DOI: 10.1083/jcb.146.2.313. N-Glycans Mediate the Apical Sorting of a Gpi-Anchored, Raft-Associated Protein in Madin-Darby Canine Kidney Cells

N-Glycans Mediate the Apical Sorting of a Gpi-Anchored, Raft-Associated Protein in Madin-Darby Canine Kidney Cells

10.1083/jcb.146.2.313

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

Glycosyl-phosphatidylinositol (GPI)- anchored proteins are preferentially transported to the apical cell surface of polarized Madin-Darby canine kidney (MDCK) cells. It has been assumed that the GPI anchor itself acts as an apical determinant by its interaction with sphingolipid-cholesterol rafts. We modified the rat growth hormone (rGH), an unglycosylated, unpolarized secreted protein, into a GPI-anchored protein and analyzed its surface delivery in polarized MDCK cells. The addition of a GPI anchor to rGH did not lead to an increase in apical delivery of the protein. However, addition of N-glycans to GPI-anchored rGH resulted in predominant apical delivery, suggesting that N-glycans act as apical sorting signals on GPI-anchored proteins as they do on transmembrane and secretory proteins. In contrast to the GPI-anchored rGH, a transmembrane form of rGH which was not raft-associated accumulated intracellularly. Addition of N-glycans to this chimeric protein prevented intracellular accumulation and led to apical delivery.

Bibliography

Benting, J. H., Rietveld, A. G., & Simons, K. (1999). N-Glycans Mediate the Apical Sorting of a Gpi-Anchored, Raft-Associated Protein in Madin-Darby Canine Kidney Cells. The Journal of Cell Biology, 146(2), 313â320.

Authors 3

Jürgen H. Benting (first)
Anton G. Rietveld (additional)
Kai Simons (additional)

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Dates

Type	When
Created	23 years ago (July 26, 2002, 12:45 p.m.)
Deposited	2 years ago (July 28, 2023, 9:47 p.m.)
Indexed	1 month, 3 weeks ago (July 2, 2025, 2:31 p.m.)
Issued	26 years, 1 month ago (July 26, 1999)
Published	26 years, 1 month ago (July 26, 1999)
Published Online	26 years, 1 month ago (July 26, 1999)
Published Print	26 years, 1 month ago (July 26, 1999)

Funders 0

None

BibTeX

@article{Benting_1999, title={N-Glycans Mediate the Apical Sorting of a Gpi-Anchored, Raft-Associated Protein in Madin-Darby Canine Kidney Cells}, volume={146}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.146.2.313}, DOI={10.1083/jcb.146.2.313}, number={2}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Benting, Jürgen H. and Rietveld, Anton G. and Simons, Kai}, year={1999}, month=jul, pages={313–320} }

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