DOI: 10.1083/jcb.149.4.901. Distinct Membrane Domains on Endosomes in the Recycling Pathway Visualized by Multicolor Imaging of Rab4, Rab5, and Rab11

Distinct Membrane Domains on Endosomes in the Recycling Pathway Visualized by Multicolor Imaging of Rab4, Rab5, and Rab11

10.1083/jcb.149.4.901

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

Two endosome populations involved in recycling of membranes and receptors to the plasma membrane have been described, the early and the recycling endosome. However, this distinction is mainly based on the flow of cargo molecules and the spatial distribution of these membranes within the cell. To get insights into the membrane organization of the recycling pathway, we have studied Rab4, Rab5, and Rab11, three regulatory components of the transport machinery. Following transferrin as cargo molecule and GFP-tagged Rab proteins we could show that cargo moves through distinct domains on endosomes. These domains are occupied by different Rab proteins, revealing compartmentalization within the same continuous membrane. Endosomes are comprised of multiple combinations of Rab4, Rab5, and Rab11 domains that are dynamic but do not significantly intermix over time. Three major populations were observed: one that contains only Rab5, a second with Rab4 and Rab5, and a third containing Rab4 and Rab11. These membrane domains display differential pharmacological sensitivity, reflecting their biochemical and functional diversity. We propose that endosomes are organized as a mosaic of different Rab domains created through the recruitment of specific effector proteins, which cooperatively act to generate a restricted environment on the membrane.

Bibliography

SÃ¶nnichsen, B., De Renzis, S., Nielsen, E., Rietdorf, J., & Zerial, M. (2000). Distinct Membrane Domains on Endosomes in the Recycling Pathway Visualized by Multicolor Imaging of Rab4, Rab5, and Rab11. The Journal of Cell Biology, 149(4), 901â914.

Authors 5

Birte Sönnichsen (first)
Stefano De Renzis (additional)
Erik Nielsen (additional)
Jens Rietdorf (additional)
Marino Zerial (additional)

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Dates

Type	When
Created	23 years ago (July 26, 2002, 12:45 p.m.)
Deposited	2 years, 1 month ago (July 22, 2023, 7:05 a.m.)
Indexed	5 days, 1 hour ago (Aug. 20, 2025, 8:26 a.m.)
Issued	25 years, 3 months ago (May 15, 2000)
Published	25 years, 3 months ago (May 15, 2000)
Published Online	25 years, 3 months ago (May 15, 2000)
Published Print	25 years, 3 months ago (May 15, 2000)

Funders 0

None

BibTeX

@article{S_nnichsen_2000, title={Distinct Membrane Domains on Endosomes in the Recycling Pathway Visualized by Multicolor Imaging of Rab4, Rab5, and Rab11}, volume={149}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.149.4.901}, DOI={10.1083/jcb.149.4.901}, number={4}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Sönnichsen, Birte and De Renzis, Stefano and Nielsen, Erik and Rietdorf, Jens and Zerial, Marino}, year={2000}, month=may, pages={901–914} }

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