DOI: 10.1083/jcb.147.1.13. Spatial Relationship between Transcription Sites and Chromosome Territories

Spatial Relationship between Transcription Sites and Chromosome Territories

10.1083/jcb.147.1.13

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

We have investigated the spatial relationship between transcription sites and chromosome territories in the interphase nucleus of human female fibroblasts. Immunolabeling of nascent RNA was combined with visualization of chromosome territories by fluorescent in situ hybridization (FISH). Transcription sites were found scattered throughout the territory of one of the two X chromosomes, most likely the active X chromosome, and that of both territories of chromosome 19. The other X chromosome territory, probably the inactive X chromosome, was devoid of transcription sites. A distinct substructure was observed in interphase chromosome territories. Intensely labeled subchromosomal domains are surrounded by less strongly labeled areas. The intensely labeled domains had a diameter in the range of 300–450 nm and were sometimes interconnected, forming thread-like structures. Similar large scale chromatin structures were observed in HeLa cells expressing green fluorescent protein (GFP)-tagged histone H2B. Strikingly, nascent RNA was almost exclusively found in the interchromatin areas in chromosome territories and in between strongly GFP-labeled chromatin domains. These observations support a model in which transcriptionally active chromatin in chromosome territories is markedly compartmentalized. Active loci are located predominantly at or near the surface of compact chromatin domains, depositing newly synthesized RNA directly into the interchromatin space.

Bibliography

Verschure, P. J., van der Kraan, I., Manders, E. M. M., & van Driel, R. (1999). Spatial Relationship between Transcription Sites and Chromosome Territories. The Journal of Cell Biology, 147(1), 13â24.

Authors 4

Pernette J. Verschure (first)
Ineke van der Kraan (additional)
Erik M.M. Manders (additional)
Roel van Driel (additional)

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 12:45 p.m.)
Deposited	1 year, 7 months ago (Jan. 4, 2024, 12:12 p.m.)
Indexed	1 week ago (Aug. 21, 2025, 1:49 p.m.)
Issued	25 years, 10 months ago (Oct. 4, 1999)
Published	25 years, 10 months ago (Oct. 4, 1999)
Published Online	25 years, 10 months ago (Oct. 4, 1999)
Published Print	25 years, 10 months ago (Oct. 4, 1999)

Funders 0

None

BibTeX

@article{Verschure_1999, title={Spatial Relationship between Transcription Sites and Chromosome Territories}, volume={147}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.147.1.13}, DOI={10.1083/jcb.147.1.13}, number={1}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Verschure, Pernette J. and van der Kraan, Ineke and Manders, Erik M.M. and van Driel, Roel}, year={1999}, month=oct, pages={13–24} }

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