DOI: 10.1083/jcb.150.1.77. The Unfolded Protein Response Regulates Multiple Aspects of Secretory and Membrane Protein Biogenesis and Endoplasmic Reticulum Quality Control

The Unfolded Protein Response Regulates Multiple Aspects of Secretory and Membrane Protein Biogenesis and Endoplasmic Reticulum Quality Control

10.1083/jcb.150.1.77

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

The unfolded protein response (UPR) is an intracellular signaling pathway that relays signals from the lumen of the ER to activate target genes in the nucleus. We devised a genetic screen in the yeast Saccharomyces cerevisiae to isolate mutants that are dependent on activation of the pathway for viability. Using this strategy, we isolated mutants affecting various aspects of ER function, including protein translocation, folding, glycosylation, glycosylphosphatidylinositol modification, and ER-associated protein degradation (ERAD). Extending results gleaned from the genetic studies, we demonstrate that the UPR regulates trafficking of proteins at the translocon to balance the needs of biosynthesis and ERAD. The approach also revealed connections of the UPR to other regulatory pathways. In particular, we identified SON1/RPN4, a recently described transcriptional regulator for genes encoding subunits of the proteasome. Our genetic strategy, therefore, offers a powerful means to provide insight into the physiology of the UPR and to identify novel genes with roles in many aspects of secretory and membrane protein biogenesis.

Bibliography

Ng, D. T. W., Spear, E. D., & Walter, P. (2000). The Unfolded Protein Response Regulates Multiple Aspects of Secretory and Membrane Protein Biogenesis and Endoplasmic Reticulum Quality Control. The Journal of Cell Biology, 150(1), 77â88.

Authors 3

Davis T.W. Ng (first)
Eric D. Spear (additional)
Peter Walter (additional)

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 12:45 p.m.)
Deposited	2 years, 1 month ago (July 22, 2023, 7:07 a.m.)
Indexed	1 month, 2 weeks ago (July 7, 2025, 1:25 a.m.)
Issued	25 years, 1 month ago (July 10, 2000)
Published	25 years, 1 month ago (July 10, 2000)
Published Online	25 years, 1 month ago (July 10, 2000)
Published Print	25 years, 1 month ago (July 10, 2000)

Funders 0

None

BibTeX

@article{Ng_2000, title={The Unfolded Protein Response Regulates Multiple Aspects of Secretory and Membrane Protein Biogenesis and Endoplasmic Reticulum Quality Control}, volume={150}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.150.1.77}, DOI={10.1083/jcb.150.1.77}, number={1}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Ng, Davis T.W. and Spear, Eric D. and Walter, Peter}, year={2000}, month=jul, pages={77–88} }

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