DOI: 10.1083/jcb.150.1.205. Agrin-Induced Acetylcholine Receptor Clustering Is Mediated by the Small Guanosine Triphosphatases Rac and Cdc42

Agrin-Induced Acetylcholine Receptor Clustering Is Mediated by the Small Guanosine Triphosphatases Rac and Cdc42

10.1083/jcb.150.1.205

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

During neuromuscular junction formation, agrin secreted from motor neurons causes muscle cell surface acetylcholine receptors (AChRs) to cluster at synaptic sites by mechanisms that are insufficiently understood. The Rho family of small guanosine triphosphatases (GTPases), including Rac and Cdc42, can mediate focal reorganization of the cell periphery in response to extracellular signals. Here, we investigated the role of Rac and Cdc42 in coupling agrin signaling to AChR clustering. We found that agrin causes marked muscle-specific activation of Rac and Cdc42 in differentiated myotubes, as detected by biochemical measurements. Moreover, this activation is crucial for AChR clustering, since the expression of dominant interfering mutants of either Rac or Cdc42 in myotubes blocks agrin-induced AChR clustering. In contrast, constitutively active Rac and Cdc42 mutants cause AChR to aggregate in the absence of agrin. By indicating that agrin-dependent activation of Rac and Cdc42 constitutes a critical step in the signaling pathway leading to AChR clustering, these findings suggest a novel role for these Rho-GTPases: the coupling of neuronal signaling to a key step in neuromuscular synaptogenesis.

Bibliography

Weston, C., Yee, B., Hod, E., & Prives, J. (2000). Agrin-Induced Acetylcholine Receptor Clustering Is Mediated by the Small Guanosine Triphosphatases Rac and Cdc42. The Journal of Cell Biology, 150(1), 205â212.

Authors 4

Christi Weston (first)
Barry Yee (additional)
Eldad Hod (additional)
Joav Prives (additional)

References 35 Referenced 125

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 12:45 p.m.)
Deposited	2 years, 1 month ago (July 22, 2023, 7:18 a.m.)
Indexed	1 month, 3 weeks ago (July 7, 2025, 2:27 a.m.)
Issued	25 years, 1 month ago (July 10, 2000)
Published	25 years, 1 month ago (July 10, 2000)
Published Online	25 years, 1 month ago (July 10, 2000)
Published Print	25 years, 1 month ago (July 10, 2000)

Funders 0

None

BibTeX

@article{Weston_2000, title={Agrin-Induced Acetylcholine Receptor Clustering Is Mediated by the Small Guanosine Triphosphatases Rac and Cdc42}, volume={150}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.150.1.205}, DOI={10.1083/jcb.150.1.205}, number={1}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Weston, Christi and Yee, Barry and Hod, Eldad and Prives, Joav}, year={2000}, month=jul, pages={205–212} }

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