DOI: 10.1083/jcb.145.3.503. Abnormal Reaction to Central Nervous System Injury in Mice Lacking Glial Fibrillary Acidic Protein and Vimentin

Abnormal Reaction to Central Nervous System Injury in Mice Lacking Glial Fibrillary Acidic Protein and Vimentin

10.1083/jcb.145.3.503

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

In response to injury of the central nervous system, astrocytes become reactive and express high levels of the intermediate filament (IF) proteins glial fibrillary acidic protein (GFAP), vimentin, and nestin. We have shown that astrocytes in mice deficient for both GFAP and vimentin (GFAP−/−vim−/−) cannot form IFs even when nestin is expressed and are thus devoid of IFs in their reactive state. Here, we have studied the reaction to injury in the central nervous system in GFAP−/−, vimentin−/−, or GFAP−/−vim−/− mice. Glial scar formation appeared normal after spinal cord or brain lesions in GFAP−/− or vimentin−/− mice, but was impaired in GFAP−/−vim−/− mice that developed less dense scars frequently accompanied by bleeding. These results show that GFAP and vimentin are required for proper glial scar formation in the injured central nervous system and that some degree of functional overlap exists between these IF proteins.

Bibliography

Pekny, M., Johansson, C. B., Eliasson, C., Stakeberg, J., WallÃ©n, â«sa, Perlmann, T., Lendahl, U., Betsholtz, C., Berthold, C.-H., & FrisÃ©n, J. (1999). Abnormal Reaction to Central Nervous System Injury in Mice Lacking Glial Fibrillary Acidic Protein and Vimentin. The Journal of Cell Biology, 145(3), 503â514.

Authors 10

Milos Pekny (first)
Clas B. Johansson (additional)
Camilla Eliasson (additional)
Josefina Stakeberg (additional)
Åsa Wallén (additional)
Thomas Perlmann (additional)
Urban Lendahl (additional)
Christer Betsholtz (additional)
Claes-Henric Berthold (additional)
Jonas Frisén (additional)

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Dates

Type	When
Created	23 years ago (July 26, 2002, 12:45 p.m.)
Deposited	2 years ago (July 28, 2023, 8:45 p.m.)
Indexed	2 days, 22 hours ago (Aug. 23, 2025, 12:59 a.m.)
Issued	26 years, 3 months ago (May 3, 1999)
Published	26 years, 3 months ago (May 3, 1999)
Published Online	26 years, 3 months ago (May 3, 1999)
Published Print	26 years, 3 months ago (May 3, 1999)

Funders 0

None

BibTeX

@article{Pekny_1999, title={Abnormal Reaction to Central Nervous System Injury in Mice Lacking Glial Fibrillary Acidic Protein and Vimentin}, volume={145}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.145.3.503}, DOI={10.1083/jcb.145.3.503}, number={3}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Pekny, Milos and Johansson, Clas B. and Eliasson, Camilla and Stakeberg, Josefina and Wallén, Åsa and Perlmann, Thomas and Lendahl, Urban and Betsholtz, Christer and Berthold, Claes-Henric and Frisén, Jonas}, year={1999}, month=may, pages={503–514} }

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