DOI: 10.1083/jcb.148.2.283. Promyelocytic Leukemia (Pml) Nuclear Bodies Are Protein Structures That Do Not Accumulate RNA

Promyelocytic Leukemia (Pml) Nuclear Bodies Are Protein Structures That Do Not Accumulate RNA

10.1083/jcb.148.2.283

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

The promyelocytic leukemia (PML) nuclear body (also referred to as ND10, POD, and Kr body) is involved in oncogenesis and viral infection. This subnuclear domain has been reported to be rich in RNA and a site of nascent RNA synthesis, implicating its direct involvement in the regulation of gene expression. We used an analytical transmission electron microscopic method to determine the structure and composition of PML nuclear bodies and the surrounding nucleoplasm. Electron spectroscopic imaging (ESI) demonstrates that the core of the PML nuclear body is a dense, protein-based structure, 250 nm in diameter, which does not contain detectable nucleic acid. Although PML nuclear bodies contain neither chromatin nor nascent RNA, newly synthesized RNA is associated with the periphery of the PML nuclear body, and is found within the chromatin-depleted region of the nucleoplasm immediately surrounding the core of the PML nuclear body. We further show that the RNA does not accumulate in the protein core of the structure. Our results dismiss the hypothesis that the PML nuclear body is a site of transcription, but support the model in which the PML nuclear body may contribute to the formation of a favorable nuclear environment for the expression of specific genes.

Bibliography

Boisvert, F.-M., Hendzel, M. J., & Bazett-Jones, D. P. (2000). Promyelocytic Leukemia (Pml) Nuclear Bodies Are Protein Structures That Do Not Accumulate RNA. The Journal of Cell Biology, 148(2), 283â292.

Authors 3

François-Michel Boisvert (first)
Michael J. Hendzel (additional)
David P. Bazett-Jones (additional)

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 12:45 p.m.)
Deposited	2 years, 1 month ago (July 28, 2023, 9:33 p.m.)
Indexed	1 month, 2 weeks ago (July 12, 2025, 6:47 p.m.)
Issued	25 years, 7 months ago (Jan. 24, 2000)
Published	25 years, 7 months ago (Jan. 24, 2000)
Published Online	25 years, 7 months ago (Jan. 24, 2000)
Published Print	25 years, 7 months ago (Jan. 24, 2000)

Funders 0

None

BibTeX

@article{Boisvert_2000, title={Promyelocytic Leukemia (Pml) Nuclear Bodies Are Protein Structures That Do Not Accumulate RNA}, volume={148}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.148.2.283}, DOI={10.1083/jcb.148.2.283}, number={2}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Boisvert, François-Michel and Hendzel, Michael J. and Bazett-Jones, David P.}, year={2000}, month=jan, pages={283–292} }

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