DOI: 10.1083/jcb.142.6.1595. The Symmetrical Structure of Structural Maintenance of Chromosomes (SMC) and MukB Proteins: Long, Antiparallel Coiled Coils, Folded at a Flexible Hinge

The Symmetrical Structure of Structural Maintenance of Chromosomes (SMC) and MukB Proteins: Long, Antiparallel Coiled Coils, Folded at a Flexible Hinge

10.1083/jcb.142.6.1595

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

Structural maintenance of chromosomes (SMC) proteins function in chromosome condensation and several other aspects of DNA processing. They are large proteins characterized by an NH2-terminal nucleotide triphosphate (NTP)-binding domain, two long segments of coiled coil separated by a hinge, and a COOH-terminal domain. Here, we have visualized by EM the SMC protein from Bacillus subtilis (BsSMC) and MukB from Escherichia coli, which we argue is a divergent SMC protein. Both BsSMC and MukB show two thin rods with globular domains at the ends emerging from the hinge. The hinge appears to be quite flexible: the arms can open up to 180°, separating the terminal domains by 100 nm, or close to near 0°, bringing the terminal globular domains together. A surprising observation is that the ∼300–amino acid–long coiled coils are in an antiparallel arrangement. Known coiled coils are almost all parallel, and the longest antiparallel coiled coils known previously are 35–45 amino acids long. This antiparallel arrangement produces a symmetrical molecule with both an NH2- and a COOH-terminal domain at each end. The SMC molecule therefore has two complete and identical functional domains at the ends of the long arms. The bifunctional symmetry and a possible scissoring action at the hinge should provide unique biomechanical properties to the SMC proteins.

Bibliography

Melby, T. E., Ciampaglio, C. N., Briscoe, G., & Erickson, H. P. (1998). The Symmetrical Structure of Structural Maintenance of Chromosomes (SMC) and MukB Proteins: Long, Antiparallel Coiled Coils, Folded at a Flexible Hinge. The Journal of Cell Biology, 142(6), 1595â1604.

Authors 4

Thomas E. Melby (first)
Charles N. Ciampaglio (additional)
Gina Briscoe (additional)
Harold P. Erickson (additional)

References 34 Referenced 316

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{'key': '2023072207590590500_B4'}
10.1016/0022-2836(79)90034-2 / J Mol Biol / Trinodular structure of fibrinogen. Confirmation by both shadowing and negative stain electron microscopy by Fowler (1979)
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10.1016/0955-0674(95)80087-5 / Curr Opin Cell Biol / The SMC family: from chromosome condensation to dosage compensation by Hirano (1995)
10.1016/S0092-8674(00)80233-0 / Cell / Condensins, chromosome condensation protein complexes containing XCAP-C, XCAP-E and a Xenopus homolog of the Drosophilabarren protein by Hirano (1997)
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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 12:47 p.m.)
Deposited	2 years, 1 month ago (July 22, 2023, 4 a.m.)
Indexed	3 weeks ago (Aug. 7, 2025, 4:52 p.m.)
Issued	26 years, 11 months ago (Sept. 21, 1998)
Published	26 years, 11 months ago (Sept. 21, 1998)
Published Online	26 years, 11 months ago (Sept. 21, 1998)
Published Print	26 years, 11 months ago (Sept. 21, 1998)

Funders 0

None

BibTeX

@article{Melby_1998, title={The Symmetrical Structure of Structural Maintenance of Chromosomes (SMC) and MukB Proteins: Long, Antiparallel Coiled Coils, Folded at a Flexible Hinge}, volume={142}, ISSN={1540-8140}, url={http://dx.doi.org/10.1083/jcb.142.6.1595}, DOI={10.1083/jcb.142.6.1595}, number={6}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Melby, Thomas E. and Ciampaglio, Charles N. and Briscoe, Gina and Erickson, Harold P.}, year={1998}, month=sep, pages={1595–1604} }

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