DOI: 10.1083/jcb.144.5.857. Calreticulin Is Essential for Cardiac Development

Calreticulin Is Essential for Cardiac Development

10.1083/jcb.144.5.857

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

Calreticulin is a ubiquitous Ca2+ binding protein, located in the endoplasmic reticulum lumen, which has been implicated in many diverse functions including: regulation of intracellular Ca2+ homeostasis, chaperone activity, steroid-mediated gene regulation, and cell adhesion. To understand the physiological function of calreticulin we used gene targeting to create a knockout mouse for calreticulin. Mice homozygous for the calreticulin gene disruption developed omphalocele (failure of absorption of the umbilical hernia) and showed a marked decrease in ventricular wall thickness and deep intertrabecular recesses in the ventricular walls. Transgenic mice expressing a green fluorescent protein reporter gene under the control of the calreticulin promoter were used to show that the calreticulin gene is highly activated in the cardiovascular system during the early stages of cardiac development. Calreticulin protein is also highly expressed in the developing heart, but it is only a minor component of the mature heart. Bradykinin-induced Ca2+ release by the InsP3-dependent pathway was inhibited in crt−/− cells, suggesting that calreticulin plays a role in Ca2+ homeostasis. Calreticulin-deficient cells also exhibited impaired nuclear import of nuclear factor of activated T cell (NF-AT3) transcription factor indicating that calreticulin plays a role in cardiac development as a component of the Ca2+/calcineurin/NF-AT/GATA-4 transcription pathway.

Bibliography

Mesaeli, N., Nakamura, K., Zvaritch, E., Dickie, P., Dziak, E., Krause, K.-H., Opas, M., MacLennan, D. H., & Michalak, M. (1999). Calreticulin Is Essential for Cardiac Development. The Journal of Cell Biology, 144(5), 857â868.

Authors 9

Nasrin Mesaeli (first)
Kimitoshi Nakamura (additional)
Elena Zvaritch (additional)
Peter Dickie (additional)
Ewa Dziak (additional)
Karl-Heinz Krause (additional)
Michal Opas (additional)
David H. MacLennan (additional)
Marek Michalak (additional)

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 12:45 p.m.)
Deposited	2 years, 1 month ago (July 28, 2023, 8:29 p.m.)
Indexed	3 weeks, 5 days ago (Aug. 6, 2025, 8 a.m.)
Issued	26 years, 5 months ago (March 8, 1999)
Published	26 years, 5 months ago (March 8, 1999)
Published Online	26 years, 5 months ago (March 8, 1999)
Published Print	26 years, 5 months ago (March 8, 1999)

Funders 0

None

BibTeX

@article{Mesaeli_1999, title={Calreticulin Is Essential for Cardiac Development}, volume={144}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.144.5.857}, DOI={10.1083/jcb.144.5.857}, number={5}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Mesaeli, Nasrin and Nakamura, Kimitoshi and Zvaritch, Elena and Dickie, Peter and Dziak, Ewa and Krause, Karl-Heinz and Opas, Michal and MacLennan, David H. and Michalak, Marek}, year={1999}, month=mar, pages={857–868} }

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