DOI: 10.1083/jcb.147.4.791. The Cooh-Terminal Domain of Myo2p, a Yeast Myosin V, Has a Direct Role in Secretory Vesicle Targeting

The Cooh-Terminal Domain of Myo2p, a Yeast Myosin V, Has a Direct Role in Secretory Vesicle Targeting

10.1083/jcb.147.4.791

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

MYO2 encodes a type V myosin heavy chain needed for the targeting of vacuoles and secretory vesicles to the growing bud of yeast. Here we describe new myo2 alleles containing conditional lethal mutations in the COOH-terminal tail domain. Within 5 min of shifting to the restrictive temperature, the polarized distribution of secretory vesicles is abolished without affecting the distribution of actin or the mutant Myo2p, showing that the tail has a direct role in vesicle targeting. We also show that the actin cable–dependent translocation of Myo2p to growth sites does not require secretory vesicle cargo. Although a fusion protein containing the Myo2p tail also concentrates at growth sites, this accumulation depends on the polarized delivery of secretory vesicles, implying that the Myo2p tail binds to secretory vesicles. Most of the new mutations alter a region of the Myo2p tail conserved with vertebrate myosin Vs but divergent from Myo4p, the myosin V involved in mRNA transport, and genetic data suggest that the tail interacts with Smy1p, a kinesin homologue, and Sec4p, a vesicle-associated Rab protein. The data support a model in which the Myo2p tail tethers secretory vesicles, and the motor transports them down polarized actin cables to the site of exocytosis.

Bibliography

Schott, D., Ho, J., Pruyne, D., & Bretscher, A. (1999). The Cooh-Terminal Domain of Myo2p, a Yeast Myosin V, Has a Direct Role in Secretory Vesicle Targeting. The Journal of Cell Biology, 147(4), 791â808.

Authors 4

Daniel Schott (first)
Jackson Ho (additional)
David Pruyne (additional)
Anthony Bretscher (additional)

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 12:45 p.m.)
Deposited	2 years ago (July 28, 2023, 8:55 p.m.)
Indexed	1 month, 3 weeks ago (July 2, 2025, 1:51 p.m.)
Issued	25 years, 9 months ago (Nov. 15, 1999)
Published	25 years, 9 months ago (Nov. 15, 1999)
Published Online	25 years, 9 months ago (Nov. 15, 1999)
Published Print	25 years, 9 months ago (Nov. 15, 1999)

Funders 0

None

BibTeX

@article{Schott_1999, title={The Cooh-Terminal Domain of Myo2p, a Yeast Myosin V, Has a Direct Role in Secretory Vesicle Targeting}, volume={147}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.147.4.791}, DOI={10.1083/jcb.147.4.791}, number={4}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Schott, Daniel and Ho, Jackson and Pruyne, David and Bretscher, Anthony}, year={1999}, month=nov, pages={791–808} }

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