DOI: 10.1083/jcb.143.6.1559. Role of Xklp3, a Subunit of the <i>Xenopus</i> Kinesin II Heterotrimeric Complex, in Membrane Transport between the Endoplasmic Reticulum and the Golgi Apparatus

Role of Xklp3, a Subunit of the Xenopus Kinesin II Heterotrimeric Complex, in Membrane Transport between the Endoplasmic Reticulum and the Golgi Apparatus

10.1083/jcb.143.6.1559

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

The function of the Golgi apparatus is to modify proteins and lipids synthesized in the ER and sort them to their final destination. The steady-state size and function of the Golgi apparatus is maintained through the recycling of some components back to the ER. Several lines of evidence indicate that the spatial segregation between the ER and the Golgi apparatus as well as trafficking between these two compartments require both microtubules and motors. We have cloned and characterized a new Xenopus kinesin like protein, Xklp3, a subunit of the heterotrimeric Kinesin II. By immunofluorescence it is found in the Golgi region. A more detailed analysis by EM shows that it is associated with a subset of membranes that contain the KDEL receptor and are localized between the ER and Golgi apparatus. An association of Xklp3 with the recycling compartment is further supported by a biochemical analysis and the behavior of Xklp3 in BFA-treated cells. The function of Xklp3 was analyzed by transfecting cells with a dominant-negative form lacking the motor domain. In these cells, the normal delivery of newly synthesized proteins to the Golgi apparatus is blocked. Taken together, these results indicate that Xklp3 is involved in the transport of tubular-vesicular elements between the ER and the Golgi apparatus.

Bibliography

Le Bot, N., Antony, C., White, J., Karsenti, E., & Vernos, I. (1998). Role of Xklp3, a Subunit of the Xenopus Kinesin II Heterotrimeric Complex, in Membrane Transport between the Endoplasmic Reticulum and the Golgi Apparatus. The Journal of Cell Biology, 143(6), 1559â1573.

Authors 5

Nathalie Le Bot (first)
Claude Antony (additional)
Jamie White (additional)
Eric Karsenti (additional)
Isabelle Vernos (additional)

References 60 Referenced 80

10.1016/0092-8674(84)90195-8 / Cell / Direct visualization of protein transport and processing in the living cell by microinjection of specific antibodies by Arnheiter (1984)
{'key': '2023072900482270900_B2'}
10.1016/S0092-8674(00)80992-7 / Cell / Xklp2, a novel Xenopuscentrosomal kinesin-like protein required for centrosome separation during mitosis by Boleti (1996)
10.1083/jcb.139.2.469 / J Cell Biol / Overexpression of the dynamitin (p50) subunit of the dynactin complex disrupts dynein-dependent maintenance of membrane organelle distribution by Burkhardt (1997)
10.1038/366268a0 / Nature / Novel heterotrimeric kinesin-related protein purified from sea urchin eggs by Cole (1993)
10.1083/jcb.141.4.993 / J Cell Biol / Chlamydomonas kinesin-II-dependent intraflagellar transport (IFT): IFT particles contain proteins required for ciliary assembly in Caenorhabditis eleganssensory neurons by Cole (1998)
10.1083/jcb.118.6.1333 / J Cell Biol / Cytoplasmic dynein participates in the centrosomal localization of the Golgi complex by Corthesy-Theulaz (1992)
10.1083/jcb.109.1.61 / J Cell Biol / Brefeldin A redistributes resident and itinerant Golgi proteins to the endoplasmic reticulum by Doms (1989)
10.1074/jbc.273.32.20267 / J Biol Chem / Characterization of KIF1C, a new kinesin-like protein involved in vesicle transport form the Golgi apparatus to the endoplasmic reticulum by Dorner (1998)
10.1126/science.279.5350.580 / Science / Interaction of a Golgi-associated kinesin-like protein with Rab6 by Echard (1998)
10.1083/jcb.127.4.1021 / J Cell Biol / Kinesin-mediated organelle translocation revealed by specific cellular manipulations by Feiguin (1994)
10.1016/S0014-5793(97)00097-5 / FEBS (Fed Eur Biochem Soc) Lett / Characterization of brefeldin A induced vesicular structures containing cycling proteins of the intermediate compartment/cis-Golgi network by Fullekrüg (1997)
10.1083/jcb.127.6.1557 / J Cell Biol / Localization of the Lys, Asp, Glu, Leu tetrapeptide receptor to the Golgi complex and the intermediate compartment in mammalian cells by Griffiths (1994)
10.1083/jcb.141.1.51 / J Cell Biol / Golgi vesiculation and lysosome dispersion in cells lacking cytoplasmic dynein by Harada (1998)
{'key': '2023072900482270900_B15'}
10.1006/dbio.1995.1270 / Dev Biol / Immunolocalization of the heterotrimeric kinesin-related protein KRP(85/95) in the mitotic apparatus of sea urchin embryos by Henson (1995)
10.1083/jcb.125.5.1095 / J Cell Biol / KIF3A is a new microtubule-based anterograde motor in the nerve axon by Kondo (1994)
10.1083/jcb.131.6.1517 / J Cell Biol / The Chlamydomonaskinesin-like protein FLA10 is involved in motility associated with the flagellar membrane by Kozminski (1995)
10.1016/0092-8674(86)90075-9 / Cell / Oligomerization is essential for transport of vesicular stomatitis viral glycoprotein to the cell surface by Kreis (1986)
10.1016/0092-8674(92)90476-S / Cell / Ligand-induced redistribution of a human KDEL receptor from the Golgi complex to the endoplasmic reticulum by Lewis (1992)
10.1007/BF02473201 / Histochem Cell Biol / Improving structural integrity of cryosections for immunogold labeling by Liou (1996)
10.1016/S0955-0674(98)80086-0 / Curr Opin Cell Biol / Cytoskeletal proteins and Golgi dynamics by Lippincott-Schwartz (1998)
10.1016/S0962-8924(97)01199-9 / Trends Cell Biol / Unravelling Golgi membrane traffic with green fluorescent protein chimeras by Lippincott-Schwartz (1998)
10.1083/jcb.128.3.293 / J Cell Biol / Kinesin is the motor for microtubule-mediated Golgi-to-ER membrane traffic by Lippincott-Schwartz (1995)
10.1126/science.252.5009.1162 / Science / Predicting coiled coils from protein sequences by Lupas (1991)
10.1007/BF02627851 / In Vitro / Comparison of in vivo and in vitro ribosomal RNA synthesis in nucleolar mutants of Xenopus laevis. by Miller (1977)
10.1083/jcb.138.5.1009 / J Cell Biol / Heterotrimeric kinesin II is required for the assembly of motile 9+2 ciliary axonemes on sea urchin embryos by Morris (1997)
{'key': '2023072900482270900_B28'}
10.1016/0092-8674(89)90105-0 / Cell / Short cytoplasmic sequences serve as retention signals for transmembrane proteins in the endoplasmic reticulum by Nilsson (1989)
10.1177/33.9.4020101 / J Histochem Cytochem / Localization of binding sites for concanavalin A, Ricinus communis Iand Helix pomatia lectin in the Golgi apparatus of rat small intestinal absorptive cells by Pavelka (1985)
10.1083/jcb.141.4.979 / J Cell Biol / A dynein light chain is essential for the retrograde particle movement of intraflagellar transport (IFT) by Pazour (1998)
10.1083/jcb.127.4.1041 / J Cell Biol / Characterization of the KLP68D kinesin-like protein in Drosophila: possible roles in axonal transport by Pesavento (1994)
10.1038/38001 / Nature / ER-to-Golgi transport visualized in living cells by Presley (1997)
10.1159/000130054 / Cytogenetics / The length of secondary chromosomal constrictions in normal individuals and in a nucleolar mutant of Xenopus laevis. by Rafferty (1969)
{'key': '2023072900482270900_B35'}
10.1006/jmbi.1995.0484 / J Mol Biol / Heterodimerization of the two motor subunits of the heterotrimeric kinesin, KRP85/95 by Rashid (1995)
10.1016/S0960-9822(97)70092-7 / Curr Biol / Movement of nuclei along microtubules in Xenopusegg extracts by Reinsch (1997)
10.1073/pnas.94.8.3720 / Proc Natl Acad Sci USA / Regulated bidirectional motility of melanophore pigment granules along microtubules in vitro by Rogers (1997)
10.1083/jcb.98.2.399 / J Cell Biol / Cytochemical localization of terminal N-acetyl-d-galactosamine residues in cellular compartments of intestinal goblet cells: implications for the topology of O-glycosylation by Roth (1984)
10.1083/jcb.137.2.319 / J Cell Biol / Dissociation of coatomer from membranes is required for brefeldin A-induced transfer of Golgi enzymes to the endoplasmic reticulum by Scheel (1997)
10.1083/jcb.133.1.1 / J Cell Biol / Kinesin II, a membrane traffic motor in axons, axonemes, and spindles by Scholey (1996)
10.1083/jcb.139.5.1137 / J Cell Biol / Golgi tubule traffic and the effects of brefeldin A visualized in living cells by Sciaky (1997)
10.1083/jcb.137.6.1211 / JCell Biol / Partitioning of the Golgi apparatus during mitosis in living HeLa cells by Shima (1997)
10.1074/jbc.271.43.27013 / J Biol Chem / SMAP, a Smg GDS-associating protein having Arm repeats and phosphorylated by Src tyrosine kinase by Shimizu (1996)
10.1083/jcb.113.1.123 / J Cell Biol / Immunolocalization of the insulin regulatable glucose transporter in brown adipose tissue of the rat by Slot (1991)
10.1016/0378-1119(88)90005-4 / Gene / Single step purification of polypeptides expressed in Escherichia colias fusions with glutathione S-transferase by Smith (1988)
10.1006/jmbi.1994.0146 / J Mol Biol / Exclusive expression of C. elegansosm-3 kinesin gene in chemosensory neurons open to the external environment by Tabish (1995)
{'key': '2023072900482270900_B48', 'first-page': '199', 'article-title': 'Differential response of resident proteins and cycling proteins of the Golgi to brefeldin A', 'volume': '68', 'author': 'Tang', 'year': '1995', 'journal-title': 'Eur J Cell Biol'} / Eur J Cell Biol / Differential response of resident proteins and cycling proteins of the Golgi to brefeldin A by Tang (1995)
10.1083/jcb.120.2.325 / J Cell Biol / Molecular cloning, characterization, subcellular localization and dynamics of p23, the mammalian KDEL receptor by Tang (1993)
10.1083/jcb.109.1.35 / J Cell Biol / Condensation-sorting events in the rough endoplasmic reticulum of exocrine pancreatic cells by Tooze (1989)
{'key': '2023072900482270900_B51'}
10.1242/jcs.109.3.541 / J Cell Sci / The kinesin-homologous protein encoded by the Chlamydomonas FLA10 gene is associated with basal bodies and centrioles by Vashishtha (1996)
10.1006/dbio.1993.1127 / Dev Biol / Multiple kinesin-like transcripts in Xenopusoocytes by Vernos (1993)
10.1016/0092-8674(95)90376-3 / Cell / Xklp1, a chromosomal Xenopuskinesin-like protein essential for spindle organization and chromosome positioning by Vernos (1995)
10.1016/S0092-8674(00)80991-5 / Cell / XKCM1: a Xenopuskinesin-related protein that regulates microtubule dynamics during mitotic spindle assembly by Walczak (1996)
10.1083/jcb.132.3.371 / J Cell Biol / Sequence and submolecular localization of the 115-kD accessory subunit of the heterotrimeric kinesin-II (KRP85/95) complex by Wedaman (1996)
{'key': '2023072900482270900_B57'}
10.1083/jcb.130.6.1387 / J Cell Biol / KIF3A/B: a heterodimeric kinesin superfamily protein that works as a microtubule plus end-directed motor for membrane organelle transport by Yamazaki (1995)
10.1073/pnas.93.16.8443 / Proc Natl Acad Sci USA / Cloning and characterization of KAP3: a novel kinesin superfamily-associated protein of KIF3A/3B by Yamazaki (1996)
10.1091/mbc.9.2.249 / Mol Biol Cell / Characterization of the KIF3C neural kinesin-like motor from mouse by Yang (1998)

Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 12:45 p.m.)
Deposited	2 years, 1 month ago (July 28, 2023, 8:49 p.m.)
Indexed	1 month, 3 weeks ago (July 7, 2025, 2:02 a.m.)
Issued	26 years, 8 months ago (Dec. 14, 1998)
Published	26 years, 8 months ago (Dec. 14, 1998)
Published Online	26 years, 8 months ago (Dec. 14, 1998)
Published Print	26 years, 8 months ago (Dec. 14, 1998)

Funders 0

None

BibTeX

@article{Le_Bot_1998, title={Role of Xklp3, a Subunit of the Xenopus Kinesin II Heterotrimeric Complex, in Membrane Transport between the Endoplasmic Reticulum and the Golgi Apparatus}, volume={143}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.143.6.1559}, DOI={10.1083/jcb.143.6.1559}, number={6}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Le Bot, Nathalie and Antony, Claude and White, Jamie and Karsenti, Eric and Vernos, Isabelle}, year={1998}, month=dec, pages={1559–1573} }

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      "author": "Lippincott-Schwartz",
      "year": "1998",
      "journal-title": "Trends Cell Biol"
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      "first-page": "293",
      "DOI": "10.1083/jcb.128.3.293",
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      "volume": "128",
      "author": "Lippincott-Schwartz",
      "year": "1995",
      "journal-title": "J Cell Biol"
    },
    {
      "key": "2023072900482270900_B25",
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      "first-page": "1162",
      "DOI": "10.1126/science.252.5009.1162",
      "article-title": "Predicting coiled coils from protein sequences",
      "volume": "252",
      "author": "Lupas",
      "year": "1991",
      "journal-title": "Science"
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      "key": "2023072900482270900_B26",
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      "first-page": "557",
      "DOI": "10.1007/BF02627851",
      "article-title": "Comparison of in vivo and in vitro ribosomal RNA synthesis in nucleolar mutants of Xenopus laevis.",
      "volume": "13",
      "author": "Miller",
      "year": "1977",
      "journal-title": "In Vitro"
    },
    {
      "key": "2023072900482270900_B27",
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      "first-page": "1009",
      "DOI": "10.1083/jcb.138.5.1009",
      "article-title": "Heterotrimeric kinesin II is required for the assembly of motile 9+2 ciliary axonemes on sea urchin embryos",
      "volume": "138",
      "author": "Morris",
      "year": "1997",
      "journal-title": "J Cell Biol"
    },
    {
      "key": "2023072900482270900_B28"
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      "key": "2023072900482270900_B29",
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      "DOI": "10.1016/0092-8674(89)90105-0",
      "article-title": "Short cytoplasmic sequences serve as retention signals for transmembrane proteins in the endoplasmic reticulum",
      "volume": "58",
      "author": "Nilsson",
      "year": "1989",
      "journal-title": "Cell"
    },
    {
      "key": "2023072900482270900_B30",
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      "first-page": "905",
      "DOI": "10.1177/33.9.4020101",
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      "volume": "33",
      "author": "Pavelka",
      "year": "1985",
      "journal-title": "J Histochem Cytochem"
    },
    {
      "key": "2023072900482270900_B31",
      "doi-asserted-by": "crossref",
      "first-page": "979",
      "DOI": "10.1083/jcb.141.4.979",
      "article-title": "A dynein light chain is essential for the retrograde particle movement of intraflagellar transport (IFT)",
      "volume": "141",
      "author": "Pazour",
      "year": "1998",
      "journal-title": "J Cell Biol"
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    {
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      "doi-asserted-by": "crossref",
      "first-page": "1041",
      "DOI": "10.1083/jcb.127.4.1041",
      "article-title": "Characterization of the KLP68D kinesin-like protein in Drosophila: possible roles in axonal transport",
      "volume": "127",
      "author": "Pesavento",
      "year": "1994",
      "journal-title": "J Cell Biol"
    },
    {
      "key": "2023072900482270900_B33",
      "doi-asserted-by": "crossref",
      "first-page": "81",
      "DOI": "10.1038/38001",
      "article-title": "ER-to-Golgi transport visualized in living cells",
      "volume": "389",
      "author": "Presley",
      "year": "1997",
      "journal-title": "Nature"
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    {
      "key": "2023072900482270900_B34",
      "doi-asserted-by": "crossref",
      "first-page": "427",
      "DOI": "10.1159/000130054",
      "article-title": "The length of secondary chromosomal constrictions in normal individuals and in a nucleolar mutant of Xenopus laevis.",
      "volume": "8",
      "author": "Rafferty",
      "year": "1969",
      "journal-title": "Cytogenetics"
    },
    {
      "key": "2023072900482270900_B35"
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    {
      "key": "2023072900482270900_B36",
      "doi-asserted-by": "crossref",
      "first-page": "157",
      "DOI": "10.1006/jmbi.1995.0484",
      "article-title": "Heterodimerization of the two motor subunits of the heterotrimeric kinesin, KRP85/95",
      "volume": "252",
      "author": "Rashid",
      "year": "1995",
      "journal-title": "J Mol Biol"
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    {
      "key": "2023072900482270900_B37",
      "doi-asserted-by": "crossref",
      "first-page": "211",
      "DOI": "10.1016/S0960-9822(97)70092-7",
      "article-title": "Movement of nuclei along microtubules in Xenopusegg extracts",
      "volume": "7",
      "author": "Reinsch",
      "year": "1997",
      "journal-title": "Curr Biol"
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    {
      "key": "2023072900482270900_B38",
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      "first-page": "3720",
      "DOI": "10.1073/pnas.94.8.3720",
      "article-title": "Regulated bidirectional motility of melanophore pigment granules along microtubules in vitro",
      "volume": "94",
      "author": "Rogers",
      "year": "1997",
      "journal-title": "Proc Natl Acad Sci USA"
    },
    {
      "key": "2023072900482270900_B39",
      "doi-asserted-by": "crossref",
      "first-page": "399",
      "DOI": "10.1083/jcb.98.2.399",
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      "volume": "98",
      "author": "Roth",
      "year": "1984",
      "journal-title": "J Cell Biol"
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    {
      "key": "2023072900482270900_B40",
      "doi-asserted-by": "crossref",
      "first-page": "319",
      "DOI": "10.1083/jcb.137.2.319",
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      "volume": "137",
      "author": "Scheel",
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      "first-page": "1",
      "DOI": "10.1083/jcb.133.1.1",
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      "volume": "133",
      "author": "Scholey",
      "year": "1996",
      "journal-title": "J Cell Biol"
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      "first-page": "1137",
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      "author": "Sciaky",
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      "author": "Shima",
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      "volume": "271",
      "author": "Shimizu",
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      "volume": "113",
      "author": "Slot",
      "year": "1991",
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      "volume": "67",
      "author": "Smith",
      "year": "1988",
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      "volume": "247",
      "author": "Tabish",
      "year": "1995",
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      "author": "Tang",
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      "volume": "120",
      "author": "Tang",
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      "author": "Tooze",
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      "author": "Vashishtha",
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