DOI: 10.1083/jcb.147.7.1457. ER/Golgi Intermediates Acquire Golgi Enzymes by Brefeldin a

ER/Golgi Intermediates Acquire Golgi Enzymes by Brefeldin a–Sensitive Retrograde Transport in Vitro

10.1083/jcb.147.7.1457

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

Secretory proteins exit the ER in transport vesicles that fuse to form vesicular tubular clusters (VTCs) which move along microtubule tracks to the Golgi apparatus. Using the well-characterized in vitro approach to study the properties of Golgi membranes, we determined whether the Golgi enzyme NAGT I is transported to ER/Golgi intermediates. Secretory cargo was arrested at distinct steps of the secretory pathway of a glycosylation mutant cell line, and in vitro complementation of the glycosylation defect was determined. Complementation yield increased after ER exit of secretory cargo and was optimal when transport was blocked at an ER/Golgi intermediate step. The rapid drop of the complementation yield as secretory cargo progresses into the stack suggests that Golgi enzymes are preferentially targeted to ER/Golgi intermediates and not to membranes of the Golgi stack. Two mechanisms for in vitro complementation could be distinguished due to their different sensitivities to brefeldin A (BFA). Transport occurred either by direct fusion of preexisting transport intermediates with ER/Golgi intermediates, or it occurred as a BFA-sensitive and most likely COP I–mediated step. Direct fusion of ER/Golgi intermediates with cisternal membranes of the Golgi stack was not observed under these conditions.

Bibliography

Lin, C.-C., Love, H. D., Gushue, J. N., Bergeron, J. J. M., & Ostermann, J. (1999). ER/Golgi Intermediates Acquire Golgi Enzymes by Brefeldin aâSensitive Retrograde Transport in Vitro. The Journal of Cell Biology, 147(7), 1457â1472.

Authors 5

Chung-Chih Lin (first)
Harold D. Love (additional)
Jennifer N. Gushue (additional)
John J.M. Bergeron (additional)
Joachim Ostermann (additional)

References 52 Referenced 31

10.1016/S0021-9258(18)66925-X / J. Biol. Chem. / ATP-coupled transport of vesicular stomatitis virus G protein between the endoplasmic reticulum and the Golgi by Balch (1986)
10.1083/jcb.138.1.1 / J. Cell Biol. / Membrane dynamics at the endoplasmic reticulum Golgi interface by Bannykh (1997)
10.1016/S0074-7696(08)60273-9 / Int. Rev. Cytol. / The Golgi apparatusstructure and function by Beams (1968)
10.1128/mr.60.4.697-721.1996 / Microbiol. Rev / The secretory pathway of protistsspatial and functional organization and evolution by Becker (1996)
10.1074/jbc.271.43.26939 / J. Biol. Chem. / Erv25p, a component of COP II-coated vesicles, forms a complex with Emp24p that is required for efficient endoplasmic reticulum to Golgi transport by Belden (1996)
10.1016/S0092-8674(00)81723-7 / Cell. / Procollagen traverses the Golgi stack without leaving the lumen of cisternaeevidence for cisternal maturation by Bonfanti (1999)
10.1016/0092-8674(84)90458-6 / Cell. / The glycoprotein that is transported between successive compartments of the Golgi in a cell-free system resides in stacks of cisternae by Braell (1984)
10.1016/0092-8674(90)90482-T / Cell. / SNAPs, a family of NSF attachment proteins involved in intracellular membrane fusion in animals and yeast by Clary (1990)
10.1002/ar.1092400405 / Anat. Rec. / Connections between the various elements of the cis- and mid-compartments of the Golgi apparatus of early rat spermatids by Clermont (1994)
10.1083/jcb.140.1.1 / J. Cell Biol. / Retrograde transport of Golgi-localized proteins to the ER by Cole (1998)
10.1083/jcb.140.4.751 / J. Cell Biol. / gp25L/emp24/p24 protein family members of the cis-Golgi network bind COP I and II coatomer by Dominguez (1998)
10.1083/jcb.145.4.673 / J. Cell Biol. / Fusogenic domains of Golgi membranes are sequestered into specialized regions of the stack that can be released by mechanical fragmentation by Dominguez (1999)
10.1126/science.1957170 / Science. / Binding of ARF and beta-COP to Golgi membranespossible regulation by a trimeric G protein by Donaldson (1991)
10.1083/jcb.124.4.415 / J. Cell Biol. / ADP-ribosylation factor and coatomer couple fusion to vesicle budding by Elazar (1994)
10.1146/annurev.cb.01.110185.002311 / Annu. Rev. Cell Biol. / Progress in unraveling pathways of Golgi traffic by Farquhar (1985)
10.1016/S0962-8924(97)01187-2 / Trends Cell Biol. / The Golgi apparatus100 years of progress and controversy by Farquhar (1998)
10.1073/pnas.77.7.3870 / Proc. Natl. Acad. Sci. USA. / Transport of vesicular stomatitis virus glycoprotein in a cell-free extract by Fries (1980)
10.1016/S0014-5793(97)00984-8 / FEBS Lett. / A cisternal maturation mechanism can explain the asymmetry of the Golgi stack by Glick (1997)
10.1016/S0021-9258(19)41514-7 / J. Biol. Chem. / Deficient uridine diphosphate-N-acetylglucosamine:glycoprotein N-acetylglucosaminyltransferase activity in a clone of Chinese hamster ovary cells with altered surface glycoproteins by Gottlieb (1975)
10.1083/jcb.140.3.511 / J. Cell Biol. / Cell-free transport to distinct Golgi cisternae is compartment specific and ARF-independent by Happe (1998)
10.1083/jcb.134.2.295 / J. Cell Biol. / Evidence that phospholipase D mediates ADP ribosylation factor-dependent formation of Golgi-coated vesicles by Ktistakis (1996)
10.1083/jcb.144.6.1135 / J. Cell Biol. / Golgi structure in three dimensionsfunctional insights from the normal rat kidney cell by Ladinsky (1999)
10.1093/emboj/18.18.4935 / EMBO (Eur. Mol. Biol. Organ.) J. / GTP hydrolysis by arf-1 mediates sorting and concentration of Golgi resident enzymes into functional COP I vesicles by Lanoix (1999)
10.1016/0092-8674(94)90011-6 / Cell. / Coatomer is essential for retrieval of dilysine-tagged proteins to the endoplasmic reticulum by Letourneur (1994)
10.1016/S0092-8674(00)81097-1 / Cell. / SNARE-mediated retrograde traffic from the Golgi complex to the endoplasmic reticulum by Lewis (1996)
10.1007/s004180050247 / Histochem. Cell Biol. / Building a secretory apparatusrole of ARF1/COPI in Golgi biogenesis and maintenance by Lippincott-Schwartz (1998)
10.1083/jcb.140.3.541 / J. Cell Biol. / Isolation of functional Golgi-derived vesicles with a possible role in retrograde transport by Love (1998)
10.1016/S0171-9335(98)80013-7 / Eur. J. Cell Biol. / Quantitative immuno electron microscopy reveals a2,6 sialyltransferase is concentrated in the central cisternae of rat hepatocyte Golgi apparatus by Lovelock (1998)
10.1016/0092-8674(92)90027-A / Cell. / The Golgi complexin vitro veritas? by Mellman (1992)
10.1016/0092-8674(83)90154-X / Cell. / Reduced temperature prevents transfer of a glycoprotein to the cell surface but not terminal glycosylation by Matlin (1983)
10.1083/jcb.120.1.5 / J. Cell Biol. / Overlapping distribution of two glycosyltransferases in the Golgi apparatus of HeLa cells by Nilsson (1993)
10.1016/0092-8674(86)90734-8 / Cell. / A new type of coated vesicular carrier that appears not to contain clathrinits possible role in protein transport within the Golgi stack by Orci (1986)
10.1016/0092-8674(91)90273-2 / Cell. / Brefeldin A, a drug that blocks secretion, prevents the assembly of non-clathrin-coated buds on Golgi cisternae by Orci (1991)
10.1016/S0092-8674(00)80341-4 / Cell. / Bidirectional transport by distinct populations of COP I-coated vesicles by Orci (1997)
10.1073/pnas.95.5.2279 / Proc. Natl. Acad. Sci. USA. / Vesicles on strings—morphological evidence for progressive transport within the Golgi stack by Orci (1998)
10.1016/0092-8674(93)90545-2 / Cell. / Stepwise assembly of functionally active transport vesicles by Ostermann (1993)
10.1247/csf.21.413 / Cell Struct. Funct. / The dynamic organisation of the secretory pathway by Pelham (1996)
10.1083/jcb.122.6.1155 / J. Cell Biol. / Beta-COP is essential for transport of protein from the endoplasmic reticulum to the Golgi in vitro by Peter (1993)
10.1016/0092-8674(89)90872-6 / Cell. / Fatty acyl-coenzyme A is required for budding of transport vesicles from Golgi cisternae by Pfanner (1989)
10.1038/38001 / Nature. / ER-to-Golgi transport visualized in living cells by Presley (1997)
10.1002/ar.1092370402 / Anat. Rec. / Modulation of the Golgi apparatus in Saccharomyces cerevisiae sec7 mutants as seen by three-dimensional electron microscopy by Rambourg (1993)
10.1083/jcb.139.5.1119 / J. Cell Biol. / Involvement of the transmembrane protein p23 in biosynthetic protein transport by Rojo (1997)
10.1016/0092-8674(84)90508-7 / Cell. / Pre- and post-Golgi vacuoles operate in the transport of Semliki Forest virus membrane glycoproteins to the cell surface by Saraste (1984)
10.1016/S0092-8674(00)80379-7 / Cell. / Visualization of ER-to-Golgi transport in living cells reveals a sequential mode of action for COP II and COP I by Scales (1997)
10.1002/j.1460-2075.1995.tb07119.x / EMBO (Eur. Mol. Biol. Organ.) J. / The absence of Emp24p, a component of ER-derived COP II-coated vesicles, causes a defect in transport of selected proteins to the Golgi by Schimmoller (1995)
10.1083/jcb.141.4.955 / J. Cell Biol. / An ordered inheritance strategy for the Golgi apparatusvisualization of mitotic disassembly reveals a role for the mitotic spindle by Shima (1998)
10.1083/jcb.134.6.1411 / J. Cell Biol. / Sorting by COP I–coated vesicles under interphase and mitotic conditions by Sönnichsen (1996)
10.1083/jcb.140.5.1013 / J. Cell Biol. / A role for giantin in docking COP I vesicles to Golgi membranes by Sönnichsen (1998)
10.1073/pnas.92.17.8011 / Proc. Natl. Acad. Sci. USA. / An integral membrane component of coatomer-coated transport vesicles defines a family of proteins involved in budding by Stamnes (1995)
10.1083/jcb.143.6.1505 / J. Cell Biol. / Recycling of Golgi-resident glycosyltransferases through the ER reveals a novel pathway and provides an explanation for nocodazole-induced Golgi scattering by Storrie (1998)
10.1091/mbc.5.2.237 / Mol. Biol. Cell. / Cytosolic ARFs are required for vesicle formation but not for cell-free intra-Golgi transportevidence for coated vesicle-independent transport by Taylor (1994)
10.1083/jcb.122.1.39 / J. Cell Biol. / Cell type-dependent variations in the subcellular distribution of alpha-mannosidase I and II by Velasco (1993)

Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 12:45 p.m.)
Deposited	2 years, 1 month ago (July 28, 2023, 10:03 p.m.)
Indexed	1 year, 1 month ago (July 16, 2024, 3:15 p.m.)
Issued	25 years, 8 months ago (Dec. 27, 1999)
Published	25 years, 8 months ago (Dec. 27, 1999)
Published Online	25 years, 8 months ago (Dec. 27, 1999)
Published Print	25 years, 8 months ago (Dec. 27, 1999)

Funders 0

None

BibTeX

@article{Lin_1999, title={ER/Golgi Intermediates Acquire Golgi Enzymes by Brefeldin a–Sensitive Retrograde Transport in Vitro}, volume={147}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.147.7.1457}, DOI={10.1083/jcb.147.7.1457}, number={7}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Lin, Chung-Chih and Love, Harold D. and Gushue, Jennifer N. and Bergeron, John J.M. and Ostermann, Joachim}, year={1999}, month=dec, pages={1457–1472} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2024,
        7,
        16
      ]
    ],
    "date-time": "2024-07-16T19:15:07Z",
    "timestamp": 1721157307306
  },
  "reference-count": 52,
  "publisher": "Rockefeller University Press",
  "issue": "7",
  "content-domain": {
    "domain": [
      "rupress.org"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        1999,
        12,
        27
      ]
    ]
  },
  "abstract": "<jats:p>Secretory proteins exit the ER in transport vesicles that fuse to form vesicular tubular clusters (VTCs) which move along microtubule tracks to the Golgi apparatus. Using the well-characterized in vitro approach to study the properties of Golgi membranes, we determined whether the Golgi enzyme NAGT I is transported to ER/Golgi intermediates. Secretory cargo was arrested at distinct steps of the secretory pathway of a glycosylation mutant cell line, and in vitro complementation of the glycosylation defect was determined. Complementation yield increased after ER exit of secretory cargo and was optimal when transport was blocked at an ER/Golgi intermediate step. The rapid drop of the complementation yield as secretory cargo progresses into the stack suggests that Golgi enzymes are preferentially targeted to ER/Golgi intermediates and not to membranes of the Golgi stack. Two mechanisms for in vitro complementation could be distinguished due to their different sensitivities to brefeldin A (BFA). Transport occurred either by direct fusion of preexisting transport intermediates with ER/Golgi intermediates, or it occurred as a BFA-sensitive and most likely COP I\u2013mediated step. Direct fusion of ER/Golgi intermediates with cisternal membranes of the Golgi stack was not observed under these conditions.</jats:p>",
  "DOI": "10.1083/jcb.147.7.1457",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2002,
        7,
        26
      ]
    ],
    "date-time": "2002-07-26T16:45:41Z",
    "timestamp": 1027701941000
  },
  "page": "1457-1472",
  "update-policy": "http://dx.doi.org/10.1083/jcb.crossmarkpolicy",
  "source": "Crossref",
  "is-referenced-by-count": 31,
  "title": "ER/Golgi Intermediates Acquire Golgi Enzymes by Brefeldin a\u2013Sensitive Retrograde Transport in Vitro",
  "prefix": "10.1083",
  "volume": "147",
  "author": [
    {
      "given": "Chung-Chih",
      "family": "Lin",
      "sequence": "first",
      "affiliation": [
        {
          "name": "aDepartment of Biochemistry, Vanderbilt University School of Medicine, Nashville, Tennessee 37232-0146"
        }
      ]
    },
    {
      "given": "Harold D.",
      "family": "Love",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "aDepartment of Biochemistry, Vanderbilt University School of Medicine, Nashville, Tennessee 37232-0146"
        }
      ]
    },
    {
      "given": "Jennifer N.",
      "family": "Gushue",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "bDepartment of Anatomy and Cell Biology, McGill University, Montreal, Quebec, H3A 2B2 Canada"
        }
      ]
    },
    {
      "given": "John J.M.",
      "family": "Bergeron",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "bDepartment of Anatomy and Cell Biology, McGill University, Montreal, Quebec, H3A 2B2 Canada"
        }
      ]
    },
    {
      "given": "Joachim",
      "family": "Ostermann",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "aDepartment of Biochemistry, Vanderbilt University School of Medicine, Nashville, Tennessee 37232-0146"
        }
      ]
    }
  ],
  "member": "291",
  "published-online": {
    "date-parts": [
      [
        1999,
        12,
        27
      ]
    ]
  },
  "reference": [
    {
      "key": "2023072902022482900_Balchetal1986",
      "doi-asserted-by": "crossref",
      "first-page": "14681",
      "DOI": "10.1016/S0021-9258(18)66925-X",
      "article-title": "ATP-coupled transport of vesicular stomatitis virus G protein between the endoplasmic reticulum and the Golgi",
      "volume": "261",
      "author": "Balch",
      "year": "1986",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "2023072902022482900_BannykhandBalch1997",
      "doi-asserted-by": "crossref",
      "first-page": "1",
      "DOI": "10.1083/jcb.138.1.1",
      "article-title": "Membrane dynamics at the endoplasmic reticulum Golgi interface",
      "volume": "138",
      "author": "Bannykh",
      "year": "1997",
      "journal-title": "J. Cell Biol."
    },
    {
      "key": "2023072902022482900_BeamsandKessel1968",
      "doi-asserted-by": "crossref",
      "first-page": "209",
      "DOI": "10.1016/S0074-7696(08)60273-9",
      "article-title": "The Golgi apparatusstructure and function",
      "volume": "23",
      "author": "Beams",
      "year": "1968",
      "journal-title": "Int. Rev. Cytol."
    },
    {
      "key": "2023072902022482900_BeckerandMelkonian1996",
      "doi-asserted-by": "crossref",
      "first-page": "697",
      "DOI": "10.1128/mr.60.4.697-721.1996",
      "article-title": "The secretory pathway of protistsspatial and functional organization and evolution",
      "volume": "60",
      "author": "Becker",
      "year": "1996",
      "journal-title": "Microbiol. Rev"
    },
    {
      "key": "2023072902022482900_BeldenandBarlowe1996",
      "doi-asserted-by": "crossref",
      "first-page": "26939",
      "DOI": "10.1074/jbc.271.43.26939",
      "article-title": "Erv25p, a component of COP II-coated vesicles, forms a complex with Emp24p that is required for efficient endoplasmic reticulum to Golgi transport",
      "volume": "271",
      "author": "Belden",
      "year": "1996",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "2023072902022482900_Bonfantietal1999",
      "doi-asserted-by": "crossref",
      "first-page": "993",
      "DOI": "10.1016/S0092-8674(00)81723-7",
      "article-title": "Procollagen traverses the Golgi stack without leaving the lumen of cisternaeevidence for cisternal maturation",
      "volume": "95",
      "author": "Bonfanti",
      "year": "1999",
      "journal-title": "Cell."
    },
    {
      "key": "2023072902022482900_Braelletal1984",
      "doi-asserted-by": "crossref",
      "first-page": "511",
      "DOI": "10.1016/0092-8674(84)90458-6",
      "article-title": "The glycoprotein that is transported between successive compartments of the Golgi in a cell-free system resides in stacks of cisternae",
      "volume": "39",
      "author": "Braell",
      "year": "1984",
      "journal-title": "Cell."
    },
    {
      "key": "2023072902022482900_Claryetal1990",
      "doi-asserted-by": "crossref",
      "first-page": "709",
      "DOI": "10.1016/0092-8674(90)90482-T",
      "article-title": "SNAPs, a family of NSF attachment proteins involved in intracellular membrane fusion in animals and yeast",
      "volume": "61",
      "author": "Clary",
      "year": "1990",
      "journal-title": "Cell."
    },
    {
      "key": "2023072902022482900_Clermontetal1994",
      "doi-asserted-by": "crossref",
      "first-page": "469",
      "DOI": "10.1002/ar.1092400405",
      "article-title": "Connections between the various elements of the cis- and mid-compartments of the Golgi apparatus of early rat spermatids",
      "volume": "240",
      "author": "Clermont",
      "year": "1994",
      "journal-title": "Anat. Rec."
    },
    {
      "key": "2023072902022482900_Coleetal1998",
      "doi-asserted-by": "crossref",
      "first-page": "1",
      "DOI": "10.1083/jcb.140.1.1",
      "article-title": "Retrograde transport of Golgi-localized proteins to the ER",
      "volume": "140",
      "author": "Cole",
      "year": "1998",
      "journal-title": "J. Cell Biol."
    },
    {
      "key": "2023072902022482900_Dominguezetal1998",
      "doi-asserted-by": "crossref",
      "first-page": "751",
      "DOI": "10.1083/jcb.140.4.751",
      "article-title": "gp25L/emp24/p24 protein family members of the cis-Golgi network bind COP I and II coatomer",
      "volume": "140",
      "author": "Dominguez",
      "year": "1998",
      "journal-title": "J. Cell Biol."
    },
    {
      "key": "2023072902022482900_Dominguezetal1999",
      "doi-asserted-by": "crossref",
      "first-page": "673",
      "DOI": "10.1083/jcb.145.4.673",
      "article-title": "Fusogenic domains of Golgi membranes are sequestered into specialized regions of the stack that can be released by mechanical fragmentation",
      "volume": "145",
      "author": "Dominguez",
      "year": "1999",
      "journal-title": "J. Cell Biol."
    },
    {
      "key": "2023072902022482900_Donaldsonetal1991",
      "doi-asserted-by": "crossref",
      "first-page": "1197",
      "DOI": "10.1126/science.1957170",
      "article-title": "Binding of ARF and beta-COP to Golgi membranespossible regulation by a trimeric G protein",
      "volume": "254",
      "author": "Donaldson",
      "year": "1991",
      "journal-title": "Science."
    },
    {
      "key": "2023072902022482900_Elazaretal1994",
      "doi-asserted-by": "crossref",
      "first-page": "415",
      "DOI": "10.1083/jcb.124.4.415",
      "article-title": "ADP-ribosylation factor and coatomer couple fusion to vesicle budding",
      "volume": "124",
      "author": "Elazar",
      "year": "1994",
      "journal-title": "J. Cell Biol."
    },
    {
      "key": "2023072902022482900_Farquhar1985",
      "doi-asserted-by": "crossref",
      "first-page": "447",
      "DOI": "10.1146/annurev.cb.01.110185.002311",
      "article-title": "Progress in unraveling pathways of Golgi traffic",
      "volume": "1",
      "author": "Farquhar",
      "year": "1985",
      "journal-title": "Annu. Rev. Cell Biol."
    },
    {
      "key": "2023072902022482900_FarquharandPalade1998",
      "doi-asserted-by": "crossref",
      "first-page": "2",
      "DOI": "10.1016/S0962-8924(97)01187-2",
      "article-title": "The Golgi apparatus100 years of progress and controversy",
      "volume": "8",
      "author": "Farquhar",
      "year": "1998",
      "journal-title": "Trends Cell Biol."
    },
    {
      "key": "2023072902022482900_FriesandRothman1980",
      "doi-asserted-by": "crossref",
      "first-page": "3870",
      "DOI": "10.1073/pnas.77.7.3870",
      "article-title": "Transport of vesicular stomatitis virus glycoprotein in a cell-free extract",
      "volume": "77",
      "author": "Fries",
      "year": "1980",
      "journal-title": "Proc. Natl. Acad. Sci. USA."
    },
    {
      "key": "2023072902022482900_Glicketal1997",
      "doi-asserted-by": "crossref",
      "first-page": "177",
      "DOI": "10.1016/S0014-5793(97)00984-8",
      "article-title": "A cisternal maturation mechanism can explain the asymmetry of the Golgi stack",
      "volume": "414",
      "author": "Glick",
      "year": "1997",
      "journal-title": "FEBS Lett."
    },
    {
      "key": "2023072902022482900_Gottliebetal1975",
      "doi-asserted-by": "crossref",
      "first-page": "3303",
      "DOI": "10.1016/S0021-9258(19)41514-7",
      "article-title": "Deficient uridine diphosphate-N-acetylglucosamine:glycoprotein N-acetylglucosaminyltransferase activity in a clone of Chinese hamster ovary cells with altered surface glycoproteins",
      "volume": "250",
      "author": "Gottlieb",
      "year": "1975",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "2023072902022482900_HappeandWeidman1998",
      "doi-asserted-by": "crossref",
      "first-page": "511",
      "DOI": "10.1083/jcb.140.3.511",
      "article-title": "Cell-free transport to distinct Golgi cisternae is compartment specific and ARF-independent",
      "volume": "140",
      "author": "Happe",
      "year": "1998",
      "journal-title": "J. Cell Biol."
    },
    {
      "key": "2023072902022482900_Ktistakisetal1996",
      "doi-asserted-by": "crossref",
      "first-page": "295",
      "DOI": "10.1083/jcb.134.2.295",
      "article-title": "Evidence that phospholipase D mediates ADP ribosylation factor-dependent formation of Golgi-coated vesicles",
      "volume": "134",
      "author": "Ktistakis",
      "year": "1996",
      "journal-title": "J. Cell Biol."
    },
    {
      "key": "2023072902022482900_Ladinskyetal1999",
      "doi-asserted-by": "crossref",
      "first-page": "1135",
      "DOI": "10.1083/jcb.144.6.1135",
      "article-title": "Golgi structure in three dimensionsfunctional insights from the normal rat kidney cell",
      "volume": "144",
      "author": "Ladinsky",
      "year": "1999",
      "journal-title": "J. Cell Biol."
    },
    {
      "key": "2023072902022482900_Lanoixetal1999",
      "doi-asserted-by": "crossref",
      "first-page": "4935",
      "DOI": "10.1093/emboj/18.18.4935",
      "article-title": "GTP hydrolysis by arf-1 mediates sorting and concentration of Golgi resident enzymes into functional COP I vesicles",
      "volume": "18",
      "author": "Lanoix",
      "year": "1999",
      "journal-title": "EMBO (Eur. Mol. Biol. Organ.) J."
    },
    {
      "key": "2023072902022482900_Letourneuretal1994",
      "doi-asserted-by": "crossref",
      "first-page": "1199",
      "DOI": "10.1016/0092-8674(94)90011-6",
      "article-title": "Coatomer is essential for retrieval of dilysine-tagged proteins to the endoplasmic reticulum",
      "volume": "79",
      "author": "Letourneur",
      "year": "1994",
      "journal-title": "Cell."
    },
    {
      "key": "2023072902022482900_LewisandPelham1996",
      "doi-asserted-by": "crossref",
      "first-page": "205",
      "DOI": "10.1016/S0092-8674(00)81097-1",
      "article-title": "SNARE-mediated retrograde traffic from the Golgi complex to the endoplasmic reticulum",
      "volume": "85",
      "author": "Lewis",
      "year": "1996",
      "journal-title": "Cell."
    },
    {
      "key": "2023072902022482900_Lippincott-Schwartzetal1998",
      "doi-asserted-by": "crossref",
      "first-page": "449",
      "DOI": "10.1007/s004180050247",
      "article-title": "Building a secretory apparatusrole of ARF1/COPI in Golgi biogenesis and maintenance",
      "volume": "109",
      "author": "Lippincott-Schwartz",
      "year": "1998",
      "journal-title": "Histochem. Cell Biol."
    },
    {
      "key": "2023072902022482900_Loveetal1998",
      "doi-asserted-by": "crossref",
      "first-page": "541",
      "DOI": "10.1083/jcb.140.3.541",
      "article-title": "Isolation of functional Golgi-derived vesicles with a possible role in retrograde transport",
      "volume": "140",
      "author": "Love",
      "year": "1998",
      "journal-title": "J. Cell Biol."
    },
    {
      "key": "2023072902022482900_LovelockandLucocq1998",
      "doi-asserted-by": "crossref",
      "first-page": "18",
      "DOI": "10.1016/S0171-9335(98)80013-7",
      "article-title": "Quantitative immuno electron microscopy reveals a2,6 sialyltransferase is concentrated in the central cisternae of rat hepatocyte Golgi apparatus",
      "volume": "76",
      "author": "Lovelock",
      "year": "1998",
      "journal-title": "Eur. J. Cell Biol."
    },
    {
      "key": "2023072902022482900_MellmanandSimons1992",
      "doi-asserted-by": "crossref",
      "first-page": "829",
      "DOI": "10.1016/0092-8674(92)90027-A",
      "article-title": "The Golgi complexin vitro veritas?",
      "volume": "68",
      "author": "Mellman",
      "year": "1992",
      "journal-title": "Cell."
    },
    {
      "key": "2023072902022482900_MatlinandSimons1983",
      "doi-asserted-by": "crossref",
      "first-page": "233",
      "DOI": "10.1016/0092-8674(83)90154-X",
      "article-title": "Reduced temperature prevents transfer of a glycoprotein to the cell surface but not terminal glycosylation",
      "volume": "34",
      "author": "Matlin",
      "year": "1983",
      "journal-title": "Cell."
    },
    {
      "key": "2023072902022482900_Nilssonetal1993",
      "doi-asserted-by": "crossref",
      "first-page": "5",
      "DOI": "10.1083/jcb.120.1.5",
      "article-title": "Overlapping distribution of two glycosyltransferases in the Golgi apparatus of HeLa cells",
      "volume": "120",
      "author": "Nilsson",
      "year": "1993",
      "journal-title": "J. Cell Biol."
    },
    {
      "key": "2023072902022482900_Orcietal1986",
      "doi-asserted-by": "crossref",
      "first-page": "171",
      "DOI": "10.1016/0092-8674(86)90734-8",
      "article-title": "A new type of coated vesicular carrier that appears not to contain clathrinits possible role in protein transport within the Golgi stack",
      "volume": "46",
      "author": "Orci",
      "year": "1986",
      "journal-title": "Cell."
    },
    {
      "key": "2023072902022482900_Orcietal1991",
      "doi-asserted-by": "crossref",
      "first-page": "1183",
      "DOI": "10.1016/0092-8674(91)90273-2",
      "article-title": "Brefeldin A, a drug that blocks secretion, prevents the assembly of non-clathrin-coated buds on Golgi cisternae",
      "volume": "64",
      "author": "Orci",
      "year": "1991",
      "journal-title": "Cell."
    },
    {
      "key": "2023072902022482900_Orcietal1997",
      "doi-asserted-by": "crossref",
      "first-page": "335",
      "DOI": "10.1016/S0092-8674(00)80341-4",
      "article-title": "Bidirectional transport by distinct populations of COP I-coated vesicles",
      "volume": "90",
      "author": "Orci",
      "year": "1997",
      "journal-title": "Cell."
    },
    {
      "key": "2023072902022482900_Orcietal1998",
      "doi-asserted-by": "crossref",
      "first-page": "2279",
      "DOI": "10.1073/pnas.95.5.2279",
      "article-title": "Vesicles on strings\u2014morphological evidence for progressive transport within the Golgi stack",
      "volume": "95",
      "author": "Orci",
      "year": "1998",
      "journal-title": "Proc. Natl. Acad. Sci. USA."
    },
    {
      "key": "2023072902022482900_Ostermannetal1993",
      "doi-asserted-by": "crossref",
      "first-page": "1015",
      "DOI": "10.1016/0092-8674(93)90545-2",
      "article-title": "Stepwise assembly of functionally active transport vesicles",
      "volume": "75",
      "author": "Ostermann",
      "year": "1993",
      "journal-title": "Cell."
    },
    {
      "key": "2023072902022482900_Pelham1996",
      "doi-asserted-by": "crossref",
      "first-page": "413",
      "DOI": "10.1247/csf.21.413",
      "article-title": "The dynamic organisation of the secretory pathway",
      "volume": "21",
      "author": "Pelham",
      "year": "1996",
      "journal-title": "Cell Struct. Funct."
    },
    {
      "key": "2023072902022482900_Peteretal1993",
      "doi-asserted-by": "crossref",
      "first-page": "1155",
      "DOI": "10.1083/jcb.122.6.1155",
      "article-title": "Beta-COP is essential for transport of protein from the endoplasmic reticulum to the Golgi in vitro",
      "volume": "122",
      "author": "Peter",
      "year": "1993",
      "journal-title": "J. Cell Biol."
    },
    {
      "key": "2023072902022482900_Pfanneretal1989",
      "doi-asserted-by": "crossref",
      "first-page": "95",
      "DOI": "10.1016/0092-8674(89)90872-6",
      "article-title": "Fatty acyl-coenzyme A is required for budding of transport vesicles from Golgi cisternae",
      "volume": "59",
      "author": "Pfanner",
      "year": "1989",
      "journal-title": "Cell."
    },
    {
      "key": "2023072902022482900_Presleyetal1997",
      "doi-asserted-by": "crossref",
      "first-page": "81",
      "DOI": "10.1038/38001",
      "article-title": "ER-to-Golgi transport visualized in living cells",
      "volume": "389",
      "author": "Presley",
      "year": "1997",
      "journal-title": "Nature."
    },
    {
      "key": "2023072902022482900_Rambourgetal1993",
      "doi-asserted-by": "crossref",
      "first-page": "441",
      "DOI": "10.1002/ar.1092370402",
      "article-title": "Modulation of the Golgi apparatus in Saccharomyces cerevisiae sec7 mutants as seen by three-dimensional electron microscopy",
      "volume": "237",
      "author": "Rambourg",
      "year": "1993",
      "journal-title": "Anat. Rec."
    },
    {
      "key": "2023072902022482900_Rojoetal1997",
      "doi-asserted-by": "crossref",
      "first-page": "1119",
      "DOI": "10.1083/jcb.139.5.1119",
      "article-title": "Involvement of the transmembrane protein p23 in biosynthetic protein transport",
      "volume": "139",
      "author": "Rojo",
      "year": "1997",
      "journal-title": "J. Cell Biol."
    },
    {
      "key": "2023072902022482900_SarasteandKuismanen1984",
      "doi-asserted-by": "crossref",
      "first-page": "535",
      "DOI": "10.1016/0092-8674(84)90508-7",
      "article-title": "Pre- and post-Golgi vacuoles operate in the transport of Semliki Forest virus membrane glycoproteins to the cell surface",
      "volume": "38",
      "author": "Saraste",
      "year": "1984",
      "journal-title": "Cell."
    },
    {
      "key": "2023072902022482900_Scalesetal1997",
      "doi-asserted-by": "crossref",
      "first-page": "1137",
      "DOI": "10.1016/S0092-8674(00)80379-7",
      "article-title": "Visualization of ER-to-Golgi transport in living cells reveals a sequential mode of action for COP II and COP I",
      "volume": "90",
      "author": "Scales",
      "year": "1997",
      "journal-title": "Cell."
    },
    {
      "key": "2023072902022482900_Schimmolleretal1995",
      "doi-asserted-by": "crossref",
      "first-page": "1329",
      "DOI": "10.1002/j.1460-2075.1995.tb07119.x",
      "article-title": "The absence of Emp24p, a component of ER-derived COP II-coated vesicles, causes a defect in transport of selected proteins to the Golgi",
      "volume": "14",
      "author": "Schimmoller",
      "year": "1995",
      "journal-title": "EMBO (Eur. Mol. Biol. Organ.) J."
    },
    {
      "key": "2023072902022482900_Shimaetal1998",
      "doi-asserted-by": "crossref",
      "first-page": "955",
      "DOI": "10.1083/jcb.141.4.955",
      "article-title": "An ordered inheritance strategy for the Golgi apparatusvisualization of mitotic disassembly reveals a role for the mitotic spindle",
      "volume": "141",
      "author": "Shima",
      "year": "1998",
      "journal-title": "J. Cell Biol."
    },
    {
      "key": "2023072902022482900_N0x3689680N0x3d54ed0",
      "doi-asserted-by": "crossref",
      "first-page": "1411",
      "DOI": "10.1083/jcb.134.6.1411",
      "article-title": "Sorting by COP I\u2013coated vesicles under interphase and mitotic conditions",
      "volume": "134",
      "author": "S\u00f6nnichsen",
      "year": "1996",
      "journal-title": "J. Cell Biol."
    },
    {
      "key": "2023072902022482900_N0x3689680N0x3d54f30",
      "doi-asserted-by": "crossref",
      "first-page": "1013",
      "DOI": "10.1083/jcb.140.5.1013",
      "article-title": "A role for giantin in docking COP I vesicles to Golgi membranes",
      "volume": "140",
      "author": "S\u00f6nnichsen",
      "year": "1998",
      "journal-title": "J. Cell Biol."
    },
    {
      "key": "2023072902022482900_Stamnesetal1995",
      "doi-asserted-by": "crossref",
      "first-page": "8011",
      "DOI": "10.1073/pnas.92.17.8011",
      "article-title": "An integral membrane component of coatomer-coated transport vesicles defines a family of proteins involved in budding",
      "volume": "92",
      "author": "Stamnes",
      "year": "1995",
      "journal-title": "Proc. Natl. Acad. Sci. USA."
    },
    {
      "key": "2023072902022482900_Storrieetal1998",
      "doi-asserted-by": "crossref",
      "first-page": "1505",
      "DOI": "10.1083/jcb.143.6.1505",
      "article-title": "Recycling of Golgi-resident glycosyltransferases through the ER reveals a novel pathway and provides an explanation for nocodazole-induced Golgi scattering",
      "volume": "143",
      "author": "Storrie",
      "year": "1998",
      "journal-title": "J. Cell Biol."
    },
    {
      "key": "2023072902022482900_Tayloretal1994",
      "doi-asserted-by": "crossref",
      "first-page": "237",
      "DOI": "10.1091/mbc.5.2.237",
      "article-title": "Cytosolic ARFs are required for vesicle formation but not for cell-free intra-Golgi transportevidence for coated vesicle-independent transport",
      "volume": "5",
      "author": "Taylor",
      "year": "1994",
      "journal-title": "Mol. Biol. Cell."
    },
    {
      "key": "2023072902022482900_Velascoetal1993",
      "doi-asserted-by": "crossref",
      "first-page": "39",
      "DOI": "10.1083/jcb.122.1.39",
      "article-title": "Cell type-dependent variations in the subcellular distribution of alpha-mannosidase I and II",
      "volume": "122",
      "author": "Velasco",
      "year": "1993",
      "journal-title": "J. Cell Biol."
    }
  ],
  "container-title": "The Journal of Cell Biology",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://rupress.org/jcb/article-pdf/147/7/1457/1857162/9907098.pdf",
      "content-type": "application/pdf",
      "content-version": "vor",
      "intended-application": "syndication"
    },
    {
      "URL": "https://rupress.org/jcb/article-pdf/147/7/1457/1857162/9907098.pdf",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2023,
        7,
        29
      ]
    ],
    "date-time": "2023-07-29T02:03:18Z",
    "timestamp": 1690596198000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://rupress.org/jcb/article/147/7/1457/20421/ER-Golgi-Intermediates-Acquire-Golgi-Enzymes-by"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1999,
        12,
        27
      ]
    ]
  },
  "references-count": 52,
  "journal-issue": {
    "issue": "7",
    "published-print": {
      "date-parts": [
        [
          1999,
          12,
          27
        ]
      ]
    }
  },
  "URL": "http://dx.doi.org/10.1083/jcb.147.7.1457",
  "relation": {},
  "ISSN": [
    "0021-9525",
    "1540-8140"
  ],
  "subject": [],
  "published": {
    "date-parts": [
      [
        1999,
        12,
        27
      ]
    ]
  }
}