DOI: 10.1083/jcb.145.1.141. The MAL Proteolipid Is Necessary for Normal Apical Transport and Accurate Sorting of the Influenza Virus Hemagglutinin in Madin-Darby Canine Kidney Cells

The MAL Proteolipid Is Necessary for Normal Apical Transport and Accurate Sorting of the Influenza Virus Hemagglutinin in Madin-Darby Canine Kidney Cells

10.1083/jcb.145.1.141

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

The MAL (MAL/VIP17) proteolipid is a nonglycosylated integral membrane protein expressed in a restricted pattern of cell types, including T lymphocytes, myelin-forming cells, and polarized epithelial cells. Transport of the influenza virus hemagglutinin (HA) to the apical surface of epithelial Madin-Darby canine kidney (MDCK) cells appears to be mediated by a pathway involving glycolipid- and cholesterol- enriched membranes (GEMs). In MDCK cells, MAL has been proposed previously as being an element of the protein machinery for the GEM-dependent apical transport pathway. Using an antisense oligonucleotide-based strategy and a newly generated monoclonal antibody to canine MAL, herein we have approached the effect of MAL depletion on HA transport in MDCK cells. We have found that MAL depletion diminishes the presence of HA in GEMs, reduces the rate of HA transport to the cell surface, inhibits the delivery of HA to the apical surface, and produces partial missorting of HA to the basolateral membrane. These effects were corrected by ectopic expression of MAL in MDCK cells whose endogenous MAL protein was depleted. Our results indicate that MAL is necessary for both normal apical transport and accurate sorting of HA.

Bibliography

Puertollano, R., MartÃn-Belmonte, F., MillÃ¡n, J., del Carmen de Marco, M., Albar, J. P., Kremer, L., & Alonso, M. A. (1999). The MAL Proteolipid Is Necessary for Normal Apical Transport and Accurate Sorting of the Influenza Virus Hemagglutinin in Madin-Darby Canine Kidney Cells. The Journal of Cell Biology, 145(1), 141â151.

Authors 7

Rosa Puertollano (first)
Fernando Martín-Belmonte (additional)
Jaime Millán (additional)
María del Carmen de Marco (additional)
Juan P. Albar (additional)
Leonor Kremer (additional)
Miguel A. Alonso (additional)

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 12:45 p.m.)
Deposited	2 years, 1 month ago (July 28, 2023, 8:21 p.m.)
Indexed	3 weeks, 4 days ago (Aug. 7, 2025, 4:51 a.m.)
Issued	26 years, 4 months ago (April 5, 1999)
Published	26 years, 4 months ago (April 5, 1999)
Published Online	26 years, 4 months ago (April 5, 1999)
Published Print	26 years, 4 months ago (April 5, 1999)

Funders 0

None

BibTeX

@article{Puertollano_1999, title={The MAL Proteolipid Is Necessary for Normal Apical Transport and Accurate Sorting of the Influenza Virus Hemagglutinin in Madin-Darby Canine Kidney Cells}, volume={145}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.145.1.141}, DOI={10.1083/jcb.145.1.141}, number={1}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Puertollano, Rosa and Martín-Belmonte, Fernando and Millán, Jaime and del Carmen de Marco, María and Albar, Juan P. and Kremer, Leonor and Alonso, Miguel A.}, year={1999}, month=apr, pages={141–151} }

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