DOI: 10.1083/jcb.147.4.809. The Pro-Apoptotic Proteins, Bid and Bax, Cause a Limited Permeabilization of the Mitochondrial Outer Membrane That Is Enhanced by Cytosol

The Pro-Apoptotic Proteins, Bid and Bax, Cause a Limited Permeabilization of the Mitochondrial Outer Membrane That Is Enhanced by Cytosol

10.1083/jcb.147.4.809

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

During apoptosis, an important pathway leading to caspase activation involves the release of cytochrome c from the intermembrane space of mitochondria. Using a cell-free system based on Xenopus egg extracts, we examined changes in the outer mitochondrial membrane accompanying cytochrome c efflux. The pro-apoptotic proteins, Bid and Bax, as well as factors present in Xenopus egg cytosol, each induced cytochrome c release when incubated with isolated mitochondria. These factors caused a permeabilization of the outer membrane that allowed the corelease of multiple intermembrane space proteins: cytochrome c, adenylate kinase and sulfite oxidase. The efflux process is thus nonspecific. None of the cytochrome c-releasing factors caused detectable mitochondrial swelling, arguing that matrix swelling is not required for outer membrane permeability in this system. Bid and Bax caused complete release of cytochrome c but only a limited permeabilization of the outer membrane, as measured by the accessibility of inner membrane-associated respiratory complexes III and IV to exogenously added cytochrome c. However, outer membrane permeability was strikingly increased by a macromolecular cytosolic factor, termed PEF (permeability enhancing factor). We hypothesize that PEF activity could help determine whether cells can recover from mitochondrial cytochrome c release.

Bibliography

Kluck, R. M., Esposti, M. D., Perkins, G., Renken, C., Kuwana, T., Bossy-Wetzel, E., Goldberg, M., Allen, T., Barber, M. J., Green, D. R., & Newmeyer, D. D. (1999). The Pro-Apoptotic Proteins, Bid and Bax, Cause a Limited Permeabilization of the Mitochondrial Outer Membrane That Is Enhanced by Cytosol. The Journal of Cell Biology, 147(4), 809â822.

Authors 11

Ruth M. Kluck (first)
Mauro Degli Esposti (additional)
Guy Perkins (additional)
Christian Renken (additional)
Tomomi Kuwana (additional)
Ella Bossy-Wetzel (additional)
Martin Goldberg (additional)
Terry Allen (additional)
Michael J. Barber (additional)
Douglas R. Green (additional)
Donald D. Newmeyer (additional)

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 12:45 p.m.)
Deposited	2 years ago (July 28, 2023, 8:58 p.m.)
Indexed	1 week ago (Aug. 21, 2025, 1:15 p.m.)
Issued	25 years, 9 months ago (Nov. 15, 1999)
Published	25 years, 9 months ago (Nov. 15, 1999)
Published Online	25 years, 9 months ago (Nov. 15, 1999)
Published Print	25 years, 9 months ago (Nov. 15, 1999)

Funders 0

None

BibTeX

@article{Kluck_1999, title={The Pro-Apoptotic Proteins, Bid and Bax, Cause a Limited Permeabilization of the Mitochondrial Outer Membrane That Is Enhanced by Cytosol}, volume={147}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.147.4.809}, DOI={10.1083/jcb.147.4.809}, number={4}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Kluck, Ruth M. and Esposti, Mauro Degli and Perkins, Guy and Renken, Christian and Kuwana, Tomomi and Bossy-Wetzel, Ella and Goldberg, Martin and Allen, Terry and Barber, Michael J. and Green, Douglas R. and Newmeyer, Donald D.}, year={1999}, month=nov, pages={809–822} }

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