DOI: 10.1083/jcb.148.2.363. Direct Involvement of Yeast Type I Myosins in Cdc42-Dependent Actin Polymerization

Direct Involvement of Yeast Type I Myosins in Cdc42-Dependent Actin Polymerization

10.1083/jcb.148.2.363

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

The generation of cortical actin filaments is necessary for processes such as cell motility and cell polarization. Several recent studies have demonstrated that Wiskott-Aldrich syndrome protein (WASP) family proteins and the actin-related protein (Arp) 2/3 complex are key factors in the nucleation of actin filaments in diverse eukaryotic organisms. To identify other factors involved in this process, we have isolated proteins that bind to Bee1p/Las17p, the yeast WASP-like protein, by affinity chromatography and mass spectroscopic analysis. The yeast type I myosins, Myo3p and Myo5p, have both been identified as Bee1p-interacting proteins. Like Bee1p, these myosins are essential for cortical actin assembly as assayed by in vitro reconstitution of actin nucleation sites in permeabilized yeast cells. Analysis using this assay further demonstrated that the motor activity of these myosins is required for the polymerization step, and that actin polymerization depends on phosphorylation of myosin motor domain by p21-activated kinases (PAKs), downstream effectors of the small guanosine triphosphatase, Cdc42p. The type I myosins also interact with the Arp2/3 complex through a sequence at the end of the tail domain homologous to the Arp2/3-activating region of WASP-like proteins. Combined deletions of the Arp2/3-interacting domains of Bee1p and the type I myosins abolish actin nucleation sites at the cortex, suggesting that these proteins function redundantly in the activation of the Arp2/3 complex.

Bibliography

Lechler, T., Shevchenko, A., Shevchenko, A., & Li, R. (2000). Direct Involvement of Yeast Type I Myosins in Cdc42-Dependent Actin Polymerization. The Journal of Cell Biology, 148(2), 363â374.

Authors 4

Terry Lechler (first)
Anna Shevchenko (additional)
Andrej Shevchenko (additional)
Rong Li (additional)

References 61 Referenced 182

10.1083/jcb.111.1.131 / J. Cell Biol. / CDC42 and CDC43, two additional genes involved in budding and the establishment of cell polarity in the yeast Saccharomyces cerevisiae by Adams (1990)
10.1038/322754a0 / Nature. / Propulsion of organelles isolated from Acanthamoeba along actin filaments by myosin-I by Adams (1986)
10.1038/340565a0 / Nature. / Binding of myosin I to membrane lipids by Adams (1989)
10.1083/jcb.141.6.1357 / J. Cell Biol. / The Src homology domain 3 (SH3) of a yeast type I myosin, Myo5p, binds to verprolin and is required for targeting to sites of actin polymerization by Anderson (1998)
10.1016/S0960-9822(02)00423-2 / Curr. Biol. / Two GTPases, Cdc42 and Rac, bind directly to a protein implicated in the immunodeficiency disorder Wiskott-Aldrich syndrome by Aspenstrom (1996)
10.1113/jphysiol.1994.sp020149 / J. Physiol. / Mechanism of force inhibition by 2,3-butanedione monoxime in rat cardiac muscleroles of [Ca2+] and cross-bridge kinetics by Backx (1994)
10.1016/0092-8674(90)90723-R / Cell. / Functions of the myosin ATP and actin binding sites are required for C. elegans thick filament assembly by Bejsovec (1990)
10.1073/pnas.94.4.1092 / Proc. Natl. Acad. Sci. USA. / p21-activated kinase has substrate specificity similar to Acanthamoeba myosin I heavy chain kinase and activates Acanthamoeba myosin I by Brzeska (1997)
10.1073/pnas.96.2.394 / Proc. Natl. Acad. Sci. USA. / Myosin I heavy chain kinasecloning of the full-length gene and acidic lipid-dependent activation by Rac and Cdc42 by Brzeska (1999)
10.1083/jcb.143.6.1535 / J. Cell Biol. / The localization of myosin VI at the Golgi complex and leading edge of fibroblasts and its phosphorylation and recruitment into membrane ruffles of A431 cells after growth factor stimulation by Buss (1998)
10.1021/bi00278a021 / Biochemistry. / Kinetic evidence for a monomer activation step in actin polymerization by Cooper (1983)
10.1083/jcb.131.1.179 / J. Cell Biol. / Myosin is involved in postmitotic cell spreading by Cramer (1995)
10.1016/S0968-0004(99)01442-5 / Trends Biochem. Sci. / p21-activated protein kinasea crucial component of morphological signaling? by Daniels (1999)
10.1016/S0092-8674(00)80960-5 / Cell. / Kin I kinesins are microtubule-destabilizing enzymes by Desai (1999)
10.1016/S0960-9822(98)00398-4 / Curr. Biol. / Actin cytoskeleton organization regulated by the PAK family of protein kinases by Eby (1998)
10.1083/jcb.146.6.1319 / J. Cell Biol. / Activation of the CDC42 effector N-WASP by the Shigella flexneri IcsA protein promotes actin nucleation by Arp2/3 complex and bacterial actin-based motility by Egile (1999)
{'key': '2023072901283645800_Evanetal1985', 'first-page': '3610', 'article-title': 'Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product', 'volume': '5', 'author': 'Evan', 'year': '1985', 'journal-title': 'Mol. Cell. Biol.'} / Mol. Cell. Biol. / Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product by Evan (1985)
10.1016/S0021-9258(17)39163-9 / J. Biol. Chem. / Experimental evidence for the contractile activities of Acanthamoeba myosins IA and IB by Fujisaki (1985)
10.1038/341328a0 / Nature. / Myosin I is located at the leading edge of locomoting Dictyostelium amoeba by Fukui (1989)
10.1083/jcb.133.6.1277 / J. Cell Biol. / Synthetic lethality screen identifies a novel yeast myosin I gene (MYO5)myosin proteins are required for polarization of the actin cytoskeleton by Goodson (1996)
10.1083/jcb.133.2.305 / J. Cell Biol. / Dictyostelium mutants lacking multiple classic myosin I isoforms reveal combinations of shared and distinct functions by Jung (1996)
10.1242/dev.103.4.675 / Development. / Behavior of microtubules and actin filaments in living Drosophila embryos by Kellogg (1988)
10.1083/jcb.138.1.95 / J. Cell Biol. / In vitro reconstitution of cortical actin assembly sites in budding yeast by Lechler (1997)
10.1083/jcb.136.3.649 / J. Cell Biol. / Bee1, a yeast protein with homology to Wiskott-Aldrich syndrome protein, is critical for the assembly of cortical actin cytoskeleton by Li (1997)
10.1083/jcb.128.4.599 / J. Cell Biol. / Regulation of cortical actin cytoskeleton assembly during polarized cell growth in budding yeast by Li (1995)
10.1083/jcb.140.5.1125 / J. Cell Biol. / Corequirement of specific phosphoinositides and small GTP-binding protein Cdc42 in inducing actin assembly in Xenopus egg extracts by Ma (1998)
10.1073/pnas.95.26.15362 / Proc. Natl. Acad. Sci. USA. / The Arp2/3 complex mediates actin polymerization induced by the small GTP-binding protein Cdc42 by Ma (1999)
10.1016/S0960-9822(98)00015-3 / Curr. Biol. / Scar1 and the related Wiskott-Aldrich syndrome protein, WASP, regulate the actin cytoskeleton through the Arp2/3 complex by Machesky (1998)
10.1073/pnas.96.7.3739 / Proc. Natl. Acad. Sci. USA. / Scar, a WASp-related protein, activates nucleation of actin filaments by the Arp2/3 complex by Machesky (1999)
10.1016/S0021-9258(19)75219-3 / J. Biol. Chem. / Acanthamoeba cofactor protein is a heavy chain kinase required for actin activation of the Mg2+-ATPase activity of Acanthamoeba myosin I by Maruta (1977)
10.1002/j.1460-2075.1996.tb00917.x / EMBO (Eur. Mol. Biol. Organ.) J. / N-WASP, a novel actin depolymerizing protein, regulates the cortical cytoskeletal rearrangement in a PIP2-dependent manner downstream of tyrosine kinases by Miki (1996)
10.1038/34208 / Nature. / Induction of filopodium formation by a WASP-related actin-depolymerizing protein N-WASP by Miki (1998)
10.1093/emboj/17.23.6932 / EMBO (Eur. Mol. Biol. Organ.) J. / WAVE, a novel WASP-family protein involved in actin reorganization induced by Rac by Miki (1998)
10.1146/annurev.cb.11.110195.003221 / Annu. Rev. Cell Dev. Biol. / Unconventional myosins by Mooseker (1995)
10.1083/jcb.134.1.117 / J. Cell Biol. / The Saccharomyces cerevisiae actin-related protein, Arp2, is involved in the actin cytoskeleton by Moreau (1996)
10.1016/S0960-9822(99)80187-0 / Curr. Biol. / Rho-family GTPases require the Arp2/3 complex to stimulate actin polymerization in Acanthamoeba extracts by Mullins (1999)
10.1016/S0960-9822(98)70396-3 / Curr. Biol. / The WASp homologue Las17p functions with the WIP homologue End5p/verprolin and is essential for endocytosis in yeast by Naqvi (1998)
10.1083/jcb.132.6.1053 / J. Cell Biol. / Biochemical kinetic characterization of the Acanthamoeba myosin-I ATPase by Ostap (1996)
10.1016/S0091-679X(08)61620-9 / Methods Cell Biol. / Fluorescence microscopy methods for yeast by Pringle (1989)
10.1073/pnas.94.26.14671 / Proc. Natl. Acad. Sci. USA. / WIP, a protein associated with Wiskott-Aldrich syndrome protein, induces actin polymerization and redistribution in lymphoid cells by Ramesh (1997)
10.1016/S0092-8674(00)80732-1 / Cell. / The interaction between N-WASP and the Arp2/3 complex links Cdc42-dependent signals to actin assembly by Rohatgi (1999)
10.1002/cm.970220303 / Cell Motil. Cytoskelet. / Extension of filopodia by motor-dependent actin assembly by Sheetz (1992)
10.1073/pnas.93.25.14440 / Proc. Natl. Acad. Sci. USA. / Linking genome and proteome by mass spectrometrylarge-scale identification of yeast proteins from two dimensional gels by Shevchenko (1996)
10.1021/ac950914h / Anal. Chem / Mass spectrometric sequencing of proteins from silver-stained polyacrylamide gels by Shevchenko (1996)
10.1016/S0092-8674(00)81050-8 / Cell. / Wiskott-Aldrich syndrome protein, a novel effector for the GTPase CDC42Hs, is implicated in actin polymerization by Symons (1996)
10.1091/mbc.4.2.233 / Mol. Biol. Cell. / The unconventional myosin encoded by the myoA gene plays a role in Dictyostelium motility by Titus (1993)
10.1016/S0021-9258(18)32850-3 / J. Biol. Chem. / The kinetics of actin nucleation and polymerization by Tobacman (1983)
10.1083/jcb.119.1.163 / J. Cell Biol. / Tissue distribution and subcellular localization of mammalian myosin I by Wagner (1992)
10.1038/385265a0 / Nature. / Actin polymerization is induced by Arp2/3 protein complex at the surface of Listeria monocytogenes by Welch (1997)
10.1126/science.281.5373.105 / Science. / Interaction of human Arp2/3 complex and the Listeria monocytogenes ActA protein in actin filament nucleation by Welch (1998)
10.1038/46835 / Nature. / Myosin VI is an actin-based motor that moves backward by Wells (1999)
10.1016/S0960-9822(06)00223-5 / Curr. Biol. / The complex containing actin-related proteins Arp2 and Arp3 is required for the motility and integrity of yeast actin patches by Winter (1997)
10.1016/S0960-9822(99)80218-8 / Curr. Biol. / Activation of the yeast Arp2/3 complex by Bee1p, a WASP family protein by Winter (1999)
10.1073/pnas.96.13.7288 / Proc. Natl. Acad. Sci. USA. / Genetic dissection of the yeast Arp2/3 complexa comparison of the in vivo and structural roles of individual subunits by Winter (1999)
10.1074/jbc.271.50.31787 / J. Biol. Chem. / Activation of myosin-I by members of the Ste20p protein kinase family by Wu (1996)
10.1074/jbc.272.49.30623 / J. Biol. Chem. / The phosphorylation site for Ste20p-like protein kinases is essential for the function of myosin-I in yeast by Wu (1997)
10.1073/pnas.94.8.3685 / Proc. Natl. Acad. Sci. USA. / The myosin-I binding protein Acan125 binds the SH3 domain and belongs to the superfamily of leucine-rich repeat proteins by Xu (1997)
10.1016/S0960-9822(99)80243-7 / Curr. Biol. / The Wiskott-Aldrich syndrome protein directs actin-based motility by stimulating actin nucleation with the Arp2/3 complex by Yarar (1999)
10.1083/jcb.138.2.363 / J. Cell Biol. / Regulation of actin polymerization in cell-free systems by GTPγS and Cdc42 by Zigmond (1997)
10.1083/jcb.142.4.1001 / J. Cell Biol. / Mechanism of Cdc42-induced actin polymerization in neutrophil extracts by Zigmond (1998)
10.1083/jcb.116.2.367 / J. Cell Biol / Myosin-I moves actin filaments on a phospholipid substrateimplications for membrane targeting by Zot (1992)

Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 12:45 p.m.)
Deposited	2 years, 1 month ago (July 28, 2023, 9:29 p.m.)
Indexed	1 month, 3 weeks ago (July 7, 2025, 2:27 a.m.)
Issued	25 years, 7 months ago (Jan. 24, 2000)
Published	25 years, 7 months ago (Jan. 24, 2000)
Published Online	25 years, 7 months ago (Jan. 24, 2000)
Published Print	25 years, 7 months ago (Jan. 24, 2000)

Funders 0

None

BibTeX

@article{Lechler_2000, title={Direct Involvement of Yeast Type I Myosins in Cdc42-Dependent Actin Polymerization}, volume={148}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.148.2.363}, DOI={10.1083/jcb.148.2.363}, number={2}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Lechler, Terry and Shevchenko, Anna and Shevchenko, Andrej and Li, Rong}, year={2000}, month=jan, pages={363–374} }

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      "article-title": "CDC42 and CDC43, two additional genes involved in budding and the establishment of cell polarity in the yeast Saccharomyces cerevisiae",
      "volume": "111",
      "author": "Adams",
      "year": "1990",
      "journal-title": "J. Cell Biol."
    },
    {
      "key": "2023072901283645800_AdamsandPollard1986",
      "doi-asserted-by": "crossref",
      "first-page": "754",
      "DOI": "10.1038/322754a0",
      "article-title": "Propulsion of organelles isolated from Acanthamoeba along actin filaments by myosin-I",
      "volume": "322",
      "author": "Adams",
      "year": "1986",
      "journal-title": "Nature."
    },
    {
      "key": "2023072901283645800_AdamsandPollard1989",
      "doi-asserted-by": "crossref",
      "first-page": "565",
      "DOI": "10.1038/340565a0",
      "article-title": "Binding of myosin I to membrane lipids",
      "volume": "340",
      "author": "Adams",
      "year": "1989",
      "journal-title": "Nature."
    },
    {
      "key": "2023072901283645800_Andersonetal1998",
      "doi-asserted-by": "crossref",
      "first-page": "1357",
      "DOI": "10.1083/jcb.141.6.1357",
      "article-title": "The Src homology domain 3 (SH3) of a yeast type I myosin, Myo5p, binds to verprolin and is required for targeting to sites of actin polymerization",
      "volume": "141",
      "author": "Anderson",
      "year": "1998",
      "journal-title": "J. Cell Biol."
    },
    {
      "key": "2023072901283645800_Aspenstrometal1996",
      "doi-asserted-by": "crossref",
      "first-page": "70",
      "DOI": "10.1016/S0960-9822(02)00423-2",
      "article-title": "Two GTPases, Cdc42 and Rac, bind directly to a protein implicated in the immunodeficiency disorder Wiskott-Aldrich syndrome",
      "volume": "6",
      "author": "Aspenstrom",
      "year": "1996",
      "journal-title": "Curr. Biol."
    },
    {
      "key": "2023072901283645800_Backxetal1994",
      "doi-asserted-by": "crossref",
      "first-page": "487",
      "DOI": "10.1113/jphysiol.1994.sp020149",
      "article-title": "Mechanism of force inhibition by 2,3-butanedione monoxime in rat cardiac muscleroles of [Ca2+] and cross-bridge kinetics",
      "volume": "476",
      "author": "Backx",
      "year": "1994",
      "journal-title": "J. Physiol."
    },
    {
      "key": "2023072901283645800_BejsovecandAnderson1990",
      "doi-asserted-by": "crossref",
      "first-page": "133",
      "DOI": "10.1016/0092-8674(90)90723-R",
      "article-title": "Functions of the myosin ATP and actin binding sites are required for C. elegans thick filament assembly",
      "volume": "60",
      "author": "Bejsovec",
      "year": "1990",
      "journal-title": "Cell."
    },
    {
      "key": "2023072901283645800_Brzeskaetal1997",
      "doi-asserted-by": "crossref",
      "first-page": "1092",
      "DOI": "10.1073/pnas.94.4.1092",
      "article-title": "p21-activated kinase has substrate specificity similar to Acanthamoeba myosin I heavy chain kinase and activates Acanthamoeba myosin I",
      "volume": "18",
      "author": "Brzeska",
      "year": "1997",
      "journal-title": "Proc. Natl. Acad. Sci. USA."
    },
    {
      "key": "2023072901283645800_Brzeskaetal1999",
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      "article-title": "Myosin I heavy chain kinasecloning of the full-length gene and acidic lipid-dependent activation by Rac and Cdc42",
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      "author": "Brzeska",
      "year": "1999",
      "journal-title": "Proc. Natl. Acad. Sci. USA."
    },
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      "volume": "143",
      "author": "Buss",
      "year": "1998",
      "journal-title": "J. Cell Biol."
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      "key": "2023072901283645800_Cooperetal1983",
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      "author": "Cooper",
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      "article-title": "Myosin is involved in postmitotic cell spreading",
      "volume": "131",
      "author": "Cramer",
      "year": "1995",
      "journal-title": "J. Cell Biol."
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      "key": "2023072901283645800_DanielsandBokoch1999",
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      "volume": "24",
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      "volume": "260",
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      "volume": "341",
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      "year": "1989",
      "journal-title": "Nature."
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      "key": "2023072901283645800_Goodsonetal1996",
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      "first-page": "1277",
      "DOI": "10.1083/jcb.133.6.1277",
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      "volume": "133",
      "author": "Goodson",
      "year": "1996",
      "journal-title": "J. Cell Biol."
    },
    {
      "key": "2023072901283645800_Jungetal1996",
      "doi-asserted-by": "crossref",
      "first-page": "305",
      "DOI": "10.1083/jcb.133.2.305",
      "article-title": "Dictyostelium mutants lacking multiple classic myosin I isoforms reveal combinations of shared and distinct functions",
      "volume": "133",
      "author": "Jung",
      "year": "1996",
      "journal-title": "J. Cell Biol."
    },
    {
      "key": "2023072901283645800_Kelloggetal1988",
      "doi-asserted-by": "crossref",
      "first-page": "675",
      "DOI": "10.1242/dev.103.4.675",
      "article-title": "Behavior of microtubules and actin filaments in living Drosophila embryos",
      "volume": "103",
      "author": "Kellogg",
      "year": "1988",
      "journal-title": "Development."
    },
    {
      "key": "2023072901283645800_LechlerandLi1997",
      "doi-asserted-by": "crossref",
      "first-page": "95",
      "DOI": "10.1083/jcb.138.1.95",
      "article-title": "In vitro reconstitution of cortical actin assembly sites in budding yeast",
      "volume": "138",
      "author": "Lechler",
      "year": "1997",
      "journal-title": "J. Cell Biol."
    },
    {
      "key": "2023072901283645800_Li1997",
      "doi-asserted-by": "crossref",
      "first-page": "649",
      "DOI": "10.1083/jcb.136.3.649",
      "article-title": "Bee1, a yeast protein with homology to Wiskott-Aldrich syndrome protein, is critical for the assembly of cortical actin cytoskeleton",
      "volume": "136",
      "author": "Li",
      "year": "1997",
      "journal-title": "J. Cell Biol."
    },
    {
      "key": "2023072901283645800_Lietal1995",
      "doi-asserted-by": "crossref",
      "first-page": "599",
      "DOI": "10.1083/jcb.128.4.599",
      "article-title": "Regulation of cortical actin cytoskeleton assembly during polarized cell growth in budding yeast",
      "volume": "128",
      "author": "Li",
      "year": "1995",
      "journal-title": "J. Cell Biol."
    },
    {
      "key": "2023072901283645800_Maetal1998",
      "doi-asserted-by": "crossref",
      "first-page": "1125",
      "DOI": "10.1083/jcb.140.5.1125",
      "article-title": "Corequirement of specific phosphoinositides and small GTP-binding protein Cdc42 in inducing actin assembly in Xenopus egg extracts",
      "volume": "140",
      "author": "Ma",
      "year": "1998",
      "journal-title": "J. Cell Biol."
    },
    {
      "key": "2023072901283645800_Maetal1999",
      "doi-asserted-by": "crossref",
      "first-page": "15362",
      "DOI": "10.1073/pnas.95.26.15362",
      "article-title": "The Arp2/3 complex mediates actin polymerization induced by the small GTP-binding protein Cdc42",
      "volume": "95",
      "author": "Ma",
      "year": "1999",
      "journal-title": "Proc. Natl. Acad. Sci. USA."
    },
    {
      "key": "2023072901283645800_MacheskyandInsall1998",
      "doi-asserted-by": "crossref",
      "first-page": "1347",
      "DOI": "10.1016/S0960-9822(98)00015-3",
      "article-title": "Scar1 and the related Wiskott-Aldrich syndrome protein, WASP, regulate the actin cytoskeleton through the Arp2/3 complex",
      "volume": "8",
      "author": "Machesky",
      "year": "1998",
      "journal-title": "Curr. Biol."
    },
    {
      "key": "2023072901283645800_Macheskyetal1999",
      "doi-asserted-by": "crossref",
      "first-page": "3739",
      "DOI": "10.1073/pnas.96.7.3739",
      "article-title": "Scar, a WASp-related protein, activates nucleation of actin filaments by the Arp2/3 complex",
      "volume": "96",
      "author": "Machesky",
      "year": "1999",
      "journal-title": "Proc. Natl. Acad. Sci. USA."
    },
    {
      "key": "2023072901283645800_MarutaandKorn1977",
      "doi-asserted-by": "crossref",
      "first-page": "8329",
      "DOI": "10.1016/S0021-9258(19)75219-3",
      "article-title": "Acanthamoeba cofactor protein is a heavy chain kinase required for actin activation of the Mg2+-ATPase activity of Acanthamoeba myosin I",
      "volume": "252",
      "author": "Maruta",
      "year": "1977",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "2023072901283645800_Mikietal1996",
      "doi-asserted-by": "crossref",
      "first-page": "5326",
      "DOI": "10.1002/j.1460-2075.1996.tb00917.x",
      "article-title": "N-WASP, a novel actin depolymerizing protein, regulates the cortical cytoskeletal rearrangement in a PIP2-dependent manner downstream of tyrosine kinases",
      "volume": "15",
      "author": "Miki",
      "year": "1996",
      "journal-title": "EMBO (Eur. Mol. Biol. Organ.) J."
    },
    {
      "key": "2023072901283645800_Mikietal1998a",
      "doi-asserted-by": "crossref",
      "first-page": "93",
      "DOI": "10.1038/34208",
      "article-title": "Induction of filopodium formation by a WASP-related actin-depolymerizing protein N-WASP",
      "volume": "391",
      "author": "Miki",
      "year": "1998",
      "journal-title": "Nature."
    },
    {
      "key": "2023072901283645800_Mikietal1998b",
      "doi-asserted-by": "crossref",
      "first-page": "6932",
      "DOI": "10.1093/emboj/17.23.6932",
      "article-title": "WAVE, a novel WASP-family protein involved in actin reorganization induced by Rac",
      "volume": "17",
      "author": "Miki",
      "year": "1998",
      "journal-title": "EMBO (Eur. Mol. Biol. Organ.) J."
    },
    {
      "key": "2023072901283645800_MoosekerandCheney1995",
      "doi-asserted-by": "crossref",
      "first-page": "633",
      "DOI": "10.1146/annurev.cb.11.110195.003221",
      "article-title": "Unconventional myosins",
      "volume": "11",
      "author": "Mooseker",
      "year": "1995",
      "journal-title": "Annu. Rev. Cell Dev. Biol."
    },
    {
      "key": "2023072901283645800_Moreauetal1996",
      "doi-asserted-by": "crossref",
      "first-page": "117",
      "DOI": "10.1083/jcb.134.1.117",
      "article-title": "The Saccharomyces cerevisiae actin-related protein, Arp2, is involved in the actin cytoskeleton",
      "volume": "134",
      "author": "Moreau",
      "year": "1996",
      "journal-title": "J. Cell Biol."
    },
    {
      "key": "2023072901283645800_MullinsandPollard1999",
      "doi-asserted-by": "crossref",
      "first-page": "405",
      "DOI": "10.1016/S0960-9822(99)80187-0",
      "article-title": "Rho-family GTPases require the Arp2/3 complex to stimulate actin polymerization in Acanthamoeba extracts",
      "volume": "9",
      "author": "Mullins",
      "year": "1999",
      "journal-title": "Curr. Biol."
    },
    {
      "key": "2023072901283645800_Naqvietal1998",
      "doi-asserted-by": "crossref",
      "first-page": "959",
      "DOI": "10.1016/S0960-9822(98)70396-3",
      "article-title": "The WASp homologue Las17p functions with the WIP homologue End5p/verprolin and is essential for endocytosis in yeast",
      "volume": "8",
      "author": "Naqvi",
      "year": "1998",
      "journal-title": "Curr. Biol."
    },
    {
      "key": "2023072901283645800_OstapandPollard1996",
      "doi-asserted-by": "crossref",
      "first-page": "1053",
      "DOI": "10.1083/jcb.132.6.1053",
      "article-title": "Biochemical kinetic characterization of the Acanthamoeba myosin-I ATPase",
      "volume": "132",
      "author": "Ostap",
      "year": "1996",
      "journal-title": "J. Cell Biol."
    },
    {
      "key": "2023072901283645800_Pringleetal1989",
      "doi-asserted-by": "crossref",
      "first-page": "357",
      "DOI": "10.1016/S0091-679X(08)61620-9",
      "article-title": "Fluorescence microscopy methods for yeast",
      "volume": "31",
      "author": "Pringle",
      "year": "1989",
      "journal-title": "Methods Cell Biol."
    },
    {
      "key": "2023072901283645800_Rameshetal1997",
      "doi-asserted-by": "crossref",
      "first-page": "14671",
      "DOI": "10.1073/pnas.94.26.14671",
      "article-title": "WIP, a protein associated with Wiskott-Aldrich syndrome protein, induces actin polymerization and redistribution in lymphoid cells",
      "volume": "94",
      "author": "Ramesh",
      "year": "1997",
      "journal-title": "Proc. Natl. Acad. Sci. USA."
    },
    {
      "key": "2023072901283645800_Rohatgietal1999",
      "doi-asserted-by": "crossref",
      "first-page": "221",
      "DOI": "10.1016/S0092-8674(00)80732-1",
      "article-title": "The interaction between N-WASP and the Arp2/3 complex links Cdc42-dependent signals to actin assembly",
      "volume": "97",
      "author": "Rohatgi",
      "year": "1999",
      "journal-title": "Cell."
    },
    {
      "key": "2023072901283645800_Sheetzetal1992",
      "doi-asserted-by": "crossref",
      "first-page": "160",
      "DOI": "10.1002/cm.970220303",
      "article-title": "Extension of filopodia by motor-dependent actin assembly",
      "volume": "22",
      "author": "Sheetz",
      "year": "1992",
      "journal-title": "Cell Motil. Cytoskelet."
    },
    {
      "key": "2023072901283645800_Shevchenkoetal1996a",
      "doi-asserted-by": "crossref",
      "first-page": "14440",
      "DOI": "10.1073/pnas.93.25.14440",
      "article-title": "Linking genome and proteome by mass spectrometrylarge-scale identification of yeast proteins from two dimensional gels",
      "volume": "93",
      "author": "Shevchenko",
      "year": "1996",
      "journal-title": "Proc. Natl. Acad. Sci. USA."
    },
    {
      "key": "2023072901283645800_Shevchenkoetal1996b",
      "doi-asserted-by": "crossref",
      "first-page": "850",
      "DOI": "10.1021/ac950914h",
      "article-title": "Mass spectrometric sequencing of proteins from silver-stained polyacrylamide gels",
      "volume": "68",
      "author": "Shevchenko",
      "year": "1996",
      "journal-title": "Anal. Chem"
    },
    {
      "key": "2023072901283645800_Symonsetal1996",
      "doi-asserted-by": "crossref",
      "first-page": "723",
      "DOI": "10.1016/S0092-8674(00)81050-8",
      "article-title": "Wiskott-Aldrich syndrome protein, a novel effector for the GTPase CDC42Hs, is implicated in actin polymerization",
      "volume": "84",
      "author": "Symons",
      "year": "1996",
      "journal-title": "Cell."
    },
    {
      "key": "2023072901283645800_Titusetal1993",
      "doi-asserted-by": "crossref",
      "first-page": "233",
      "DOI": "10.1091/mbc.4.2.233",
      "article-title": "The unconventional myosin encoded by the myoA gene plays a role in Dictyostelium motility",
      "volume": "4",
      "author": "Titus",
      "year": "1993",
      "journal-title": "Mol. Biol. Cell."
    },
    {
      "key": "2023072901283645800_TobacmanandKorn1983",
      "doi-asserted-by": "crossref",
      "first-page": "3207",
      "DOI": "10.1016/S0021-9258(18)32850-3",
      "article-title": "The kinetics of actin nucleation and polymerization",
      "volume": "258",
      "author": "Tobacman",
      "year": "1983",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "2023072901283645800_Wagneretal1992",
      "doi-asserted-by": "crossref",
      "first-page": "163",
      "DOI": "10.1083/jcb.119.1.163",
      "article-title": "Tissue distribution and subcellular localization of mammalian myosin I",
      "volume": "119",
      "author": "Wagner",
      "year": "1992",
      "journal-title": "J. Cell Biol."
    },
    {
      "key": "2023072901283645800_Welchetal1997",
      "doi-asserted-by": "crossref",
      "first-page": "265",
      "DOI": "10.1038/385265a0",
      "article-title": "Actin polymerization is induced by Arp2/3 protein complex at the surface of Listeria monocytogenes",
      "volume": "385",
      "author": "Welch",
      "year": "1997",
      "journal-title": "Nature."
    },
    {
      "key": "2023072901283645800_Welchetal1998",
      "doi-asserted-by": "crossref",
      "first-page": "105",
      "DOI": "10.1126/science.281.5373.105",
      "article-title": "Interaction of human Arp2/3 complex and the Listeria monocytogenes ActA protein in actin filament nucleation",
      "volume": "281",
      "author": "Welch",
      "year": "1998",
      "journal-title": "Science."
    },
    {
      "key": "2023072901283645800_Wellsetal1999",
      "doi-asserted-by": "crossref",
      "first-page": "505",
      "DOI": "10.1038/46835",
      "article-title": "Myosin VI is an actin-based motor that moves backward",
      "volume": "401",
      "author": "Wells",
      "year": "1999",
      "journal-title": "Nature."
    },
    {
      "key": "2023072901283645800_Winteretal1997",
      "doi-asserted-by": "crossref",
      "first-page": "519",
      "DOI": "10.1016/S0960-9822(06)00223-5",
      "article-title": "The complex containing actin-related proteins Arp2 and Arp3 is required for the motility and integrity of yeast actin patches",
      "volume": "7",
      "author": "Winter",
      "year": "1997",
      "journal-title": "Curr. Biol."
    },
    {
      "key": "2023072901283645800_Winteretal1999a",
      "doi-asserted-by": "crossref",
      "first-page": "501",
      "DOI": "10.1016/S0960-9822(99)80218-8",
      "article-title": "Activation of the yeast Arp2/3 complex by Bee1p, a WASP family protein",
      "volume": "9",
      "author": "Winter",
      "year": "1999",
      "journal-title": "Curr. Biol."
    },
    {
      "key": "2023072901283645800_Winteretal1999b",
      "doi-asserted-by": "crossref",
      "first-page": "7288",
      "DOI": "10.1073/pnas.96.13.7288",
      "article-title": "Genetic dissection of the yeast Arp2/3 complexa comparison of the in vivo and structural roles of individual subunits",
      "volume": "96",
      "author": "Winter",
      "year": "1999",
      "journal-title": "Proc. Natl. Acad. Sci. USA."
    },
    {
      "key": "2023072901283645800_Wuetal1996",
      "doi-asserted-by": "crossref",
      "first-page": "31787",
      "DOI": "10.1074/jbc.271.50.31787",
      "article-title": "Activation of myosin-I by members of the Ste20p protein kinase family",
      "volume": "271",
      "author": "Wu",
      "year": "1996",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "2023072901283645800_Wuetal1997",
      "doi-asserted-by": "crossref",
      "first-page": "30623",
      "DOI": "10.1074/jbc.272.49.30623",
      "article-title": "The phosphorylation site for Ste20p-like protein kinases is essential for the function of myosin-I in yeast",
      "volume": "272",
      "author": "Wu",
      "year": "1997",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "2023072901283645800_Xuetal1997",
      "doi-asserted-by": "crossref",
      "first-page": "3685",
      "DOI": "10.1073/pnas.94.8.3685",
      "article-title": "The myosin-I binding protein Acan125 binds the SH3 domain and belongs to the superfamily of leucine-rich repeat proteins",
      "volume": "94",
      "author": "Xu",
      "year": "1997",
      "journal-title": "Proc. Natl. Acad. Sci. USA."
    },
    {
      "key": "2023072901283645800_Yararetal1999",
      "doi-asserted-by": "crossref",
      "first-page": "555",
      "DOI": "10.1016/S0960-9822(99)80243-7",
      "article-title": "The Wiskott-Aldrich syndrome protein directs actin-based motility by stimulating actin nucleation with the Arp2/3 complex",
      "volume": "9",
      "author": "Yarar",
      "year": "1999",
      "journal-title": "Curr. Biol."
    },
    {
      "key": "2023072901283645800_Zigmondetal1997",
      "doi-asserted-by": "crossref",
      "first-page": "363",
      "DOI": "10.1083/jcb.138.2.363",
      "article-title": "Regulation of actin polymerization in cell-free systems by GTP\u03b3S and Cdc42",
      "volume": "138",
      "author": "Zigmond",
      "year": "1997",
      "journal-title": "J. Cell Biol."
    },
    {
      "key": "2023072901283645800_Zigmondetal1998",
      "doi-asserted-by": "crossref",
      "first-page": "1001",
      "DOI": "10.1083/jcb.142.4.1001",
      "article-title": "Mechanism of Cdc42-induced actin polymerization in neutrophil extracts",
      "volume": "142",
      "author": "Zigmond",
      "year": "1998",
      "journal-title": "J. Cell Biol."
    },
    {
      "key": "2023072901283645800_Zotetal1992",
      "doi-asserted-by": "crossref",
      "first-page": "367",
      "DOI": "10.1083/jcb.116.2.367",
      "article-title": "Myosin-I moves actin filaments on a phospholipid substrateimplications for membrane targeting",
      "volume": "116",
      "author": "Zot",
      "year": "1992",
      "journal-title": "J. Cell Biol"
    }
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