DOI: 10.1083/jcb.148.2.343. Homeobox B3 Promotes Capillary Morphogenesis and Angiogenesis

Homeobox B3 Promotes Capillary Morphogenesis and Angiogenesis

10.1083/jcb.148.2.343

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

Endothelial cells (EC) express several members of the Homeobox (Hox) gene family, suggesting a role for these morphoregulatory mediators during angiogenesis. We have previously established that Hox D3 is required for expression of integrin αvβ3 and urokinase plasminogen activator (uPA), which contribute to EC adhesion, invasion, and migration during angiogenesis. We now report that the paralogous gene, Hox B3, influences angiogenic behavior in a manner that is distinct from Hox D3. Antisense against Hox B3 impaired capillary morphogenesis of dermal microvascular EC cultured on basement membrane extracellular matrices. Although levels of Hox D3-dependent genes were maintained in these cells, levels of the ephrin A1 ligand were markedly attenuated. Capillary morphogenesis could be restored, however, by addition of recombinant ephrin A1/Fc fusion proteins. To test the impact of Hox B3 on angiogenesis in vivo, we constitutively expressed Hox B3 in the chick chorioallantoic membrane using avian retroviruses that resulted in an increase in vascular density and angiogenesis. Thus, while Hox D3 promotes the invasive or migratory behavior of EC, Hox B3 is required for the subsequent capillary morphogenesis of these new vascular sprouts and, together, these results support the hypothesis that paralogous Hox genes perform complementary functions within a particular tissue type.

Bibliography

Myers, C., Charboneau, A., & Boudreau, N. (2000). Homeobox B3 Promotes Capillary Morphogenesis and Angiogenesis. The Journal of Cell Biology, 148(2), 343â352.

Authors 3

Connie Myers (first)
Aubri Charboneau (additional)
Nancy Boudreau (additional)

References 37 Referenced 120

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 12:45 p.m.)
Deposited	2 years, 1 month ago (July 28, 2023, 9:37 p.m.)
Indexed	2 months ago (June 26, 2025, 2:42 p.m.)
Issued	25 years, 7 months ago (Jan. 24, 2000)
Published	25 years, 7 months ago (Jan. 24, 2000)
Published Online	25 years, 7 months ago (Jan. 24, 2000)
Published Print	25 years, 7 months ago (Jan. 24, 2000)

Funders 0

None

BibTeX

@article{Myers_2000, title={Homeobox B3 Promotes Capillary Morphogenesis and Angiogenesis}, volume={148}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.148.2.343}, DOI={10.1083/jcb.148.2.343}, number={2}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Myers, Connie and Charboneau, Aubri and Boudreau, Nancy}, year={2000}, month=jan, pages={343–352} }

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