DOI: 10.1083/jcb.148.5.899. Mechanism of Residence of Cytochrome B(5), a Tail-Anchored Protein, in the Endoplasmic Reticulum

Mechanism of Residence of Cytochrome B(5), a Tail-Anchored Protein, in the Endoplasmic Reticulum

10.1083/jcb.148.5.899

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

Endoplasmic reticulum (ER) proteins maintain their residency by static retention, dynamic retrieval, or a combination of the two. Tail-anchored proteins that contain a cytosolic domain associated with the lipid bilayer via a hydrophobic stretch close to the COOH terminus are sorted within the secretory pathway by largely unknown mechanisms. Here, we have investigated the mode of insertion in the bilayer and the intracellular trafficking of cytochrome b(5) (b[5]), taken as a model for ER-resident tail-anchored proteins. We first demonstrated that b(5) can acquire a transmembrane topology posttranslationally, and then used two tagged versions of b(5), N-glyc and O-glyc b(5), containing potential N- and O-glycosylation sites, respectively, at the COOH-terminal lumenal extremity, to discriminate between retention and retrieval mechanisms. Whereas the N-linked oligosaccharide provided no evidence for retrieval from a downstream compartment, a more stringent assay based on carbohydrate acquisition by O-glyc b(5) showed that b(5) gains access to enzymes catalyzing the first steps of O-glycosylation. These results suggest that b(5) slowly recycles between the ER and the cis-Golgi complex and that dynamic retrieval as well as retention are involved in sorting of tail-anchored proteins.

Authors 5

Emanuela Pedrazzini (first)
Antonello Villa (additional)
Renato Longhi (additional)
Alessandra Bulbarelli (additional)
Nica Borgese (additional)

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 12:45 p.m.)
Deposited	2 years, 1 month ago (July 28, 2023, 10:06 p.m.)
Indexed	3 days, 5 hours ago (Aug. 29, 2025, 5:53 a.m.)
Issued	25 years, 5 months ago (March 6, 2000)
Published	25 years, 5 months ago (March 6, 2000)
Published Online	25 years, 5 months ago (March 6, 2000)
Published Print	25 years, 5 months ago (March 6, 2000)

Funders 0

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BibTeX

@article{Pedrazzini_2000, title={Mechanism of Residence of Cytochrome B(5), a Tail-Anchored Protein, in the Endoplasmic Reticulum}, volume={148}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.148.5.899}, DOI={10.1083/jcb.148.5.899}, number={5}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Pedrazzini, Emanuela and Villa, Antonello and Longhi, Renato and Bulbarelli, Alessandra and Borgese, Nica}, year={2000}, month=mar, pages={899–914} }

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