DOI: 10.1083/jcb.144.1.125. The Bipolar Kinesin, KLP61F, Cross-links Microtubules within Interpolar Microtubule Bundles of <i>Drosophila</i> Embryonic Mitotic Spindles

The Bipolar Kinesin, KLP61F, Cross-links Microtubules within Interpolar Microtubule Bundles of Drosophila Embryonic Mitotic Spindles

10.1083/jcb.144.1.125

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

Previous genetic and biochemical studies have led to the hypothesis that the essential mitotic bipolar kinesin, KLP61F, cross-links and slides microtubules (MTs) during spindle assembly and function. Here, we have tested this hypothesis by immunofluorescence and immunoelectron microscopy (immunoEM). We show that Drosophila embryonic spindles at metaphase and anaphase contain abundant bundles of MTs running between the spindle poles. These interpolar MT bundles are parallel near the poles and antiparallel in the midzone. We have observed that KLP61F motors, phosphorylated at a cdk1/cyclin B consensus domain within the BimC box (BCB), localize along the length of these interpolar MT bundles, being concentrated in the midzone region. Nonphosphorylated KLP61F motors, in contrast, are excluded from the spindle and display a cytoplasmic localization. Immunoelectron microscopy further suggested that phospho-KLP61F motors form cross-links between MTs within interpolar MT bundles. These bipolar KLP61F MT-MT cross-links should be capable of organizing parallel MTs into bundles within half spindles and sliding antiparallel MTs apart in the spindle midzone. Thus we propose that bipolar kinesin motors and MTs interact by a “sliding filament mechanism” during the formation and function of the mitotic spindle.

Bibliography

Sharp, D. J., McDonald, K. L., Brown, H. M., Matthies, H. J., Walczak, C., Vale, R. D., Mitchison, T. J., & Scholey, J. M. (1999). The Bipolar Kinesin, KLP61F, Cross-links Microtubules within Interpolar Microtubule Bundles of Drosophila Embryonic Mitotic Spindles. The Journal of Cell Biology, 144(1), 125â138.

Authors 8

David J. Sharp (first)
Kent L. McDonald (additional)
Heather M. Brown (additional)
Heinrich J. Matthies (additional)
Claire Walczak (additional)
Ron D. Vale (additional)
Timothy J. Mitchison (additional)
Jonathan M. Scholey (additional)

References 46 Referenced 273

10.1242/jcs.104.4.1207 / J Cell Sci / Astral and spindle forces in PtK2 cells during anaphase B: a laser microbeam study by Aist (1993)
{'key': '2023072900001429800_B2'}
10.1091/mbc.6.11.1563 / Mol Biol Cell / Motor-activity and mitotic spindle localization of the Drosophilakinesin-like protein KLP61F by Barton (1995)
10.1073/pnas.93.5.1735 / Proc Natl Acad Sci USA / Going mobile: microtubule motors and chromosome segregation by Barton (1996)
10.1002/cm.970160204 / Cell Motil Cytoskel / Toward a structural and molecular definition of the kinetochore by Brinkley (1990)
10.1016/S0021-9258(17)31593-4 / J Biol Chem / A ‘slow' homotetrameric kinesin-related protein purified from Drosophilaembryos by Cole (1994)
10.1083/jcb.128.1.1 / J Cell Biol / A new role for motor proteins as couplers to depolymerizing microtubules by Desai (1995)
10.1242/jcs.111.7.853 / J Cell Sci / Mutations in the BCB of Cut7 indicate divergence of regulation within the bimC family of kinesin-related proteins by Drummond (1998)
10.1016/0092-8674(90)90350-N / Cell / Mutation of a gene that encodes a kinesin-like protein blocks nuclear division in Aspergillus nidulans. by Enos (1990)
10.1083/jcb.94.3.644 / J Cell Biol / Polarity of microtubules in Haemanthusendosperm by Euteneuer (1982)
10.1038/359480a0 / Nature / Every motion has its motor by Gelfand (1992)
10.1038/347563a0 / Nature / Novel potential mitotic motor protein encoded by the fission yeast Cut7+gene by Hagan (1990)
10.1038/356074a0 / Nature / Kinesin-related Cut7 protein associates with mitotic and meiotic spindles in fission yeast by Hagan (1992)
10.1083/jcb.123.3.665 / J Cell Biol / The kinesin-like protein KLP61F is essential for mitosis in Drosophila. by Heck (1993)
10.1016/0955-0674(94)90117-1 / Cur Opin Cell Biol / Cellular roles of kinesin and related proteins by Hoyt (1994)
10.1083/jcb.118.1.109 / J Cell Biol / Two Saccharomyces cerevisiaekinesin-related gene-products required for mitotic spindle assembly by Hoyt (1992)
10.1073/pnas.90.24.11653 / Proc Natl Acad Sci USA / Kinesin swivels for microtubule movement in any direction by Hunt (1993)
10.1038/379270a0 / Nature / A bipolar kinesin by Kashina (1996)
10.1038/384225a0 / Nature / An essential bipolar mitotic motor by Kashina (1996)
10.1016/S0167-4889(97)00037-2 / Biochim Biophys Acta / The bimC family of kinesins: essential bipolar mitotic motors driving centrosome separation by Kashina (1997)
{'key': '2023072900001429800_B21'}
{'key': '2023072900001429800_B22'}
10.1083/jcb.123.6.1475 / J Cell Biol / Interpolar spindle microtubules in PTK cells by Mastronarde (1993)
10.1016/S0091-679X(08)60926-7 / Methods Cell Biol / Electron microscopy and EM immunocytochemistry by McDonald (1994)
10.1083/jcb.74.2.377 / J Cell Biol / On the mechanism of anaphase spindle elongation in Diatoma vulgare. by McDonald (1977)
10.1038/224659a0 / Nature / Model for mitosis by McIntosh (1969)
10.1083/jcb.49.2.468 / J Cell Biol / The distribution of spindle microtubules during mitosis in cultured human cells by McIntosh (1971)
10.1016/S0076-6879(98)98015-6 / Methods Enzymol / Purification of novel kinesins from embryonic systems by Meyer (1998)
10.1083/jcb.120.1.153 / J Cell Biol / Suppression of the bimC mitotic spindle defect by deletion of klpA, a kar3 related kinesin like protein in Aspergillus nidulans. by O'Connell (1993)
10.1083/jcb.131.5.1261 / J Cell Biol / The cell cycle-dependent localization of the CP190 centrosomal protein is determined by the coordinate action of two separable domains by Oegema (1995)
10.1091/mbc.7.10.1639 / Mol Biol Cell / Fission yeast pkl1 is a kinesin-related protein involved in mitotic spindle function by Pidoux (1996)
10.1083/jcb.118.1.95 / J Cell Biol / Kinesin-related proteins required for assembly of the mitotic spindle by Roof (1992)
10.1016/0092-8674(92)90169-D / Cell / Kinesin-related proteins required for structural integrity of the mitotic spindle by Saunders (1992)
10.1083/jcb.128.4.617 / J Cell Biol / Saccharomyces cerevisiaekinesin- and dynein-related proteins required for anaphase chromosome segregation by Saunders (1995)
10.1083/jcb.137.2.417 / J Cell Biol / The Saccharomyces cerevisiaekinesin-related motor Kar3p acts at preanaphase spindle poles to limit the number and length of cytoplasmic microtubules by Saunders (1997)
10.1091/mbc.8.6.1025 / Molecular Biol Cell / Mitotic spindle function in Saccharomyces cerevisiaerequires a balance between different types of kinesin-related motors by Saunders (1997)
10.1038/359540a0 / Nature / Mitotic spindle organization by a plus-end-directed microtubule motor by Sawin (1992)
10.1073/pnas.92.10.4289 / Proc Nat Acad Sci USA / Mutations in the kinesin-like protein Eg5 disrupting localization to the mitotic spindle by Sawin (1995)
{'key': '2023072900001429800_B39', 'first-page': '179', 'article-title': 'Dynamics of the nuclear envelope and of nuclear pore complexes during mitosis in the Drosophilaembryo', 'volume': '34', 'author': 'Stafstrom', 'year': '1984', 'journal-title': 'Eur J Cell Biol'} / Eur J Cell Biol / Dynamics of the nuclear envelope and of nuclear pore complexes during mitosis in the Drosophilaembryo by Stafstrom (1984)
{'key': '2023072900001429800_B40'}
{'key': '2023072900001429800_B41'}
10.1083/jcb.143.3.687 / J Cell Biol / Time-lapse microscopy reveals unique roles for kinesins during anaphase in budding yeast by Straight (1998)
10.1016/S0091-679X(08)60928-0 / Methods Cell Biol / Immunofluorescence analysis of the cytoskeleton during oogenesis and early embryogenesis by Theurkauf (1994)
10.1016/S0092-8674(00)81295-7 / Cell / Kinesin-related proteins at mitotic spindle poles-function and regulation by Walczak (1996)
10.1083/jcb.129.6.1601 / J Cell Biol / Three-dimensional ultrastructural analysis of the Saccharomyces cerevisiaemitotic spindle by Winey (1995)
10.1083/jcb.123.3.681 / J Cell Biol / Roles of kinesin and kinesin-like proteins in sea urchin embryonic cell division: evaluation using antibody microinjection by Wright (1993)

Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 12:45 p.m.)
Deposited	2 years, 1 month ago (July 28, 2023, 8:01 p.m.)
Indexed	2 months ago (June 27, 2025, 10:24 a.m.)
Issued	26 years, 7 months ago (Jan. 11, 1999)
Published	26 years, 7 months ago (Jan. 11, 1999)
Published Online	26 years, 7 months ago (Jan. 11, 1999)
Published Print	26 years, 7 months ago (Jan. 11, 1999)

Funders 0

None

BibTeX

@article{Sharp_1999, title={The Bipolar Kinesin, KLP61F, Cross-links Microtubules within Interpolar Microtubule Bundles of Drosophila Embryonic Mitotic Spindles}, volume={144}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.144.1.125}, DOI={10.1083/jcb.144.1.125}, number={1}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Sharp, David J. and McDonald, Kent L. and Brown, Heather M. and Matthies, Heinrich J. and Walczak, Claire and Vale, Ron D. and Mitchison, Timothy J. and Scholey, Jonathan M.}, year={1999}, month=jan, pages={125–138} }

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      "volume": "104",
      "author": "Aist",
      "year": "1993",
      "journal-title": "J Cell Sci"
    },
    {
      "key": "2023072900001429800_B2"
    },
    {
      "key": "2023072900001429800_B3",
      "doi-asserted-by": "crossref",
      "first-page": "1563",
      "DOI": "10.1091/mbc.6.11.1563",
      "article-title": "Motor-activity and mitotic spindle localization of the Drosophilakinesin-like protein KLP61F",
      "volume": "6",
      "author": "Barton",
      "year": "1995",
      "journal-title": "Mol Biol Cell"
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      "doi-asserted-by": "crossref",
      "first-page": "1735",
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      "article-title": "Going mobile: microtubule motors and chromosome segregation",
      "volume": "5",
      "author": "Barton",
      "year": "1996",
      "journal-title": "Proc Natl Acad Sci USA"
    },
    {
      "key": "2023072900001429800_B5",
      "doi-asserted-by": "crossref",
      "first-page": "104",
      "DOI": "10.1002/cm.970160204",
      "article-title": "Toward a structural and molecular definition of the kinetochore",
      "volume": "16",
      "author": "Brinkley",
      "year": "1990",
      "journal-title": "Cell Motil Cytoskel"
    },
    {
      "key": "2023072900001429800_B6",
      "doi-asserted-by": "crossref",
      "first-page": "22913",
      "DOI": "10.1016/S0021-9258(17)31593-4",
      "article-title": "A \u2018slow' homotetrameric kinesin-related protein purified from Drosophilaembryos",
      "volume": "269",
      "author": "Cole",
      "year": "1994",
      "journal-title": "J Biol Chem"
    },
    {
      "key": "2023072900001429800_B7",
      "doi-asserted-by": "crossref",
      "first-page": "1",
      "DOI": "10.1083/jcb.128.1.1",
      "article-title": "A new role for motor proteins as couplers to depolymerizing microtubules",
      "volume": "128",
      "author": "Desai",
      "year": "1995",
      "journal-title": "J Cell Biol"
    },
    {
      "key": "2023072900001429800_B8",
      "doi-asserted-by": "crossref",
      "first-page": "853",
      "DOI": "10.1242/jcs.111.7.853",
      "article-title": "Mutations in the BCB of Cut7 indicate divergence of regulation within the bimC family of kinesin-related proteins",
      "volume": "111",
      "author": "Drummond",
      "year": "1998",
      "journal-title": "J Cell Sci"
    },
    {
      "key": "2023072900001429800_B9",
      "doi-asserted-by": "crossref",
      "first-page": "1019",
      "DOI": "10.1016/0092-8674(90)90350-N",
      "article-title": "Mutation of a gene that encodes a kinesin-like protein blocks nuclear division in Aspergillus nidulans.",
      "volume": "60",
      "author": "Enos",
      "year": "1990",
      "journal-title": "Cell"
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    {
      "key": "2023072900001429800_B10",
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      "first-page": "644",
      "DOI": "10.1083/jcb.94.3.644",
      "article-title": "Polarity of microtubules in Haemanthusendosperm",
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      "author": "Euteneuer",
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      "journal-title": "Methods Cell Biol"
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