DOI: 10.1083/jcb.149.3.575. Association of Yeast RNA Polymerase I with a Nucleolar Substructure Active in Rrna Synthesis and Processing

Association of Yeast RNA Polymerase I with a Nucleolar Substructure Active in Rrna Synthesis and Processing

10.1083/jcb.149.3.575

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

A novel ribonucleoprotein complex enriched in nucleolar proteins was purified from yeast extracts and constituents were identified by mass spectrometry. When isolated from rapidly growing cells, the assembly contained ribonucleic acid (RNA) polymerase (pol) I, and some of its transcription factors like TATA-binding protein (TBP), Rrn3p, Rrn5p, Rrn7p, and Reb1p along with rRNA processing factors, like Nop1p, Cbf5p, Nhp2p, and Rrp5p. The small nucleolar RNAs (snoRNAs) U3, U14, and MRP were also found to be associated with the complex, which supports accurate transcription, termination, and pseudouridylation of rRNA. Formation of the complex did not depend on pol I, and the complex could efficiently recruit exogenous pol I into active ribosomal DNA (rDNA) transcription units. Visualization of the complex by electron microscopy and immunogold labeling revealed a characteristic cluster-forming network of nonuniform size containing nucleolar proteins like Nop1p and Fpr3p and attached pol I. Our results support the idea that a functional nucleolar subdomain formed independently of the state of rDNA transcription may serve as a scaffold for coordinated rRNA synthesis and processing.

Bibliography

Fath, S., Milkereit, P., Podtelejnikov, A. V., Bischler, N., Schultz, P., Bier, M., Mann, M., & Tschochner, H. (2000). Association of Yeast RNA Polymerase I with a Nucleolar Substructure Active in Rrna Synthesis and Processing. The Journal of Cell Biology, 149(3), 575â590.

Authors 8

Stephan Fath (first)
Philipp Milkereit (additional)
Alexandre V. Podtelejnikov (additional)
Nicolas Bischler (additional)
Patrick Schultz (additional)
Mirko Bier (additional)
Matthias Mann (additional)
Herbert Tschochner (additional)

References 80 Referenced 46

10.1128/MCB.19.1.796 / Mol. Cell. Biol. / Histone acetyltransferase and protein kinase activities copurify with a putative RNA polymerase I holoenzyme self-sufficient for promoter-dependent transcription by Albert (1999)
10.1006/jmbi.1998.1693 / J. Mol. Biol. / The role of the 3′ external transcribed spacer in yeast pre-rRNA processing by Allmang (1998)
10.1016/S0092-8674(00)80156-7 / Cell. / The RNA world of the nucleolustwo major families of small RNAs defined by different box elements with related functions by Balakin (1996)
10.1083/jcb.118.6.1297 / J. Cell Biol. / In vitro assembly of prenucleolar bodies in Xenopus egg extract by Bell (1992)
10.1002/j.1460-2075.1992.tb05198.x / EMBO (Eur. Mol. Biol. Organ.) J. / Identification and functional analysis of two U3 binding sites on yeast pre-ribosomal RNA by Beltrame (1992)
10.1083/jcb.127.3.623 / J. Cell Biol. / A novel FK506- and rapamycin-binding protein (FPR3 gene product) in the yeast Saccharomyces cerevisiae is a proline rotamase localized to the nucleolus by Benton (1994)
10.1093/nar/7.1.121 / Nucleic Acids Res. / Pseudouridylation of yeast ribosomal precursor RNA by Brand (1979)
10.1128/MCB.17.10.6175 / Mol. Cell. Biol. / The yeast nucleolar protein Cbf5p is involved in rRNA biosynthesis and interacts genetically with the RNA polymerase I transcription factor RRN3 by Cadwell (1997)
10.1101/gad.12.11.1678 / Genes Dev. / Purification and characterization of the nuclear RNase P holoenzyme complex reveals extensive subunit overlap with RNase MRP by Chamberlain (1998)
10.1074/jbc.271.36.22189 / J. Biol. Chem. / Regulation of the RNA polymerase I and III transcription systems in response to growth conditions by Clarke (1996)
10.1016/0092-8674(92)90232-2 / Cell. / The TATA-binding protein is required for transcription by all three nuclear RNA polymerases in yeast cells by Cormack (1992)
10.1017/S0967199400002884 / Zygote. / Immunocytochemical localization of the nucleolar protein fibrillarin and RNA polymerase I during mouse early embryogenesis by Cuadros-Fernandez (1996)
10.1083/jcb.145.7.1369 / J. Cell Biol. / Assembly of 5S ribosomal RNA is required at a specific step of the pre-rRNA processing pathway by Dechampesme (1999)
10.1073/pnas.94.24.13093 / Proc. Natl. Acad. Sci. USA. / All cyclophilins and FK506 binding proteins are, individually and collectively, dispensable in Saccharomyces cerevisiae by Dolinski (1997)
10.1083/jcb.128.1.15 / J. Cell Biol. / Nuclear spreadsI. Visualization of bipartite ribosomal RNA domains by Garcia Blanco (1995)
10.1002/j.1460-2075.1993.tb05975.x / EMBO (Eur. Mol. Biol. Organ.) J. / Purification of NSP1 reveals complex formation with ‘GLFG’ nucleoporins and a novel nuclear pore protein NIC96 by Grandi (1993)
10.1016/0167-4781(90)90179-6 / Biochim. Biophys. Acta. / Guanosine modifications in runoff transcripts of synthetic transfer RNA-Phe genes microinjected into Xenopus oocytes by Grosjean (1990)
{'key': '2023072902132899700_HarlowandLane1988', 'volume-title': 'AntibodiesA Laboratory Manual', 'author': 'Harlow', 'year': '1988'} / AntibodiesA Laboratory Manual by Harlow (1988)
10.1093/emboj/17.23.7078 / EMBO (Eur. Mol. Biol. Organ.) J. / Nhp2p and Nop10p are essential for the function of H/ACA snoRNPs by Henras (1998)
10.1006/excr.1996.0370 / Exp. Cell Res. / The nucleoskeleton and attached activities by Hozak (1996)
10.1242/jcs.107.2.639 / J. Cell Sci. / Site of transcription of ribosomal RNA and intranucleolar structure in HeLa cells by Hozak (1994)
10.1242/jcs.109.6.1427 / J. Cell Sci. / Active RNA polymerases are localized within discrete transcription “factories' in human nuclei by Iborra (1996)
{'key': '2023072902132899700_JacksonandCook1995', 'first-page': '125', 'article-title': 'The structural basis of nuclear function', 'volume': '162A', 'author': 'Jackson', 'year': '1995', 'journal-title': 'Int. Rev. Cytol.'} / Int. Rev. Cytol. / The structural basis of nuclear function by Jackson (1995)
10.1002/j.1460-2075.1993.tb05747.x / EMBO (Eur. Mol. Biol. Organ.) J. / Visualization of focal sites of transcription within human nuclei by Jackson (1993)
10.1002/(SICI)1097-0231(199608)10:11<1371::AID-RCM682>3.0.CO;2-5 / Rapid Commun. Mass Spectrom. / Delayed extraction improves specificity in database searches by matrix-assisted laser desorption/ionization peptide maps by Jensen (1996)
10.1091/mbc.5.9.955 / Mol. Biol. Cell. / NucleologenesisU3 snRNA-containing prenucleolar bodies move to sites of active pre-rRNA transcription after mitosis by Jimenez Garcia (1994)
10.1083/jcb.133.2.225 / J. Cell Biol. / In vivo evidence that TATA-binding protein/SL1 colocalizes with UBF and RNA polymerase I when rRNA synthesis is either active or inactive by Jordan (1996)
10.1073/pnas.94.25.13458 / Proc. Natl. Acad. Sci. USA. / Histones H3 and H4 are components of upstream activation factor required for the high-level transcription of yeast rDNA by RNA polymerase I by Keener (1997)
10.1074/jbc.273.50.33795 / J. Biol. Chem. / Reconstitution of yeast RNA polymerase I transcription in vitro from purified factors by Keener (1998)
10.1101/gad.8.19.2349 / Genes Dev. / RRN6 and RRN7 encode subunits of a multiprotein complex essential for the initiation of rDNA transcription by RNA polymerase I in Saccharomyces cerevisiae by Keys (1994)
10.1101/gad.10.7.887 / Genes Dev. / Multiprotein transcription factor UAF interacts with the upstream element of the yeast RNA polymerase I promoter and forms a stable preinitiation complex by Keys (1996)
10.1016/S0968-0004(98)01260-2 / TIBS (Trends Biochem. Sci.). / Birth of the snoRNPsthe evolution of the modification-guide snoRNAs by Lafontaine (1998)
10.1101/gad.12.4.527 / Genes Dev. / The box H + ACA snoRNAs carry Cbf5p, the putative rRNA pseudouridine synthase by Lafontaine (1998)
10.1073/pnas.89.15.6958 / Proc. Natl. Acad. Sci. USA. / Ethidium bromide provides a simple tool for identifying genuine DNA-independent protein associations by Lai (1992)
10.1074/jbc.271.35.21062 / J. Biol. Chem. / RRN11 encodes the third subunit of the complex containing Rrn6p and Rrn7p that is essential for the initiation of rDNA transcription by yeast RNA polymerase I by Lalo (1996)
10.1016/S0962-8924(97)01031-3 / Trends Cell Biol. / Cell biology and the genome projects - a concerted strategy for characterizing multiprotein complexes by using mass spectrometry by Lamond (1997)
10.1016/0092-8674(94)90261-5 / Cell. / A model for transcription termination by RNA polymerase I by Lang (1994)
10.1007/BF02510485 / Chromosoma. / Analysis of nucleolar transcription and processing domains and pre-rRNA movements by in situ hybridization by Lazdins (1997)
10.1007/BF02510491 / Chromosoma. / Ultrastructural changes in the Schizosaccharomyces pombe nucleolus following the disruption of the gar2+ gene, which encodes a nucleolar protein structurally related to nucleolin by Leger-Silvestre (1997)
10.1007/s004120050357 / Chromsoma. / Functional compartmentalization of the nucleus in the budding yeast Saccharomyces cerevisiae by Leger-Silvestre (1999)
10.1128/MCB.16.11.6436 / Mol. Cell. Biol. / A novel 66-kilodalton protein complexes with Rrn6, Rrn7, and TATA-binding protein to promote polymerase I transcription initiation in Saccharomyces cerevisiae by Lin (1996)
10.1093/emboj/17.13.3692 / EMBO (Eur. Mol. Biol. Organ.) J. / A specialized form of RNA polymerase I, essential for initiation and growth dependent regulation of rRNA synthesis, is disrupted during transcription by Milkereit (1998)
10.1515/bchm.1997.378.12.1433 / Biol. Chem. / Resolution of RNA polymerase I into dimers and monomers and their function in transcription by Milkereit (1997)
10.1016/S0092-8674(00)80432-8 / Cell. / The exosomea conserved eukaryotic RNA processing complex containing multiple 3′→5′ exoribonucleases by Mitchell (1997)
10.1007/BF02510488 / Chromosoma. / U14 small nucleolar RNA makes multiple contacts with the pre-ribosomal RNA by Morrissey (1997)
10.1007/s004180050208 / Histochem. Cell Biol. / Ribosomal gene transcription is organized in foci within nucleolar components by Mosgoeller (1998)
10.1101/gad.7.8.1609 / Genes Dev. / The terminal balls characteristic of eukaryotic rRNA transcription units in chromatin spreads are rRNA processing complexes by Mougey (1993)
10.1093/nar/17.23.9661 / Nucleic Acids Res. / Linker scanning of the yeast RNA polymerase I promoter by Musters (1989)
10.1073/pnas.88.9.3962 / Proc. Natl. Acad. Sci. USA. / Synthesis of large rRNAs by RNA polymerase II in mutants of Saccharomyces cerevisiae defective in RNA polymerase I by Nogi (1991)
{'key': '2023072902132899700_Oakesetal1993', 'first-page': '2441', 'article-title': 'Structural alterations of the nucleolus in mutants of Saccharomyces cerevisiae defective in RNA polymerase I', 'volume': '13', 'author': 'Oakes', 'year': '1993', 'journal-title': 'Mol. Cell. Biol.'} / Mol. Cell. Biol. / Structural alterations of the nucleolus in mutants of Saccharomyces cerevisiae defective in RNA polymerase I by Oakes (1993)
10.1083/jcb.143.1.23 / J. Cell Biol. / Mutational analysis of the structure and localization of the nucleolus in the yeast Saccharomyces cerevisiae by Oakes (1998)
10.1093/emboj/18.8.2241 / EMBO (Eur. Mol. Biol. Organ.) J. / Regional specialization in human nucleivisualization of discrete sites of transcription by RNA polymerase III by Pombo (1999)
10.1074/jbc.270.11.6205 / J. Biol. Chem. / Characterization of the components of reconstituted Saccharomyces cerevisiae RNA polymerase I transcription complexes by Riggs (1995)
10.1073/pnas.94.22.11869 / Proc. Natl. Acad. Sci. USA. / Extensive purification of a putative RNA polymerase I holoenzyme from plants that accurately initiates rRNA gene transcription in vitro by Saez-Vasquez (1997)
{'key': '2023072902132899700_Sambrooketal1989', 'volume-title': 'Molecular CloningA Laboratory Manual', 'author': 'Sambrook', 'year': '1989'} / Molecular CloningA Laboratory Manual by Sambrook (1989)
10.1016/S0955-0674(99)80054-4 / Curr. Opin. Cell Biol. / Structure and function of the nucleolus by Scheer (1999)
10.1016/0092-8674(92)90233-3 / Cell. / Variants of the TATA-binding protein can distinguish subsets of RNA polymerase I, II, and III promoters by Schultz (1992)
10.1016/S0022-2836(05)80326-2 / J. Mol. Biol. / Structural study of the yeast RNA polymerase A. Electron microscopy of lipid-bound molecules and two-dimensional crystals by Schultz (1990)
10.1006/jmbi.1997.1434 / J. Mol. Biol. / Mammalian RNA polymerase I exists as a holoenzyme with associated basal transcription factors by Seither (1998)
10.1073/pnas.93.25.14440 / Proc. Natl. Acad. Sci. USA. / Linking genome and proteome by mass spectrometrylarge-scale identification of yeast proteins from two dimensional gels by Shevchenko (1996)
10.1021/ac950914h / Anal. Chem. / Mass spectrometric sequencing of proteins silver-stained polyacrylamide gels by Shevchenko (1996)
10.1002/j.1460-2075.1996.tb00580.x / EMBO (Eur. Mol. Biol. Organ.) J. / Nuclear pore proteins are involved in the biogenesis of functional tRNA by Simos (1996)
10.1016/S0092-8674(00)80247-0 / Cell. / Sno storm in the nucleolusnew roles for myriad small RNPs by Smith (1997)
10.1101/gad.10.20.2551 / Genes Dev. / The role of TBP in rDNA transcription by RNA polymerase I in Saccharomyces cerevisiaeTBP is required for upstream activation factor dependent recruitment of core factor by Steffan (1996)
10.1128/MCB.18.7.3752 / Mol. Cell. Biol. / Interaction of TATA-binding protein with upstream activation factor is required for activated transcription of ribosomal DNA by RNA polymerase I in Saccharomyces cerevisiae in vivo by Steffan (1998)
{'key': '2023072902132899700_StewartandRoeder1989', 'first-page': '3464', 'article-title': 'Transcription by RNA polymerase I stimulates mitotic recombination in Saccharomyces cerevisiae', 'volume': '9', 'author': 'Stewart', 'year': '1989', 'journal-title': 'Mol. Cell. Biol.'} / Mol. Cell. Biol. / Transcription by RNA polymerase I stimulates mitotic recombination in Saccharomyces cerevisiae by Stewart (1989)
10.1002/j.1460-2075.1994.tb06786.x / EMBO (Eur. Mol. Biol. Organ.) J. / Intron-dependent formation of pseudouridines in the anticodon of Saccharomyces cerevisiae minor tRNA(Ile) by Szweykowska Kulinska (1994)
10.1016/S0955-0674(97)80005-1 / Curr. Opin. Cell Biol. / Function and synthesis of small nucleolar RNAs by Tollervey (1997)
10.1073/pnas.93.23.12914 / Proc. Natl. Acad. Sci. USA. / A novel RNA polymerase I-dependent RNase activity that shortens nascent transcripts from the 3′ end by Tschochner (1996)
10.1016/S0014-5793(97)00636-4 / FEBS Lett. / RNA polymerase I from S. cerevisiae depends on an additional factor to release terminated transcripts from the template by Tschochner (1997)
10.1016/0022-2836(88)90382-8 / J. Mol. Biol. / Rapid initial cleavage of nascent pre-rRNA transcripts in yeast by Veinot Drebot (1988)
10.1002/yea.320111607 / Yeast. / Processing of pre-ribosomal RNA in Saccharomyces cerevisiae by Venema (1995)
10.1002/j.1460-2075.1996.tb00954.x / EMBO (Eur. Mol. Biol. Organ.) J. / RRP5 is required for formation of both 18S and 5.8S rRNA in yeast by Venema (1996)
10.1083/jcb.142.5.1167 / J. Cell Biol. / Presence of pre-rRNAs before activation of polymerase I transcription in the building process of nucleoli during early development of Xenopus laevis by Verheggen (1998)
10.1021/ac00091a044 / Anal. Chem. / Improved resolution and very high sensitivity in MALDI TOF of matrix surfaces made by fast evaporation by Vorm (1994)
10.1083/jcb.122.2.283 / J. Cell Biol. / Fluorescent labeling of nascent RNA reveals transcription by RNA polymerase II in domains scattered throughout the nucleus by Wansink (1993)
10.1017/S1355838298980761 / RNA. / Cbf5p, a potential pseudouridine synthase, and Nhp2p, a putative RNA-binding protein, are present together with Gar1p in all H BOX/ACA-motif snoRNPs and constitute a common bipartite structure by Watkins (1998)
10.1006/excr.1996.0287 / Exp. Cell Res. / The transcription unit of ribosomal genes is attached to the nuclear skeleton by Weipoltshammer (1996)
10.1002/j.1460-2075.1996.tb00770.x / EMBO (Eur. Mol. Biol. Organ.) J. / RRN3 gene of Saccharomyces cerevisiae encodes an essential RNA polymerase I transcription factor which interacts with the polymerase independently of DNA template by Yamamoto (1996)
10.1007/BF02510478 / Chromosoma. / Experimental induction of prenucleolar bodies (PNBs) in interphase cellsinterphase PNBs show similar characteristics as those typically observed at telophase of mitosis in untreated cells by Zatsepina (1997)

Dates

Type	When
Created	23 years ago (July 26, 2002, 12:45 p.m.)
Deposited	2 years ago (July 28, 2023, 10:14 p.m.)
Indexed	21 hours, 25 minutes ago (Aug. 21, 2025, 1:19 p.m.)
Issued	25 years, 3 months ago (May 1, 2000)
Published	25 years, 3 months ago (May 1, 2000)
Published Online	25 years, 3 months ago (May 1, 2000)
Published Print	25 years, 3 months ago (May 1, 2000)

Funders 0

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BibTeX

@article{Fath_2000, title={Association of Yeast RNA Polymerase I with a Nucleolar Substructure Active in Rrna Synthesis and Processing}, volume={149}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.149.3.575}, DOI={10.1083/jcb.149.3.575}, number={3}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Fath, Stephan and Milkereit, Philipp and Podtelejnikov, Alexandre V. and Bischler, Nicolas and Schultz, Patrick and Bier, Mirko and Mann, Matthias and Tschochner, Herbert}, year={2000}, month=may, pages={575–590} }

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      "key": "2023072902132899700_Albertetal1999",
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      "first-page": "796",
      "DOI": "10.1128/MCB.19.1.796",
      "article-title": "Histone acetyltransferase and protein kinase activities copurify with a putative RNA polymerase I holoenzyme self-sufficient for promoter-dependent transcription",
      "volume": "19",
      "author": "Albert",
      "year": "1999",
      "journal-title": "Mol. Cell. Biol."
    },
    {
      "key": "2023072902132899700_AllmangandTollervey1998",
      "doi-asserted-by": "crossref",
      "first-page": "67",
      "DOI": "10.1006/jmbi.1998.1693",
      "article-title": "The role of the 3\u2032 external transcribed spacer in yeast pre-rRNA processing",
      "volume": "278",
      "author": "Allmang",
      "year": "1998",
      "journal-title": "J. Mol. Biol."
    },
    {
      "key": "2023072902132899700_Balakinetal1996",
      "doi-asserted-by": "crossref",
      "first-page": "823",
      "DOI": "10.1016/S0092-8674(00)80156-7",
      "article-title": "The RNA world of the nucleolustwo major families of small RNAs defined by different box elements with related functions",
      "volume": "86",
      "author": "Balakin",
      "year": "1996",
      "journal-title": "Cell."
    },
    {
      "key": "2023072902132899700_Belletal1992",
      "doi-asserted-by": "crossref",
      "first-page": "1297",
      "DOI": "10.1083/jcb.118.6.1297",
      "article-title": "In vitro assembly of prenucleolar bodies in Xenopus egg extract",
      "volume": "118",
      "author": "Bell",
      "year": "1992",
      "journal-title": "J. Cell Biol."
    },
    {
      "key": "2023072902132899700_BeltrameandTollervey1992",
      "doi-asserted-by": "crossref",
      "first-page": "1531",
      "DOI": "10.1002/j.1460-2075.1992.tb05198.x",
      "article-title": "Identification and functional analysis of two U3 binding sites on yeast pre-ribosomal RNA",
      "volume": "11",
      "author": "Beltrame",
      "year": "1992",
      "journal-title": "EMBO (Eur. Mol. Biol. Organ.) J."
    },
    {
      "key": "2023072902132899700_Bentonetal1994",
      "doi-asserted-by": "crossref",
      "first-page": "623",
      "DOI": "10.1083/jcb.127.3.623",
      "article-title": "A novel FK506- and rapamycin-binding protein (FPR3 gene product) in the yeast Saccharomyces cerevisiae is a proline rotamase localized to the nucleolus",
      "volume": "127",
      "author": "Benton",
      "year": "1994",
      "journal-title": "J. Cell Biol."
    },
    {
      "key": "2023072902132899700_Brandetal1979",
      "doi-asserted-by": "crossref",
      "first-page": "121",
      "DOI": "10.1093/nar/7.1.121",
      "article-title": "Pseudouridylation of yeast ribosomal precursor RNA",
      "volume": "7",
      "author": "Brand",
      "year": "1979",
      "journal-title": "Nucleic Acids Res."
    },
    {
      "key": "2023072902132899700_Cadwelletal1997",
      "doi-asserted-by": "crossref",
      "first-page": "6175",
      "DOI": "10.1128/MCB.17.10.6175",
      "article-title": "The yeast nucleolar protein Cbf5p is involved in rRNA biosynthesis and interacts genetically with the RNA polymerase I transcription factor RRN3",
      "volume": "17",
      "author": "Cadwell",
      "year": "1997",
      "journal-title": "Mol. Cell. Biol."
    },
    {
      "key": "2023072902132899700_Chamberlainetal1998",
      "doi-asserted-by": "crossref",
      "first-page": "1678",
      "DOI": "10.1101/gad.12.11.1678",
      "article-title": "Purification and characterization of the nuclear RNase P holoenzyme complex reveals extensive subunit overlap with RNase MRP",
      "volume": "12",
      "author": "Chamberlain",
      "year": "1998",
      "journal-title": "Genes Dev."
    },
    {
      "key": "2023072902132899700_Clarkeetal1996",
      "doi-asserted-by": "crossref",
      "first-page": "22189",
      "DOI": "10.1074/jbc.271.36.22189",
      "article-title": "Regulation of the RNA polymerase I and III transcription systems in response to growth conditions",
      "volume": "271",
      "author": "Clarke",
      "year": "1996",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "2023072902132899700_CormackandStruhl1992",
      "doi-asserted-by": "crossref",
      "first-page": "685",
      "DOI": "10.1016/0092-8674(92)90232-2",
      "article-title": "The TATA-binding protein is required for transcription by all three nuclear RNA polymerases in yeast cells",
      "volume": "69",
      "author": "Cormack",
      "year": "1992",
      "journal-title": "Cell."
    },
    {
      "key": "2023072902132899700_Cuadros-FernandezandEsponada1996",
      "doi-asserted-by": "crossref",
      "first-page": "49",
      "DOI": "10.1017/S0967199400002884",
      "article-title": "Immunocytochemical localization of the nucleolar protein fibrillarin and RNA polymerase I during mouse early embryogenesis",
      "volume": "4",
      "author": "Cuadros-Fernandez",
      "year": "1996",
      "journal-title": "Zygote."
    },
    {
      "key": "2023072902132899700_Dechampesmeetal1999",
      "doi-asserted-by": "crossref",
      "first-page": "1369",
      "DOI": "10.1083/jcb.145.7.1369",
      "article-title": "Assembly of 5S ribosomal RNA is required at a specific step of the pre-rRNA processing pathway",
      "volume": "145",
      "author": "Dechampesme",
      "year": "1999",
      "journal-title": "J. Cell Biol."
    },
    {
      "key": "2023072902132899700_Dolinskietal1997",
      "doi-asserted-by": "crossref",
      "first-page": "13093",
      "DOI": "10.1073/pnas.94.24.13093",
      "article-title": "All cyclophilins and FK506 binding proteins are, individually and collectively, dispensable in Saccharomyces cerevisiae",
      "volume": "94",
      "author": "Dolinski",
      "year": "1997",
      "journal-title": "Proc. Natl. Acad. Sci. USA."
    },
    {
      "key": "2023072902132899700_GarciaBlancoetal1995",
      "doi-asserted-by": "crossref",
      "first-page": "15",
      "DOI": "10.1083/jcb.128.1.15",
      "article-title": "Nuclear spreadsI. Visualization of bipartite ribosomal RNA domains",
      "volume": "128",
      "author": "Garcia Blanco",
      "year": "1995",
      "journal-title": "J. Cell Biol."
    },
    {
      "key": "2023072902132899700_Grandietal1993",
      "doi-asserted-by": "crossref",
      "first-page": "3061",
      "DOI": "10.1002/j.1460-2075.1993.tb05975.x",
      "article-title": "Purification of NSP1 reveals complex formation with \u2018GLFG\u2019 nucleoporins and a novel nuclear pore protein NIC96",
      "volume": "12",
      "author": "Grandi",
      "year": "1993",
      "journal-title": "EMBO (Eur. Mol. Biol. Organ.) J."
    },
    {
      "key": "2023072902132899700_Grosjeanetal1990",
      "doi-asserted-by": "crossref",
      "first-page": "267",
      "DOI": "10.1016/0167-4781(90)90179-6",
      "article-title": "Guanosine modifications in runoff transcripts of synthetic transfer RNA-Phe genes microinjected into Xenopus oocytes",
      "volume": "1050",
      "author": "Grosjean",
      "year": "1990",
      "journal-title": "Biochim. Biophys. Acta."
    },
    {
      "key": "2023072902132899700_HarlowandLane1988",
      "volume-title": "AntibodiesA Laboratory Manual",
      "author": "Harlow",
      "year": "1988"
    },
    {
      "key": "2023072902132899700_Henrasetal1998",
      "doi-asserted-by": "crossref",
      "first-page": "7078",
      "DOI": "10.1093/emboj/17.23.7078",
      "article-title": "Nhp2p and Nop10p are essential for the function of H/ACA snoRNPs",
      "volume": "17",
      "author": "Henras",
      "year": "1998",
      "journal-title": "EMBO (Eur. Mol. Biol. Organ.) J."
    },
    {
      "key": "2023072902132899700_Hozak1996",
      "doi-asserted-by": "crossref",
      "first-page": "267",
      "DOI": "10.1006/excr.1996.0370",
      "article-title": "The nucleoskeleton and attached activities",
      "volume": "229",
      "author": "Hozak",
      "year": "1996",
      "journal-title": "Exp. Cell Res."
    },
    {
      "key": "2023072902132899700_Hozaketal1994",
      "doi-asserted-by": "crossref",
      "first-page": "639",
      "DOI": "10.1242/jcs.107.2.639",
      "article-title": "Site of transcription of ribosomal RNA and intranucleolar structure in HeLa cells",
      "volume": "107",
      "author": "Hozak",
      "year": "1994",
      "journal-title": "J. Cell Sci."
    },
    {
      "key": "2023072902132899700_Iborraetal1996",
      "doi-asserted-by": "crossref",
      "first-page": "1427",
      "DOI": "10.1242/jcs.109.6.1427",
      "article-title": "Active RNA polymerases are localized within discrete transcription \u201cfactories' in human nuclei",
      "volume": "109",
      "author": "Iborra",
      "year": "1996",
      "journal-title": "J. Cell Sci."
    },
    {
      "key": "2023072902132899700_JacksonandCook1995",
      "first-page": "125",
      "article-title": "The structural basis of nuclear function",
      "volume": "162A",
      "author": "Jackson",
      "year": "1995",
      "journal-title": "Int. Rev. Cytol."
    },
    {
      "key": "2023072902132899700_Jacksonetal1993",
      "doi-asserted-by": "crossref",
      "first-page": "1059",
      "DOI": "10.1002/j.1460-2075.1993.tb05747.x",
      "article-title": "Visualization of focal sites of transcription within human nuclei",
      "volume": "12",
      "author": "Jackson",
      "year": "1993",
      "journal-title": "EMBO (Eur. Mol. Biol. Organ.) J."
    },
    {
      "key": "2023072902132899700_Jensenetal1996",
      "doi-asserted-by": "crossref",
      "first-page": "1371",
      "DOI": "10.1002/(SICI)1097-0231(199608)10:11<1371::AID-RCM682>3.0.CO;2-5",
      "article-title": "Delayed extraction improves specificity in database searches by matrix-assisted laser desorption/ionization peptide maps",
      "volume": "10",
      "author": "Jensen",
      "year": "1996",
      "journal-title": "Rapid Commun. Mass Spectrom."
    },
    {
      "key": "2023072902132899700_JimenezGarciaetal1994",
      "doi-asserted-by": "crossref",
      "first-page": "955",
      "DOI": "10.1091/mbc.5.9.955",
      "article-title": "NucleologenesisU3 snRNA-containing prenucleolar bodies move to sites of active pre-rRNA transcription after mitosis",
      "volume": "5",
      "author": "Jimenez Garcia",
      "year": "1994",
      "journal-title": "Mol. Biol. Cell."
    },
    {
      "key": "2023072902132899700_Jordanetal1996",
      "doi-asserted-by": "crossref",
      "first-page": "225",
      "DOI": "10.1083/jcb.133.2.225",
      "article-title": "In vivo evidence that TATA-binding protein/SL1 colocalizes with UBF and RNA polymerase I when rRNA synthesis is either active or inactive",
      "volume": "133",
      "author": "Jordan",
      "year": "1996",
      "journal-title": "J. Cell Biol."
    },
    {
      "key": "2023072902132899700_Keeneretal1997",
      "doi-asserted-by": "crossref",
      "first-page": "13458",
      "DOI": "10.1073/pnas.94.25.13458",
      "article-title": "Histones H3 and H4 are components of upstream activation factor required for the high-level transcription of yeast rDNA by RNA polymerase I",
      "volume": "94",
      "author": "Keener",
      "year": "1997",
      "journal-title": "Proc. Natl. Acad. Sci. USA."
    },
    {
      "key": "2023072902132899700_Keeneretal1998",
      "doi-asserted-by": "crossref",
      "first-page": "33795",
      "DOI": "10.1074/jbc.273.50.33795",
      "article-title": "Reconstitution of yeast RNA polymerase I transcription in vitro from purified factors",
      "volume": "273",
      "author": "Keener",
      "year": "1998",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "2023072902132899700_Keysetal1994",
      "doi-asserted-by": "crossref",
      "first-page": "2349",
      "DOI": "10.1101/gad.8.19.2349",
      "article-title": "RRN6 and RRN7 encode subunits of a multiprotein complex essential for the initiation of rDNA transcription by RNA polymerase I in Saccharomyces cerevisiae",
      "volume": "8",
      "author": "Keys",
      "year": "1994",
      "journal-title": "Genes Dev."
    },
    {
      "key": "2023072902132899700_Keysetal1996",
      "doi-asserted-by": "crossref",
      "first-page": "887",
      "DOI": "10.1101/gad.10.7.887",
      "article-title": "Multiprotein transcription factor UAF interacts with the upstream element of the yeast RNA polymerase I promoter and forms a stable preinitiation complex",
      "volume": "10",
      "author": "Keys",
      "year": "1996",
      "journal-title": "Genes Dev."
    },
    {
      "key": "2023072902132899700_LafontaineandTollervey1998",
      "doi-asserted-by": "crossref",
      "first-page": "383",
      "DOI": "10.1016/S0968-0004(98)01260-2",
      "article-title": "Birth of the snoRNPsthe evolution of the modification-guide snoRNAs",
      "volume": "23",
      "author": "Lafontaine",
      "year": "1998",
      "journal-title": "TIBS (Trends Biochem. Sci.)."
    },
    {
      "key": "2023072902132899700_Lafontaineetal1998",
      "doi-asserted-by": "crossref",
      "first-page": "527",
      "DOI": "10.1101/gad.12.4.527",
      "article-title": "The box H + ACA snoRNAs carry Cbf5p, the putative rRNA pseudouridine synthase",
      "volume": "12",
      "author": "Lafontaine",
      "year": "1998",
      "journal-title": "Genes Dev."
    },
    {
      "key": "2023072902132899700_LaiandHerr1992",
      "doi-asserted-by": "crossref",
      "first-page": "6958",
      "DOI": "10.1073/pnas.89.15.6958",
      "article-title": "Ethidium bromide provides a simple tool for identifying genuine DNA-independent protein associations",
      "volume": "89",
      "author": "Lai",
      "year": "1992",
      "journal-title": "Proc. Natl. Acad. Sci. USA."
    },
    {
      "key": "2023072902132899700_Laloetal1996",
      "doi-asserted-by": "crossref",
      "first-page": "21062",
      "DOI": "10.1074/jbc.271.35.21062",
      "article-title": "RRN11 encodes the third subunit of the complex containing Rrn6p and Rrn7p that is essential for the initiation of rDNA transcription by yeast RNA polymerase I",
      "volume": "271",
      "author": "Lalo",
      "year": "1996",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "2023072902132899700_LamondandMann1997",
      "doi-asserted-by": "crossref",
      "first-page": "139",
      "DOI": "10.1016/S0962-8924(97)01031-3",
      "article-title": "Cell biology and the genome projects - a concerted strategy for characterizing multiprotein complexes by using mass spectrometry",
      "volume": "7",
      "author": "Lamond",
      "year": "1997",
      "journal-title": "Trends Cell Biol."
    },
    {
      "key": "2023072902132899700_Langetal1994",
      "doi-asserted-by": "crossref",
      "first-page": "527",
      "DOI": "10.1016/0092-8674(94)90261-5",
      "article-title": "A model for transcription termination by RNA polymerase I",
      "volume": "79",
      "author": "Lang",
      "year": "1994",
      "journal-title": "Cell."
    },
    {
      "key": "2023072902132899700_Lazdinsetal1997",
      "doi-asserted-by": "crossref",
      "first-page": "481",
      "DOI": "10.1007/BF02510485",
      "article-title": "Analysis of nucleolar transcription and processing domains and pre-rRNA movements by in situ hybridization",
      "volume": "105",
      "author": "Lazdins",
      "year": "1997",
      "journal-title": "Chromosoma."
    },
    {
      "key": "2023072902132899700_Leger-Silvestreetal1997",
      "doi-asserted-by": "crossref",
      "first-page": "542",
      "DOI": "10.1007/BF02510491",
      "article-title": "Ultrastructural changes in the Schizosaccharomyces pombe nucleolus following the disruption of the gar2+ gene, which encodes a nucleolar protein structurally related to nucleolin",
      "volume": "105",
      "author": "Leger-Silvestre",
      "year": "1997",
      "journal-title": "Chromosoma."
    },
    {
      "key": "2023072902132899700_Leger-Silvestreetal1999",
      "doi-asserted-by": "crossref",
      "first-page": "103",
      "DOI": "10.1007/s004120050357",
      "article-title": "Functional compartmentalization of the nucleus in the budding yeast Saccharomyces cerevisiae",
      "volume": "108",
      "author": "Leger-Silvestre",
      "year": "1999",
      "journal-title": "Chromsoma."
    },
    {
      "key": "2023072902132899700_Linetal1996",
      "doi-asserted-by": "crossref",
      "first-page": "6436",
      "DOI": "10.1128/MCB.16.11.6436",
      "article-title": "A novel 66-kilodalton protein complexes with Rrn6, Rrn7, and TATA-binding protein to promote polymerase I transcription initiation in Saccharomyces cerevisiae",
      "volume": "16",
      "author": "Lin",
      "year": "1996",
      "journal-title": "Mol. Cell. Biol."
    },
    {
      "key": "2023072902132899700_MilkereitandTschochner1998",
      "doi-asserted-by": "crossref",
      "first-page": "3692",
      "DOI": "10.1093/emboj/17.13.3692",
      "article-title": "A specialized form of RNA polymerase I, essential for initiation and growth dependent regulation of rRNA synthesis, is disrupted during transcription",
      "volume": "17",
      "author": "Milkereit",
      "year": "1998",
      "journal-title": "EMBO (Eur. Mol. Biol. Organ.) J."
    },
    {
      "key": "2023072902132899700_Milkereitetal1997",
      "doi-asserted-by": "crossref",
      "first-page": "1433",
      "DOI": "10.1515/bchm.1997.378.12.1433",
      "article-title": "Resolution of RNA polymerase I into dimers and monomers and their function in transcription",
      "volume": "378",
      "author": "Milkereit",
      "year": "1997",
      "journal-title": "Biol. Chem."
    },
    {
      "key": "2023072902132899700_Mitchelletal1997",
      "doi-asserted-by": "crossref",
      "first-page": "457",
      "DOI": "10.1016/S0092-8674(00)80432-8",
      "article-title": "The exosomea conserved eukaryotic RNA processing complex containing multiple 3\u2032\u21925\u2032 exoribonucleases",
      "volume": "91",
      "author": "Mitchell",
      "year": "1997",
      "journal-title": "Cell."
    },
    {
      "key": "2023072902132899700_MorrisseyandTollervey1997",
      "doi-asserted-by": "crossref",
      "first-page": "515",
      "DOI": "10.1007/BF02510488",
      "article-title": "U14 small nucleolar RNA makes multiple contacts with the pre-ribosomal RNA",
      "volume": "105",
      "author": "Morrissey",
      "year": "1997",
      "journal-title": "Chromosoma."
    },
    {
      "key": "2023072902132899700_Mosgoelleretal1998",
      "doi-asserted-by": "crossref",
      "first-page": "111",
      "DOI": "10.1007/s004180050208",
      "article-title": "Ribosomal gene transcription is organized in foci within nucleolar components",
      "volume": "109",
      "author": "Mosgoeller",
      "year": "1998",
      "journal-title": "Histochem. Cell Biol."
    },
    {
      "key": "2023072902132899700_Mougeyetal1993",
      "doi-asserted-by": "crossref",
      "first-page": "1609",
      "DOI": "10.1101/gad.7.8.1609",
      "article-title": "The terminal balls characteristic of eukaryotic rRNA transcription units in chromatin spreads are rRNA processing complexes",
      "volume": "7",
      "author": "Mougey",
      "year": "1993",
      "journal-title": "Genes Dev."
    },
    {
      "key": "2023072902132899700_Mustersetal1989a",
      "doi-asserted-by": "crossref",
      "first-page": "9661",
      "DOI": "10.1093/nar/17.23.9661",
      "article-title": "Linker scanning of the yeast RNA polymerase I promoter",
      "volume": "17",
      "author": "Musters",
      "year": "1989",
      "journal-title": "Nucleic Acids Res."
    },
    {
      "key": "2023072902132899700_Nogietal1991",
      "doi-asserted-by": "crossref",
      "first-page": "3962",
      "DOI": "10.1073/pnas.88.9.3962",
      "article-title": "Synthesis of large rRNAs by RNA polymerase II in mutants of Saccharomyces cerevisiae defective in RNA polymerase I",
      "volume": "88",
      "author": "Nogi",
      "year": "1991",
      "journal-title": "Proc. Natl. Acad. Sci. USA."
    },
    {
      "key": "2023072902132899700_Oakesetal1993",
      "first-page": "2441",
      "article-title": "Structural alterations of the nucleolus in mutants of Saccharomyces cerevisiae defective in RNA polymerase I",
      "volume": "13",
      "author": "Oakes",
      "year": "1993",
      "journal-title": "Mol. Cell. Biol."
    },
    {
      "key": "2023072902132899700_Oakesetal1998",
      "doi-asserted-by": "crossref",
      "first-page": "23",
      "DOI": "10.1083/jcb.143.1.23",
      "article-title": "Mutational analysis of the structure and localization of the nucleolus in the yeast Saccharomyces cerevisiae",
      "volume": "143",
      "author": "Oakes",
      "year": "1998",
      "journal-title": "J. Cell Biol."
    },
    {
      "key": "2023072902132899700_Pomboetal1999",
      "doi-asserted-by": "crossref",
      "first-page": "2241",
      "DOI": "10.1093/emboj/18.8.2241",
      "article-title": "Regional specialization in human nucleivisualization of discrete sites of transcription by RNA polymerase III",
      "volume": "18",
      "author": "Pombo",
      "year": "1999",
      "journal-title": "EMBO (Eur. Mol. Biol. Organ.) J."
    },
    {
      "key": "2023072902132899700_Riggsetal1995",
      "doi-asserted-by": "crossref",
      "first-page": "6205",
      "DOI": "10.1074/jbc.270.11.6205",
      "article-title": "Characterization of the components of reconstituted Saccharomyces cerevisiae RNA polymerase I transcription complexes",
      "volume": "270",
      "author": "Riggs",
      "year": "1995",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "2023072902132899700_Saez-VasquezandPikaard1997",
      "doi-asserted-by": "crossref",
      "first-page": "11869",
      "DOI": "10.1073/pnas.94.22.11869",
      "article-title": "Extensive purification of a putative RNA polymerase I holoenzyme from plants that accurately initiates rRNA gene transcription in vitro",
      "volume": "94",
      "author": "Saez-Vasquez",
      "year": "1997",
      "journal-title": "Proc. Natl. Acad. Sci. USA."
    },
    {
      "key": "2023072902132899700_Sambrooketal1989",
      "volume-title": "Molecular CloningA Laboratory Manual",
      "author": "Sambrook",
      "year": "1989"
    },
    {
      "key": "2023072902132899700_ScheerandHock1999",
      "doi-asserted-by": "crossref",
      "first-page": "385",
      "DOI": "10.1016/S0955-0674(99)80054-4",
      "article-title": "Structure and function of the nucleolus",
      "volume": "11",
      "author": "Scheer",
      "year": "1999",
      "journal-title": "Curr. Opin. Cell Biol."
    },
    {
      "key": "2023072902132899700_Schultzetal1992",
      "doi-asserted-by": "crossref",
      "first-page": "697",
      "DOI": "10.1016/0092-8674(92)90233-3",
      "article-title": "Variants of the TATA-binding protein can distinguish subsets of RNA polymerase I, II, and III promoters",
      "volume": "69",
      "author": "Schultz",
      "year": "1992",
      "journal-title": "Cell."
    },
    {
      "key": "2023072902132899700_Schultzetal1990",
      "doi-asserted-by": "crossref",
      "first-page": "353",
      "DOI": "10.1016/S0022-2836(05)80326-2",
      "article-title": "Structural study of the yeast RNA polymerase A. Electron microscopy of lipid-bound molecules and two-dimensional crystals",
      "volume": "216",
      "author": "Schultz",
      "year": "1990",
      "journal-title": "J. Mol. Biol."
    },
    {
      "key": "2023072902132899700_Seitheretal1998",
      "doi-asserted-by": "crossref",
      "first-page": "43",
      "DOI": "10.1006/jmbi.1997.1434",
      "article-title": "Mammalian RNA polymerase I exists as a holoenzyme with associated basal transcription factors",
      "volume": "275",
      "author": "Seither",
      "year": "1998",
      "journal-title": "J. Mol. Biol."
    },
    {
      "key": "2023072902132899700_Shevchenkoetal1996a",
      "doi-asserted-by": "crossref",
      "first-page": "14440",
      "DOI": "10.1073/pnas.93.25.14440",
      "article-title": "Linking genome and proteome by mass spectrometrylarge-scale identification of yeast proteins from two dimensional gels",
      "volume": "93",
      "author": "Shevchenko",
      "year": "1996",
      "journal-title": "Proc. Natl. Acad. Sci. USA."
    },
    {
      "key": "2023072902132899700_Shevchenkoetal1996b",
      "doi-asserted-by": "crossref",
      "first-page": "850",
      "DOI": "10.1021/ac950914h",
      "article-title": "Mass spectrometric sequencing of proteins silver-stained polyacrylamide gels",
      "volume": "68",
      "author": "Shevchenko",
      "year": "1996",
      "journal-title": "Anal. Chem."
    },
    {
      "key": "2023072902132899700_Simosetal1996",
      "doi-asserted-by": "crossref",
      "first-page": "2270",
      "DOI": "10.1002/j.1460-2075.1996.tb00580.x",
      "article-title": "Nuclear pore proteins are involved in the biogenesis of functional tRNA",
      "volume": "15",
      "author": "Simos",
      "year": "1996",
      "journal-title": "EMBO (Eur. Mol. Biol. Organ.) J."
    },
    {
      "key": "2023072902132899700_SmithandSteitz1997",
      "doi-asserted-by": "crossref",
      "first-page": "669",
      "DOI": "10.1016/S0092-8674(00)80247-0",
      "article-title": "Sno storm in the nucleolusnew roles for myriad small RNPs",
      "volume": "89",
      "author": "Smith",
      "year": "1997",
      "journal-title": "Cell."
    },
    {
      "key": "2023072902132899700_Steffanetal1996",
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      "volume": "10",
      "author": "Steffan",
      "year": "1996",
      "journal-title": "Genes Dev."
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      "first-page": "3752",
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      "volume": "18",
      "author": "Steffan",
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      "journal-title": "Mol. Cell. Biol."
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      "first-page": "3464",
      "article-title": "Transcription by RNA polymerase I stimulates mitotic recombination in Saccharomyces cerevisiae",
      "volume": "9",
      "author": "Stewart",
      "year": "1989",
      "journal-title": "Mol. Cell. Biol."
    },
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      "key": "2023072902132899700_SzweykowskaKulinskaetal1994",
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      "article-title": "Intron-dependent formation of pseudouridines in the anticodon of Saccharomyces cerevisiae minor tRNA(Ile)",
      "volume": "13",
      "author": "Szweykowska Kulinska",
      "year": "1994",
      "journal-title": "EMBO (Eur. Mol. Biol. Organ.) J."
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      "article-title": "Function and synthesis of small nucleolar RNAs",
      "volume": "9",
      "author": "Tollervey",
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      "volume": "93",
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      "article-title": "RNA polymerase I from S. cerevisiae depends on an additional factor to release terminated transcripts from the template",
      "volume": "410",
      "author": "Tschochner",
      "year": "1997",
      "journal-title": "FEBS Lett."
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      "first-page": "107",
      "DOI": "10.1016/0022-2836(88)90382-8",
      "article-title": "Rapid initial cleavage of nascent pre-rRNA transcripts in yeast",
      "volume": "199",
      "author": "Veinot Drebot",
      "year": "1988",
      "journal-title": "J. Mol. Biol."
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      "first-page": "1629",
      "DOI": "10.1002/yea.320111607",
      "article-title": "Processing of pre-ribosomal RNA in Saccharomyces cerevisiae",
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      "author": "Venema",
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      "journal-title": "Yeast."
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      "article-title": "RRP5 is required for formation of both 18S and 5.8S rRNA in yeast",
      "volume": "15",
      "author": "Venema",
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      "journal-title": "EMBO (Eur. Mol. Biol. Organ.) J."
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      "article-title": "Presence of pre-rRNAs before activation of polymerase I transcription in the building process of nucleoli during early development of Xenopus laevis",
      "volume": "142",
      "author": "Verheggen",
      "year": "1998",
      "journal-title": "J. Cell Biol."
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      "key": "2023072902132899700_Vormetal1994",
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      "first-page": "3281",
      "DOI": "10.1021/ac00091a044",
      "article-title": "Improved resolution and very high sensitivity in MALDI TOF of matrix surfaces made by fast evaporation",
      "volume": "66",
      "author": "Vorm",
      "year": "1994",
      "journal-title": "Anal. Chem."
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      "key": "2023072902132899700_Wansinketal1993",
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      "first-page": "283",
      "DOI": "10.1083/jcb.122.2.283",
      "article-title": "Fluorescent labeling of nascent RNA reveals transcription by RNA polymerase II in domains scattered throughout the nucleus",
      "volume": "122",
      "author": "Wansink",
      "year": "1993",
      "journal-title": "J. Cell Biol."
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      "key": "2023072902132899700_Watkinsetal1998",
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      "first-page": "1549",
      "DOI": "10.1017/S1355838298980761",
      "article-title": "Cbf5p, a potential pseudouridine synthase, and Nhp2p, a putative RNA-binding protein, are present together with Gar1p in all H BOX/ACA-motif snoRNPs and constitute a common bipartite structure",
      "volume": "4",
      "author": "Watkins",
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      "journal-title": "RNA."
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      "DOI": "10.1006/excr.1996.0287",
      "article-title": "The transcription unit of ribosomal genes is attached to the nuclear skeleton",
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      "author": "Weipoltshammer",
      "year": "1996",
      "journal-title": "Exp. Cell Res."
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      "article-title": "RRN3 gene of Saccharomyces cerevisiae encodes an essential RNA polymerase I transcription factor which interacts with the polymerase independently of DNA template",
      "volume": "15",
      "author": "Yamamoto",
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      "article-title": "Experimental induction of prenucleolar bodies (PNBs) in interphase cellsinterphase PNBs show similar characteristics as those typically observed at telophase of mitosis in untreated cells",
      "volume": "105",
      "author": "Zatsepina",
      "year": "1997",
      "journal-title": "Chromosoma."
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