DOI: 10.1083/jcb.148.1.45. Correlative Light-Electron Microscopy Reveals the Tubular-Saccular Ultrastructure of Carriers Operating between Golgi Apparatus and Plasma Membrane

Correlative Light-Electron Microscopy Reveals the Tubular-Saccular Ultrastructure of Carriers Operating between Golgi Apparatus and Plasma Membrane

10.1083/jcb.148.1.45

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

Transport intermediates (TIs) have a central role in intracellular traffic, and much effort has been directed towards defining their molecular organization. Unfortunately, major uncertainties remain regarding their true structure in living cells. To address this question, we have developed an approach based on the combination of the green fluorescent protein technology and correlative light-electron microscopy, by which it is possible to monitor an individual carrier in vivo and then take a picture of its ultrastructure at any moment of its lifecycle. We have applied this technique to define the structure of TIs operating from the Golgi apparatus to the plasma membrane, whose in vivo dynamics have been characterized recently by light microscopy. We find that these carriers are large (ranging from 0.3–1.7 μm in maximum diameter, nearly half the size of a Golgi cisterna), comprise almost exclusively tubular-saccular structures, and fuse directly with the plasma membrane, sometimes minutes after docking to the fusion site.

Bibliography

Polishchuk, R. S., Polishchuk, E. V., Marra, P., Alberti, S., Buccione, R., Luini, A., & Mironov, A. A. (2000). Correlative Light-Electron Microscopy Reveals the Tubular-Saccular Ultrastructure of Carriers Operating between Golgi Apparatus and Plasma Membrane. The Journal of Cell Biology, 148(1), 45â58.

Authors 7

Roman S. Polishchuk (first)
Elena V. Polishchuk (additional)
Pierfrancesco Marra (additional)
Saverio Alberti (additional)
Roberto Buccione (additional)
Alberto Luini (additional)
Alexander A. Mironov (additional)

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Dates

Type	When
Created	23 years ago (July 26, 2002, 12:45 p.m.)
Deposited	2 years ago (July 28, 2023, 10 p.m.)
Indexed	6 days, 9 hours ago (Aug. 19, 2025, 6:21 a.m.)
Issued	25 years, 7 months ago (Jan. 10, 2000)
Published	25 years, 7 months ago (Jan. 10, 2000)
Published Online	25 years, 7 months ago (Jan. 10, 2000)
Published Print	25 years, 7 months ago (Jan. 10, 2000)

Funders 0

None

BibTeX

@article{Polishchuk_2000, title={Correlative Light-Electron Microscopy Reveals the Tubular-Saccular Ultrastructure of Carriers Operating between Golgi Apparatus and Plasma Membrane}, volume={148}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.148.1.45}, DOI={10.1083/jcb.148.1.45}, number={1}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Polishchuk, Roman S. and Polishchuk, Elena V. and Marra, Pierfrancesco and Alberti, Saverio and Buccione, Roberto and Luini, Alberto and Mironov, Alexander A.}, year={2000}, month=jan, pages={45–58} }

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