DOI: 10.1083/jcb.144.6.1135. Golgi Structure in Three Dimensions: Functional Insights from the Normal Rat Kidney Cell

Golgi Structure in Three Dimensions: Functional Insights from the Normal Rat Kidney Cell

10.1083/jcb.144.6.1135

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

Three-dimensional reconstructions of portions of the Golgi complex from cryofixed, freeze-substituted normal rat kidney cells have been made by dual-axis, high-voltage EM tomography at ∼7-nm resolution. The reconstruction shown here (∼1 × 1 × 4 μm3) contains two stacks of seven cisternae separated by a noncompact region across which bridges connect some cisternae at equivalent levels, but none at nonequivalent levels. The rest of the noncompact region is filled with both vesicles and polymorphic membranous elements. All cisternae are fenestrated and display coated buds. They all have about the same surface area, but they differ in volume by as much as 50%. The trans-most cisterna produces exclusively clathrin-coated buds, whereas the others display only nonclathrin coated buds. This finding challenges traditional views of where sorting occurs within the Golgi complex. Tubules with budding profiles extend from the margins of both cis and trans cisternae. They pass beyond neighboring cisternae, suggesting that these tubules contribute to traffic to and/or from the Golgi. Vesicle-filled “wells” open to both the cis and lateral sides of the stacks. The stacks of cisternae are positioned between two types of ER, cis and trans. The cis ER lies adjacent to the ER-Golgi intermediate compartment, which consists of discrete polymorphic membranous elements layered in front of the cis-most Golgi cisterna. The extensive trans ER forms close contacts with the two trans-most cisternae; this apposition may permit direct transfer of lipids between ER and Golgi membranes. Within 0.2 μm of the cisternae studied, there are 394 vesicles (8 clathrin coated, 190 nonclathrin coated, and 196 noncoated), indicating considerable vesicular traffic in this Golgi region. Our data place structural constraints on models of trafficking to, through, and from the Golgi complex.

Bibliography

Ladinsky, M. S., Mastronarde, D. N., McIntosh, J. R., Howell, K. E., & Staehelin, L. A. (1999). Golgi Structure in Three Dimensions: Functional Insights from the Normal Rat Kidney Cell. The Journal of Cell Biology, 144(6), 1135â1149.

Authors 5

Mark S. Ladinsky (first)
David N. Mastronarde (additional)
J. Richard McIntosh (additional)
Kathryn E. Howell (additional)
L. Andrew Staehelin (additional)

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Dates

Type	When
Created	23 years ago (July 26, 2002, 12:45 p.m.)
Deposited	2 years ago (July 28, 2023, 8:32 p.m.)
Indexed	21 hours, 12 minutes ago (Aug. 20, 2025, 8:22 a.m.)
Issued	26 years, 4 months ago (March 22, 1999)
Published	26 years, 4 months ago (March 22, 1999)
Published Online	26 years, 4 months ago (March 22, 1999)
Published Print	26 years, 4 months ago (March 22, 1999)

Funders 0

None

BibTeX

@article{Ladinsky_1999, title={Golgi Structure in Three Dimensions: Functional Insights from the Normal Rat Kidney Cell}, volume={144}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.144.6.1135}, DOI={10.1083/jcb.144.6.1135}, number={6}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Ladinsky, Mark S. and Mastronarde, David N. and McIntosh, J. Richard and Howell, Kathryn E. and Staehelin, L. Andrew}, year={1999}, month=mar, pages={1135–1149} }

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