DOI: 10.1083/jcb.144.1.31. Distinct Dynamin-dependent and -independent Mechanisms Target Structurally Homologous Dopamine Receptors to Different Endocytic Membranes

Distinct Dynamin-dependent and -independent Mechanisms Target Structurally Homologous Dopamine Receptors to Different Endocytic Membranes

10.1083/jcb.144.1.31

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

D1 and D2 dopamine receptors are structurally homologous G protein–coupled receptors that serve distinct physiological functions both in neurons and nonneural cell types. We have observed that these receptors are selectively endocytosed in HEK293 cells by distinct dynamin-dependent and -independent mechanisms. Although these endocytic mechanisms operate with similarly rapid kinetics, they differ in their regulation by agonist and deliver D1 and D2 receptors specifically to different primary endocytic vesicles. After this segregation into different endocytic membranes, both D1 and D2 receptors recycle to the plasma membrane. Similar results are observed in Neuro2A neuroblastoma cells coexpressing both receptors at high levels. These findings establish that “classical” dynamin-dependent and “alternative” dynamin-independent endocytic mechanisms differ in their physiological regulation, sort structurally homologous signaling receptors in the plasma membrane, and mediate distinct early endocytic pathways leading to recycling endosomes. Our results also refute the previous hypothesis that dynamin-independent endocytosis targets G protein–coupled receptors selectively to lysosomes, and they suggest a new role of endocytic sorting mechanisms in physically segregating structurally homologous signaling receptors at the cell surface.

Bibliography

Vickery, R. G., & von Zastrow, M. (1999). Distinct Dynamin-dependent and -independent Mechanisms Target Structurally Homologous Dopamine Receptors to Different Endocytic Membranes. The Journal of Cell Biology, 144(1), 31â43.

Authors 2

Ross G. Vickery (first)
Mark von Zastrow (additional)

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 12:45 p.m.)
Deposited	2 years ago (July 28, 2023, 8:06 p.m.)
Indexed	3 weeks, 5 days ago (Aug. 2, 2025, 1:20 a.m.)
Issued	26 years, 7 months ago (Jan. 11, 1999)
Published	26 years, 7 months ago (Jan. 11, 1999)
Published Online	26 years, 7 months ago (Jan. 11, 1999)
Published Print	26 years, 7 months ago (Jan. 11, 1999)

Funders 0

None

BibTeX

@article{Vickery_1999, title={Distinct Dynamin-dependent and -independent Mechanisms Target Structurally Homologous Dopamine Receptors to Different Endocytic Membranes}, volume={144}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.144.1.31}, DOI={10.1083/jcb.144.1.31}, number={1}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Vickery, Ross G. and von Zastrow, Mark}, year={1999}, month=jan, pages={31–43} }

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