DOI: 10.1083/jcb.142.6.1605. The Membrane-proximal Region of the E-Cadherin Cytoplasmic Domain Prevents Dimerization and Negatively Regulates Adhesion Activity

The Membrane-proximal Region of the E-Cadherin Cytoplasmic Domain Prevents Dimerization and Negatively Regulates Adhesion Activity

10.1083/jcb.142.6.1605

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

Cadherins are transmembrane glycoproteins involved in Ca2+-dependent cell–cell adhesion. Deletion of the COOH-terminal residues of the E-cadherin cytoplasmic domain has been shown to abolish its cell adhesive activity, which has been ascribed to the failure of the deletion mutants to associate with catenins. Based on our present results, this concept needs revision. As was reported previously, leukemia cells (K562) expressing E-cadherin with COOH-terminal deletion of 37 or 71 amino acid residues showed almost no aggregation. Cells expressing E-cadherin with further deletion of 144 or 151 amino acid residues, which eliminates the membrane-proximal region of the cytoplasmic domain, showed E-cadherin–dependent aggregation. Thus, deletion of the membrane-proximal region results in activation of the nonfunctional E-cadherin polypeptides. However, these cells did not show compaction. Chemical cross-linking revealed that the activated E-cadherin polypeptides can be cross-linked to a dimer on the surface of cells, whereas the inactive polypeptides, as well as the wild-type E-cadherin polypeptide containing the membrane-proximal region, can not. Therefore, the membrane-proximal region participates in regulation of the adhesive activity by preventing lateral dimerization of the extracellular domain.

Bibliography

Ozawa, M., & Kemler, R. (1998). The Membrane-proximal Region of the E-Cadherin Cytoplasmic Domain Prevents Dimerization and Negatively Regulates Adhesion Activity. The Journal of Cell Biology, 142(6), 1605â1613.

Authors 2

Masayuki Ozawa (first)
Rolf Kemler (additional)

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 12:47 p.m.)
Deposited	2 years, 1 month ago (July 22, 2023, 3:57 a.m.)
Indexed	3 months, 1 week ago (May 19, 2025, 8:30 a.m.)
Issued	26 years, 11 months ago (Sept. 21, 1998)
Published	26 years, 11 months ago (Sept. 21, 1998)
Published Online	26 years, 11 months ago (Sept. 21, 1998)
Published Print	26 years, 11 months ago (Sept. 21, 1998)

Funders 0

None

BibTeX

@article{Ozawa_1998, title={The Membrane-proximal Region of the E-Cadherin Cytoplasmic Domain Prevents Dimerization and Negatively Regulates Adhesion Activity}, volume={142}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.142.6.1605}, DOI={10.1083/jcb.142.6.1605}, number={6}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Ozawa, Masayuki and Kemler, Rolf}, year={1998}, month=sep, pages={1605–1613} }

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