DOI: 10.1083/jcb.143.7.1931. Tropomyosin-containing Actin Cables Direct the Myo2p-dependent Polarized Delivery of Secretory Vesicles in Budding Yeast

Tropomyosin-containing Actin Cables Direct the Myo2p-dependent Polarized Delivery of Secretory Vesicles in Budding Yeast

10.1083/jcb.143.7.1931

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

The actin cytoskeleton in budding yeast consists of cortical patches and cables, both of which polarize toward regions of cell growth. Tropomyosin localizes specifically to actin cables and not cortical patches. Upon shifting cells with conditionally defective tropomyosin to restrictive temperatures, actin cables disappear within 1 min and both the unconventional class V myosin Myo2p and the secretory vesicle–associated Rab GTPase Sec4p depolarize rapidly. Bud growth ceases and the mother cell grows isotropically. When returned to permissive temperatures, tropomyosin-containing cables reform within 1 min in polarized arrays. Cable reassembly permits rapid enrichment of Myo2p at the focus of nascent cables as well as the Myo2p-dependent recruitment of Sec4p and the exocyst protein Sec8p, and the initiation of bud emergence. With the loss of actin cables, cortical patches slowly assume an isotropic distribution within the cell and will repolarize only after restoration of cables. Therefore, actin cables respond to polarity cues independently of the overall distribution of cortical patches and are able to directly target the Myo2p-dependent delivery of secretory vesicles and polarization of growth.

Bibliography

Pruyne, D. W., Schott, D. H., & Bretscher, A. (1998). Tropomyosin-containing Actin Cables Direct the Myo2p-dependent Polarized Delivery of Secretory Vesicles in Budding Yeast. The Journal of Cell Biology, 143(7), 1931â1945.

Authors 3

David W. Pruyne (first)
Daniel H. Schott (additional)
Anthony Bretscher (additional)

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 12:45 p.m.)
Deposited	2 years, 1 month ago (July 28, 2023, 8:49 p.m.)
Indexed	4 days, 11 hours ago (Aug. 27, 2025, 11:34 a.m.)
Issued	26 years, 9 months ago (Nov. 28, 1998)
Published	26 years, 9 months ago (Nov. 28, 1998)
Published Online	26 years, 9 months ago (Nov. 28, 1998)
Published Print	26 years, 8 months ago (Dec. 28, 1998)

Funders 0

None

BibTeX

@article{Pruyne_1998, title={Tropomyosin-containing Actin Cables Direct the Myo2p-dependent Polarized Delivery of Secretory Vesicles in Budding Yeast}, volume={143}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.143.7.1931}, DOI={10.1083/jcb.143.7.1931}, number={7}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Pruyne, David W. and Schott, Daniel H. and Bretscher, Anthony}, year={1998}, month=nov, pages={1931–1945} }

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