DOI: 10.1083/jcb.147.5.959. The Tom Core Complex

The Tom Core Complex

10.1083/jcb.147.5.959

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

Translocation of nuclear-encoded preproteins across the outer membrane of mitochondria is mediated by the multicomponent transmembrane TOM complex. We have isolated the TOM core complex of Neurospora crassa by removing the receptors Tom70 and Tom20 from the isolated TOM holo complex by treatment with the detergent dodecyl maltoside. It consists of Tom40, Tom22, and the small Tom components, Tom6 and Tom7. This core complex was also purified directly from mitochondria after solubilization with dodecyl maltoside. The TOM core complex has the characteristics of the general insertion pore; it contains high-conductance channels and binds preprotein in a targeting sequence-dependent manner. It forms a double ring structure that, in contrast to the holo complex, lacks the third density seen in the latter particles. Three-dimensional reconstruction by electron tomography exhibits two open pores traversing the complex with a diameter of ∼2.1 nm and a height of ∼7 nm. Tom40 is the key structural element of the TOM core complex.

Bibliography

Ahting, U., Thun, C., Hegerl, R., Typke, D., Nargang, F. E., Neupert, W., & Nussberger, S. (1999). The Tom Core Complex. The Journal of Cell Biology, 147(5), 959â968.

Authors 7

Uwe Ahting (first)
Clemens Thun (additional)
Reiner Hegerl (additional)
Dieter Typke (additional)
Frank E. Nargang (additional)
Walter Neupert (additional)
Stephan Nussberger (additional)

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Dates

Type	When
Created	23 years ago (July 26, 2002, 12:45 p.m.)
Deposited	2 years ago (July 28, 2023, 9:04 p.m.)
Indexed	2 weeks, 1 day ago (Aug. 7, 2025, 4:53 p.m.)
Issued	25 years, 8 months ago (Nov. 29, 1999)
Published	25 years, 8 months ago (Nov. 29, 1999)
Published Online	25 years, 8 months ago (Nov. 29, 1999)
Published Print	25 years, 8 months ago (Nov. 29, 1999)

Funders 0

None

BibTeX

@article{Ahting_1999, title={The Tom Core Complex}, volume={147}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.147.5.959}, DOI={10.1083/jcb.147.5.959}, number={5}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Ahting, Uwe and Thun, Clemens and Hegerl, Reiner and Typke, Dieter and Nargang, Frank E. and Neupert, Walter and Nussberger, Stephan}, year={1999}, month=nov, pages={959–968} }

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