DOI: 10.1083/jcb.143.1.135. Clb5-associated Kinase Activity is Required Early in the Spindle Pathway for Correct Preanaphase Nuclear Positioning in <i>Saccharomyces cerevisiae </i>

Clb5-associated Kinase Activity is Required Early in the Spindle Pathway for Correct Preanaphase Nuclear Positioning in Saccharomyces cerevisiae

10.1083/jcb.143.1.135

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

In Saccharomyces cerevisiae, a single cyclin-dependent kinase, Cdc28, regulates both G1/S and G2/M phase transitions by associating with stage-specific cyclins. During progression through S phase and G2/M, Cdc28 is activated by the B-type cyclins Clb1–6. Because of functional redundancy, specific roles for individual Clbs have been difficult to assign. To help genetically define such roles, strains carrying a cdc28ts allele, combined with single CLB deletions were studied. We assumed that by limiting the activity of the kinase, these strains would be rendered more sensitive to loss of individual Clbs. By this approach, a novel phenotype associated with CLB5 mutation was observed. Homozygous cdc28-4ts clb5 diploids were inviable at room temperature. Cells were defective in spindle positioning, leading to migration of undivided nuclei into the bud. Occasionally, misplaced spindles were observed in cdc28-4 clb5 haploids; additional deletion of CLB6 caused full penetrance. Thus, CLB5 effects proper preanaphase spindle positioning, yet the requirement differs in haploids and diploids. The execution point for the defect corresponded to the time of Clb5-dependent kinase activation. Nevertheless, lethality of cdc28-4 clb5 diploids was not rescued by CLB2 or CLB4 overexpression, indicating a specificity of Clb5 function beyond temporality of expression.

Bibliography

Segal, M., Clarke, D. J., & Reed, S. I. (1998). Clb5-associated Kinase Activity is Required Early in the Spindle Pathway for Correct Preanaphase Nuclear Positioning in Saccharomyces cerevisiae . The Journal of Cell Biology, 143(1), 135â145.

Authors 3

Marisa Segal (first)
Duncan J. Clarke (additional)
Steven I. Reed (additional)

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 12:47 p.m.)
Deposited	2 years, 1 month ago (July 28, 2023, 8:13 p.m.)
Indexed	1 month, 3 weeks ago (July 9, 2025, 6:47 p.m.)
Issued	26 years, 10 months ago (Oct. 5, 1998)
Published	26 years, 10 months ago (Oct. 5, 1998)
Published Online	26 years, 10 months ago (Oct. 5, 1998)
Published Print	26 years, 10 months ago (Oct. 5, 1998)

Funders 0

None

BibTeX

@article{Segal_1998, title={Clb5-associated Kinase Activity is Required Early in the Spindle Pathway for Correct Preanaphase Nuclear Positioning in Saccharomyces cerevisiae }, volume={143}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.143.1.135}, DOI={10.1083/jcb.143.1.135}, number={1}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Segal, Marisa and Clarke, Duncan J. and Reed, Steven I.}, year={1998}, month=oct, pages={135–145} }

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