolocalization experiments suggest that chromatin association of cohesinSA1 and cohesinSA2 may be differentially regulated. Dissociation of x-cohesinSA1 from chromatin correlates with phosphorylation of XSA1 in the cell-free extracts. Purified cdc2-cyclin B can phosphorylate XSA1 in vitro and reduce the ability of x-cohesinSA1 to bind to DNA or chromatin. These results shed light on the mechanism by which sister chromatid cohesion is partially dissolved in early mitosis, far before the onset of anaphase, in vertebrate cells.</jats:p>",
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