DOI: 10.1083/jcb.149.2.271. Dynamics of DNA Replication Factories in Living Cells

Dynamics of DNA Replication Factories in Living Cells

10.1083/jcb.149.2.271

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

DNA replication occurs in microscopically visible complexes at discrete sites (replication foci) in the nucleus. These foci consist of DNA associated with replication machineries, i.e., large protein complexes involved in DNA replication. To study the dynamics of these nuclear replication foci in living cells, we fused proliferating cell nuclear antigen (PCNA), a central component of the replication machinery, with the green fluorescent protein (GFP). Imaging of stable cell lines expressing low levels of GFP-PCNA showed that replication foci are heterogeneous in size and lifetime. Time-lapse studies revealed that replication foci clearly differ from nuclear speckles and coiled bodies as they neither show directional movements, nor do they seem to merge or divide. These four dimensional analyses suggested that replication factories are stably anchored in the nucleus and that changes in the pattern occur through gradual, coordinated, but asynchronous, assembly and disassembly throughout S phase.

Bibliography

Leonhardt, H., Rahn, H.-P., Weinzierl, P., Sporbert, A., Cremer, T., Zink, D., & Cardoso, M. C. (2000). Dynamics of DNA Replication Factories in Living Cells. The Journal of Cell Biology, 149(2), 271â280.

Authors 7

Heinrich Leonhardt (first)
Hans-Peter Rahn (additional)
Peter Weinzierl (additional)
Anje Sporbert (additional)
Thomas Cremer (additional)
Daniele Zink (additional)
M. Cristina Cardoso (additional)

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Dates

Type	When
Created	23 years ago (July 26, 2002, 12:45 p.m.)
Deposited	2 years ago (July 29, 2023, 2:37 a.m.)
Indexed	2 weeks, 4 days ago (Aug. 7, 2025, 5:12 p.m.)
Issued	25 years, 4 months ago (April 17, 2000)
Published	25 years, 4 months ago (April 17, 2000)
Published Online	25 years, 4 months ago (April 17, 2000)
Published Print	25 years, 4 months ago (April 17, 2000)

Funders 0

None

BibTeX

@article{Leonhardt_2000, title={Dynamics of DNA Replication Factories in Living Cells}, volume={149}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.149.2.271}, DOI={10.1083/jcb.149.2.271}, number={2}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Leonhardt, Heinrich and Rahn, Hans-Peter and Weinzierl, Peter and Sporbert, Anje and Cremer, Thomas and Zink, Daniele and Cardoso, M. Cristina}, year={2000}, month=apr, pages={271–280} }

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