DOI: 10.1083/jcb.143.3.719. Role of the Yeast Gin4p Protein Kinase in Septin Assembly and the Relationship between Septin Assembly and Septin Function

Role of the Yeast Gin4p Protein Kinase in Septin Assembly and the Relationship between Septin Assembly and Septin Function

10.1083/jcb.143.3.719

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

To identify septin-interacting proteins in Saccharomyces cerevisiae, we screened for mutations that are synthetically lethal with a cdc12 septin mutation. One of the genes identified was GIN4, which encodes a protein kinase related to Hsl1p/Nik1p and Ycl024Wp in S. cerevisiae and to Nim1p/Cdr1p and Cdr2p in Schizosaccharomyces pombe. The Gin4p kinase domain displayed a two-hybrid interaction with the COOH-terminal portion of the Cdc3p septin, and Gin4p colocalized with the septins at the mother–bud neck. This localization depended on the septins and on the COOH-terminal (nonkinase) region of Gin4p, and overproduction of this COOH-terminal region led to a loss of septin organization and associated morphogenetic defects. We detected no effect of deleting YCL024W, either alone or in combination with deletion of GIN4. Deletion of GIN4 was not lethal but led to a striking reorganization of the septins accompanied by morphogenetic abnormalities and a defect in cell separation; however, remarkably, cytokinesis appeared to occur efficiently. Two other proteins that localize to the neck in a septin-dependent manner showed similar reorganizations and also appeared to remain largely functional. The septin organization observed in gin4Δ vegetative cells resembles that seen normally in cells responding to mating pheromone, and no Gin4p was detected in association with the septins in such cells. The organization of the septins observed in gin4Δ cells and in cells responding to pheromone appears to support some aspects of the model for septin organization suggested previously by Field et al. (Field, C.M., O. Al-Awar, J. Rosenblatt, M.L. Wong, B. Alberts, and T.J. Mitchison. 1996. J. Cell Biol. 133:605–616).

Bibliography

Longtine, M. S., Fares, H., & Pringle, J. R. (1998). Role of the Yeast Gin4p Protein Kinase in Septin Assembly and the Relationship between Septin Assembly and Septin Function. The Journal of Cell Biology, 143(3), 719â736.

Authors 3

Mark S. Longtine (first)
Hanna Fares (additional)
John R. Pringle (additional)

References 72 Referenced 190

10.1126/science.270.5235.464 / Science / Role of yeast insulin-degrading enzyme homologs in propheromone processing and bud site selection by Adames (1995)
10.1083/jcb.98.3.934 / J Cell Biol / Relationship of actin and tubulin distribution to bud growth in wild-type and morphogenetic-mutant Saccharomyces cerevisiae. by Adams (1984)
10.1007/s004380050415 / Mol Gen Genet / Screening and identification of yeast sequences that cause growth inhibition when overexpressed by Akada (1997)
10.1083/jcb.138.1.119 / J Cell Biol / Control of mitotic events by Nap1 and the Gin4 kinase by Altman (1997)
{'key': '2023072901053561700_B5'}
10.1083/jcb.137.2.399 / J Cell Biol / High rates of actin filament turnover in budding yeast and roles for actin in establishment and maintenance of cell polarity revealed using the actin inhibitor latrunculin-A by Ayscough (1997)
10.1128/MMBR.62.2.249-274.1998 / Microbiol Mol Biol Rev / Signalling in the yeasts: an informational cascade with links to the filamentous fungi by Banuett (1998)
10.1093/nar/21.14.3329 / Nucleic Acids Res / A simple and efficient method for direct gene deletion in Saccharomyces cerevisiae. by Baudin (1993)
10.1128/MCB.17.9.5067 / Mol Cell Biol / Cla4p, a Saccharomyces cerevisiaeCdc42p-activated kinase involved in cytokinesis, is activated at mitosis by Benton (1997)
10.1128/MCB.16.10.5264 / Mol Cell Biol / ZDS1 and ZDS2, genes whose products may regulate Cdc42p in Saccharomyces cerevisiae. by Bi (1996)
10.1083/jcb.142.5.1301 / J Cell Biol / Involvement of an actomyosin contractile ring in Saccharomyces cerevisiaecytokinesis by Bi (1998)
10.1016/0014-5793(95)00580-3 / FEBS (Fed Eur Biochem Soc) Lett / Protein kinases—structure and function by Bossemeyer (1995)
{'key': '2023072901053561700_B13', 'first-page': '95', 'article-title': 'Start-specific transcription in yeast', 'volume': '208', 'author': 'Breeden', 'year': '1996', 'journal-title': 'Curr Top Microbiol Immunol'} / Curr Top Microbiol Immunol / Start-specific transcription in yeast by Breeden (1996)
{'key': '2023072901053561700_B14'}
10.1083/jcb.69.3.717 / J Cell Biol / A highly ordered ring of membrane-associated filaments in budding yeast by Byers (1976)
10.1083/jcb.143.3.709 / J Cell Biol / The septins are required for the mitosis-specific regulation of the Gin4 kinase by Carroll (1998)
10.1083/jcb.129.3.767 / J Cell Biol / Role of Bud3p in producing the axial budding pattern of yeast by Chant (1995)
10.1128/mr.59.3.345-386.1995 / Microbiol Rev / Molecular basis of cell integrity and morphogenesis in Saccharomyces cerevisiae. by Cid (1995)
10.1101/gad.10.22.2831 / Genes Dev / Two novel targets of the MAP kinase Kss1 are negative regulators of invasive growth in the yeast Saccharomyces cerevisiae. by Cook (1996)
10.1083/jcb.134.6.1345 / J Cell Biol / Septins may form a ubiquitous family of cytoskeletal elements by Cooper (1996)
10.1083/jcb.139.1.75 / J Cell Biol / A septin-based hierarchy of proteins required for localized deposition of chitin in the Saccharomyces cerevisiaecell wall by DeMarini (1997)
10.1099/13500872-142-10-2897 / Microbiology / SPR28, a sixth member of the septin gene family in Saccharomyces cerevisiaethat is expressed specifically in sporulating cells by De Virgilio (1996)
10.1128/MCB.15.10.5820 / Mol Cell Biol / Correlation of two-hybrid affinity data with in vitro measurements by Estojak (1995)
10.1126/science.276.5309.118 / Science / Bni1p, a yeast formin linking Cdc42p and the actin cytoskeleton during polarized morphogenesis by Evangelista (1997)
10.1083/jcb.132.3.399 / J Cell Biol / Identification of a developmentally regulated septin and involvement of the septins in spore formation in Saccharomyces cerevisiae. by Fares (1996)
10.1091/mbc.6.12.1843 / Mol Biol Cell / Localization and possible functions of Drosophilaseptins by Fares (1995)
10.1083/jcb.133.3.605 / J Cell Biol / A purified Drosophilaseptin complex forms filaments and exhibits GTPase activity by Field (1996)
10.1016/0168-9525(90)90012-U / Trends Genet / The two-hybrid system: an assay for protein-protein interactions by Fields (1994)
10.1083/jcb.122.2.373 / J Cell Biol / Components required for cytokinesis are important for bud site selection in yeast by Flescher (1993)
10.1002/dvg.1020120405 / Dev Genet / Cellular morphogenesis in the Saccharomyces cerevisiae cell cycle: localization of the CDC11gene product and the timing of events at the budding site by Ford (1991)
10.1083/jcb.143.3.737 / J Cell Biol / Polymerization of purified yeast septins: evidence that organized filament arrays may not be required for septin function by Frazier (1998)
10.1093/nar/20.6.1425 / Nucleic Acids Res / Improved method for high efficiency transformation of intact yeast cells by Gietz (1992)
10.1016/0378-1119(88)90185-0 / Gene / New yeast-Escherichia colishuttle vectors constructed with in vitro mutagenized yeast genes lacking six-base pair restriction sites by Gietz (1988)
10.1091/mbc.8.6.987 / Mol Biol Cell / Functional analysis of the interaction between Afr1p and the Cdc12p septin, two proteins involved in pheromone-induced morphogenesis by Giot (1997)
{'key': '2023072901053561700_B35'}
10.1016/0092-8674(93)90498-F / Cell / Cdi1, a human G1 and S phase protein phosphatase that associates with Cdk2 by Gyuris (1993)
{'key': '2023072901053561700_B37', 'first-page': '3678', 'article-title': 'Immunofluorescence localization of the Saccharomyces cerevisiae CDC12gene product to the vicinity of the 10-nm filaments in the mother-bud neck', 'volume': '7', 'author': 'Haarer', 'year': '1987', 'journal-title': 'Mol Cell Biol'} / Mol Cell Biol / Immunofluorescence localization of the Saccharomyces cerevisiae CDC12gene product to the vicinity of the 10-nm filaments in the mother-bud neck by Haarer (1987)
{'key': '2023072901053561700_B38', 'first-page': '576', 'article-title': 'The eukaryotic protein kinase superfamily: kinase (catalytic) domain structure and classification', 'volume': '9', 'author': 'Hanks', 'year': '1995', 'journal-title': 'FASEB (Fed Am Soc Exp Biol) J'} / FASEB (Fed Am Soc Exp Biol) J / The eukaryotic protein kinase superfamily: kinase (catalytic) domain structure and classification by Hanks (1995)
10.1016/0014-4827(71)90223-0 / Exp Cell Res / Genetic control of the cell division cycle in yeast. IV. Genes controlling bud emergence and cytokinesis by Hartwell (1971)
10.1083/jcb.112.4.535 / J Cell Biol / Cellular morphogenesis in the Saccharomyces cerevisiae cell cycle: localization of the CDC3gene product and the timing of events at the budding site by Kim (1991)
10.1016/0955-0674(94)90039-6 / Curr Opin Cell Biol / Cell cycle regulated transcription in yeast by Koch (1994)
10.1128/MCB.15.2.723 / Mol Cell Biol / AFR1 promotes polarized apical morphogenesis in Saccharomyces cerevisiae. by Konopka (1995)
10.1038/227680a0 / Nature / Cleavage of structural proteins during the assembly of the head of bacteriophage T4 by Laemmli (1970)
{'key': '2023072901053561700_B44', 'first-page': '3067', 'article-title': 'A yeast mitogen-activated protein kinase homolog (Mpk1p) mediates signalling by protein kinase C', 'volume': '13', 'author': 'Lee', 'year': '1993', 'journal-title': 'Mol Cell Biol'} / Mol Cell Biol / A yeast mitogen-activated protein kinase homolog (Mpk1p) mediates signalling by protein kinase C by Lee (1993)
10.1016/0955-0674(95)80028-X / Curr Opin Cell Biol / The proliferation of MAP kinase signaling pathways in yeast by Levin (1995)
10.1083/jcb.129.3.739 / J Cell Biol / A cell cycle checkpoint monitors cell morphogenesis in budding yeast by Lew (1995)
10.1128/jb.143.3.1384-1394.1980 / J Bacteriol / Reserve carbohydrate metabolism in Saccharomyces cerevisiae: response to nutrient limitation by Lillie (1980)
{'key': '2023072901053561700_B48'}
10.1016/S0955-0674(96)80054-8 / Curr Opin Cell Biol / The septins: roles in cytokinesis and other processes by Longtine (1996)
10.1002/(SICI)1097-0061(199807)14:10<953::AID-YEA293>3.0.CO;2-U / Yeast / Additional modules for versatile and economical PCR-based gene deletion and modification in Saccharomyces cerevisiae. by Longtine (1998)
10.1016/S0076-6879(96)66032-7 / Methods Enzymol / Prediction and analysis of coiled-coil structures by Lupas (1996)
10.1101/gad.10.11.1327 / Genes Dev / A search for proteins that interact genetically with histone H3 and H4 amino termini uncovers novel regulators of the Swe1 kinase in Saccharomyces cerevisiae. by Ma (1996)
{'key': '2023072901053561700_B53'}
10.1002/yea.320090705 / Yeast / Vectors for the inducible overexpression of glutathione S-transferase fusion proteins in yeast by Mitchell (1993)
10.1016/0092-8674(94)90152-X / Cell / The Drosophila peanutgene is required for cytokinesis and encodes a protein similar to yeast putative bud neck filament proteins by Neufeld (1994)
10.1046/j.1365-2443.1997.1590358.x / Genes Cells / Gin4 of S. cerevisiaeis a bud neck protein that interacts with the Cdc28 complex by Okuzaki (1997)
10.1016/S0091-679X(08)60320-9 / Methods Cell Biol / Methods for monitoring the growth of yeast cultures and for dealing with the clumping problem by Pringle (1975)
10.1016/0076-6879(91)94043-C / Methods Enzymol / Immunofluorescence methods for yeast by Pringle (1991)
10.1016/S0091-679X(08)61620-9 / Methods Cell Biol / Fluorescence microscopy methods for yeast by Pringle (1989)
10.1083/jcb.134.2.413 / J Cell Biol / The Bud4 protein of yeast, required for axial budding, is localized to the mother/bud neck in a cell cycle-dependent manner by Sanders (1996)
10.1083/jcb.136.1.95 / J Cell Biol / Targeting of chitin synthase 3 to polarized growth sites in yeast requires Chs5p and Myo2p by Santos (1997)
10.1073/pnas.76.2.645 / Proc Natl Acad Sci USA / Localized deposition of chitin on the yeast cell surface in response to mating pheromone by Schekman (1979)
10.1091/mbc.7.11.1657 / Mol Biol Cell / Cdc28 tyrosine phosphorylation and the morphogenesis checkpoint in budding yeast by Sia (1996)
10.1093/genetics/122.1.19 / Genetics / A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. by Sikorski (1989)
10.1083/jcb.96.2.486 / J Cell Biol / Filament ring formation in the dimorphic yeast Candida albicans. by Soll (1983)
10.1083/jcb.128.3.263 / J Cell Biol / PICK1: a perinuclear binding protein and substrate for protein kinase C isolated by the yeast two-hybrid system by Staudinger (1995)
10.1046/j.1365-2443.1996.d01-213.x / Genes Cells / Nik1: a Nim1-like protein kinase of S. cerevisiaeinteracts with the Cdc28 complex and regulates cell cycle progression by Tanaka (1996)
{'key': '2023072901053561700_B68', 'first-page': '1255', 'article-title': 'How do protein kinases discriminate between serine/threonine and tyrosine? Structural insights from the insulin receptor protein-tyrosine kinase', 'volume': '9', 'author': 'Taylor', 'year': '1995', 'journal-title': 'FASEB (Fed Am Soc Exp Biol) J'} / FASEB (Fed Am Soc Exp Biol) J / How do protein kinases discriminate between serine/threonine and tyrosine? Structural insights from the insulin receptor protein-tyrosine kinase by Taylor (1995)
10.1111/j.1365-2958.1991.tb01993.x / Mol Microbiol / A protein kinase gene complements the lytic phenotype of Saccharomyces cerevisiae lyt2mutants by Torres (1991)
10.1002/j.1460-2075.1993.tb05845.x / EMBO (Eur Mol Biol Organ) J / Comparison of the Saccharomyces cerevisiaeG1 cyclins: Cln3 may be an upstream activator of Cln1, Cln2 and other cyclins by Tyers (1993)
10.1126/science.1496382 / Science / A protein kinase substrate identified by the two-hybrid system by Yang (1992)
10.1016/0092-8674(93)90662-A / Cell / Mxi1, a protein that specifically interacts with Max to bind Myc-Max recognition sites by Zervos (1993)

Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 12:47 p.m.)
Deposited	2 years, 1 month ago (July 28, 2023, 9:07 p.m.)
Indexed	2 months ago (July 3, 2025, 10:26 p.m.)
Issued	26 years, 10 months ago (Nov. 2, 1998)
Published	26 years, 10 months ago (Nov. 2, 1998)
Published Online	26 years, 10 months ago (Nov. 2, 1998)
Published Print	26 years, 10 months ago (Nov. 2, 1998)

Funders 0

None

BibTeX

@article{Longtine_1998, title={Role of the Yeast Gin4p Protein Kinase in Septin Assembly and the Relationship between Septin Assembly and Septin Function}, volume={143}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.143.3.719}, DOI={10.1083/jcb.143.3.719}, number={3}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Longtine, Mark S. and Fares, Hanna and Pringle, John R.}, year={1998}, month=nov, pages={719–736} }

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      "first-page": "934",
      "DOI": "10.1083/jcb.98.3.934",
      "article-title": "Relationship of actin and tubulin distribution to bud growth in wild-type and morphogenetic-mutant Saccharomyces cerevisiae.",
      "volume": "98",
      "author": "Adams",
      "year": "1984",
      "journal-title": "J Cell Biol"
    },
    {
      "key": "2023072901053561700_B3",
      "doi-asserted-by": "crossref",
      "first-page": "267",
      "DOI": "10.1007/s004380050415",
      "article-title": "Screening and identification of yeast sequences that cause growth inhibition when overexpressed",
      "volume": "254",
      "author": "Akada",
      "year": "1997",
      "journal-title": "Mol Gen Genet"
    },
    {
      "key": "2023072901053561700_B4",
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      "first-page": "119",
      "DOI": "10.1083/jcb.138.1.119",
      "article-title": "Control of mitotic events by Nap1 and the Gin4 kinase",
      "volume": "138",
      "author": "Altman",
      "year": "1997",
      "journal-title": "J Cell Biol"
    },
    {
      "key": "2023072901053561700_B5"
    },
    {
      "key": "2023072901053561700_B6",
      "doi-asserted-by": "crossref",
      "first-page": "399",
      "DOI": "10.1083/jcb.137.2.399",
      "article-title": "High rates of actin filament turnover in budding yeast and roles for actin in establishment and maintenance of cell polarity revealed using the actin inhibitor latrunculin-A",
      "volume": "137",
      "author": "Ayscough",
      "year": "1997",
      "journal-title": "J Cell Biol"
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      "first-page": "249",
      "DOI": "10.1128/MMBR.62.2.249-274.1998",
      "article-title": "Signalling in the yeasts: an informational cascade with links to the filamentous fungi",
      "volume": "62",
      "author": "Banuett",
      "year": "1998",
      "journal-title": "Microbiol Mol Biol Rev"
    },
    {
      "key": "2023072901053561700_B8",
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      "first-page": "3329",
      "DOI": "10.1093/nar/21.14.3329",
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      "author": "Baudin",
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      "author": "Benton",
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      "volume": "143",
      "author": "Carroll",
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      "volume": "59",
      "author": "Cid",
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      "author": "Sikorski",
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