DOI: 10.1083/jcb.148.3.427. Isolation of a Ribonucleoprotein Complex Involved in mRNA Localization in <i>Drosophila</i> Oocytes

Isolation of a Ribonucleoprotein Complex Involved in mRNA Localization in Drosophila Oocytes

10.1083/jcb.148.3.427

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

Localization of bicoid (bcd) mRNA to the anterior and oskar (osk) mRNA to the posterior of the Drosophila oocyte is critical for embryonic patterning. Previous genetic studies implicated exuperantia (exu) in bcd mRNA localization, but its role in this process is not understood. We have biochemically isolated Exu and show that it is part of a large RNase-sensitive complex that contains at least seven other proteins. One of these proteins was identified as the cold shock domain RNA-binding protein Ypsilon Schachtel (Yps), which we show binds directly to Exu and colocalizes with Exu in both the oocyte and nurse cells of the Drosophila egg chamber. Surprisingly, the Exu–Yps complex contains osk mRNA. This biochemical result led us to reexamine the role of Exu in the localization of osk mRNA. We discovered that exu-null mutants are defective in osk mRNA localization in both nurse cells and the oocyte. Furthermore, both Exu/Yps particles and osk mRNA follow a similar temporal pattern of localization in which they transiently accumulate at the oocyte anterior and subsequently localize to the posterior pole. We propose that Exu is a core component of a large protein complex involved in localizing mRNAs both within nurse cells and the developing oocyte.

Bibliography

Wilhelm, J. E., Mansfield, J., Hom-Booher, N., Wang, S., Turck, C. W., Hazelrigg, T., & Vale, R. D. (2000). Isolation of a Ribonucleoprotein Complex Involved in mRNA Localization in Drosophila Oocytes. The Journal of Cell Biology, 148(3), 427â440.

Authors 7

James E. Wilhelm (first)
Jennifer Mansfield (additional)
Nora Hom-Booher (additional)
Shengxian Wang (additional)
Christoph W. Turck (additional)
Tulle Hazelrigg (additional)
Ronald D. Vale (additional)

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 12:45 p.m.)
Deposited	2 years ago (July 28, 2023, 10:05 p.m.)
Indexed	11 months, 1 week ago (Sept. 14, 2024, 9:37 p.m.)
Issued	25 years, 6 months ago (Feb. 7, 2000)
Published	25 years, 6 months ago (Feb. 7, 2000)
Published Online	25 years, 6 months ago (Feb. 7, 2000)
Published Print	25 years, 6 months ago (Feb. 7, 2000)

Funders 0

None

BibTeX

@article{Wilhelm_2000, title={Isolation of a Ribonucleoprotein Complex Involved in mRNA Localization in Drosophila Oocytes}, volume={148}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.148.3.427}, DOI={10.1083/jcb.148.3.427}, number={3}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Wilhelm, James E. and Mansfield, Jennifer and Hom-Booher, Nora and Wang, Shengxian and Turck, Christoph W. and Hazelrigg, Tulle and Vale, Ronald D.}, year={2000}, month=feb, pages={427–440} }

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