DOI: 10.1083/jcb.144.6.1203. Novel Protein Kinases Ark1p and Prk1p Associate with and Regulate the Cortical Actin Cytoskeleton in Budding Yeast

Novel Protein Kinases Ark1p and Prk1p Associate with and Regulate the Cortical Actin Cytoskeleton in Budding Yeast

10.1083/jcb.144.6.1203

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

Ark1p (actin regulating kinase 1) was identified as a yeast protein that binds to Sla2p, an evolutionarily conserved cortical actin cytoskeleton protein. Ark1p and a second yeast protein, Prk1p, contain NH2-terminal kinase domains that are 70% identical. Together with six other putative kinases from a number of organisms, these proteins define a new protein kinase family that we have named the Ark family. Lack of both Ark1p and Prk1p resulted in the formation of large cytoplasmic actin clumps and severe defects in cell growth. These defects were rescued by wild-type, but not by kinase-dead versions of the proteins. Elevated levels of either Ark1p or Prk1p caused a number of actin and cell morphological defects that were not observed when the kinase-dead versions were overexpressed instead. Ark1p and Prk1p were shown to localize to actin cortical patches, making these two kinases the first signaling proteins demonstrated to be patch components. These results suggest that Ark1p and Prk1p may be downstream effectors of signaling pathways that control actin patch organization and function. Furthermore, results of double-mutant analyses suggest that Ark1p and Prk1p function in overlapping but distinct pathways that regulate the cortical actin cytoskeleton.

Bibliography

Cope, M. J. T. V., Yang, S., Shang, C., & Drubin, D. G. (1999). Novel Protein Kinases Ark1p and Prk1p Associate with and Regulate the Cortical Actin Cytoskeleton in Budding Yeast. The Journal of Cell Biology, 144(6), 1203â1218.

Authors 4

M.Jamie T.V. Cope (first)
Shirley Yang (additional)
Ching Shang (additional)
David G. Drubin (additional)

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 12:45 p.m.)
Deposited	2 years, 1 month ago (July 28, 2023, 8:24 p.m.)
Indexed	4 months, 3 weeks ago (April 8, 2025, 9:21 p.m.)
Issued	26 years, 5 months ago (March 22, 1999)
Published	26 years, 5 months ago (March 22, 1999)
Published Online	26 years, 5 months ago (March 22, 1999)
Published Print	26 years, 5 months ago (March 22, 1999)

Funders 0

None

BibTeX

@article{Cope_1999, title={Novel Protein Kinases Ark1p and Prk1p Associate with and Regulate the Cortical Actin Cytoskeleton in Budding Yeast}, volume={144}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.144.6.1203}, DOI={10.1083/jcb.144.6.1203}, number={6}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Cope, M.Jamie T.V. and Yang, Shirley and Shang, Ching and Drubin, David G.}, year={1999}, month=mar, pages={1203–1218} }

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